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corneas
Rhesus putative epidermal stem cells are being investigated for their potential use in regenerative corneal epithelium-like cells, which may provide a practical source of autologous seed cells for the construction of bioengineered corneas.
      
To develop a rabbit corneal endothelial (RCE) cell line, in vitro culture of RCE cells was initiated from Oryctolagus curiculus corneas and a novel RCE cell line was established in this study.
      
To initiate the primary culture of RCE cells, corneas from rabbit eyes were sliced and attached into glutin-coated wells with endothelial cell surface down.
      
Corneal antigens were prepared from donor corneas not suitable for surgery.
      
Samples of a PHEMA sponge were implanted in rabbit corneas and explanted at predetermined time points (2, 4, and 12 weeks).
      
Under electron microscopy, the lymphatic vessels in the rat injured corneas were examined.
      
The results showed that the lymphatic vessels were found in the injured rat corneas 14 days after the injury.
      
The VEGF-C mRNA was minimally detected in the normal rat corneas, but it was highly expressed 5 days after the injury.
      
Among 12 rats in each group, the corneas of 2 rats in each group were used for pathological study at day 14 after the transplantation, and the remaining 10 rats were used for studying corneal rejection by a slit lamp.
      
Lymphangiogenesis occurring in transplanted corneas
      
In addition, 19 allograft failed human corneas were examined by 5'-nase-alkaline phosphatase (5'-NA-ALP) double-enzyme-histochemistry staining to detect corneal lymphangiogenesis and hemangiogenesis.
      
By 5'-NA-ALP enzyme-histochemistry, corneal hemangiogenesis was found in all allograft failed human corneas and 5 of 19 (26.
      
Distribution of catecholaminergic nerve fibers in normal and alkali-injured rabbit corneas
      
The alkali-burned corneas of 12 rabbits were studied with fluorescence microscopy 1 week, 3 weeks, and 6 months after injury to identify the arrangement of catecholaminergic nerve fibers (CNF) and related levels of norepinephrine.
      
In the wounded corneas, CNF were reduced at both 1 and 3 weeks and were restored by 6 months, as shown by histofluorescent staining.
      
Ofloxacin-treated corneas yielded an average amount of colony-forming units (CFUs) of P aeruginosa that was statistically significantly higher than that of ciprofloxacin-treated corneas (4.7×104±2.2×103 vs 2.5×103±1.0×102).
      
Histopathologic findings from the excised corneas of both patients were consistent with macular corneal dystrophy.
      
This study evaluated immediate and short-term histologic modifications caused by myopic intrastromal photorefractive keratectomy (IPRK) with the neodymium:yttrium lithium fluoride (ND:YLF) picosecond laser in human corneas.
      
After treatment with this technique, 2 corneas of patients' blind eyes were enucleated and examined with optical and electronic transmission and scanning microscopy.
      
The corneas did not present clearly visible structural modifications in the adjacent treatment areas, and they regained their morphologic characteristics 72 hours after treatment.
      
 

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