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the correct orientation
Recombinant transposing vector pFHIV24 was constructed by cloning the HIV-1 p24 gene into the Multiple cloning site (MCS) of the transposing vector pFastBac1 in the correct orientation with respect to the polyhedrin promoter.
      
High levels of expression induced by galactose were observed with pBCLG2, which bears the LAC4 gene in the correct orientation, while a low constitutive level of expression was observed in pBCLG4 transformants both in glucose and in galactose media.
      
High levels of expression induced by galactose were observed with pBCLG2, which bears the LAC4 gene in the correct orientation, while a low constitutive level of expression was observed in pBCLG4 transformants both in glucose and in galactose media.
      
The correct orientation of a large segment (at least 200 cM) on the left arm of chromosome I of S.
      
Both the sets of animals (T and G) show systematic differences from the correct orientation between the period when the moon culminates South (series of experiments with negative lunar declination: Figs.
      
1974), integrates site-specifically and in the correct orientation at the preferential attachment site generated by Rac excision.
      
Tumors containing the NPT II gene in the correct orientation relative to the promoter were able to grow on normally inhibitory concentrations of the antibiotic G418.
      
DNA sequence analysis of the recombinant plasmid, pMU1, revealed that a 1514 by cDNA was inserted in the correct orientation and in-frame with the start of the lacZ coding sequence in the vector, pUC18.
      
Our observations support our expectations that a functional cis element must provide a template to which two TNPA molecules can bind in the correct orientation.
      
The mechanism by which the CD ampullae find the correct orientation is currently unknown.
      
The accuracy of joint direction is one of the more important factors in determining the correct orientation of underground excavations.
      
These results suggest that MFs are involved in maintaining the SMC nucleus in its correct position and the SMC spindle in the correct orientation relative to the division site previously delineated by the preprophase band.
      
The extended element (-1364, -947) functions in both orientations from 5' locations whereas the SPE2 subcomponent (-1364, -1154) containing the conserved sequence is only active in the correct orientation.
      
Expression of the lacZ gene depends on the presence of an active promoter outside of the transposon in the correct orientation.
      
lipoferum were less for pBTF8, which carries thecryIA(a) gene in the correct orientation for a constitutive promoter, than for pBTF9, which carries the gene in the opposite orientation.
      
On the other hand, the docking of diazepam to α1/γ2 interface revealed five multi-member conformational clusters in the binding site and model 1 seemed to represent the correct orientation of diazepam in the binding site.
      
Apparently, none of the four highest orientation peaks in the full model-based approach corresponds to the correct orientation.
      
Again, the correct orientation of the inserted intron DNA was confirmed by digestion with SacI.
      
A fine grid search for C using Como28 then located the correct orientation and position of this monomer.
      
A clone was identified with the GUS gene in the correct orientation, and its sequence was determined to be correct.
      
 

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