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three changes
Comparison of the allele sequences revealed three changes: two (codons 308 and 437) are silent exonic substitutions, one is a G to A transition corresponding to an amino acid difference in position 282: Val (GTG) in G2mn-, Met (ATG) in G2mn+.
      
The cartilage specimens were extracted sequentially with three changes of neutral buffer, three changes of 6 M guanidine hydrochloride and then partiallydegraded with bacterial collagenase.
      
A succession of two or three changes in the changes in the total pattern during training, or even a single pattern is sufficient for a concept to develop.
      
Blocks of tissues were fixed in Carnoy's fluid overnight, transferred to absolute alcohol in the morning and washed in three changes of alcohol for a total of 6-8 hours.
      
Activation of crossing inhibitory neurons proved a sufficient mechanism to explain all three changes in the locomotor rhythm during a fictive turn.
      
The mutation (G(-169) → A) resulted in a 50% decrease in expression from the promoter (Pu), whereas the other three changes had no significant effect.
      
This improved method requires only a small number of seeds for explant preparation, and three changes of medium; the overall transformation efficiency achieved, based on the recovery of transformed plants after 4-5 months of culture, reached 8-9%.
      
The second trajectory involves three changes, six intermediate sequences, and six potentially equally parsimonious paths.
      
Compensation in both groups was achieved through three changes in the Pmus waveform.
      
Hybrid cells proliferated and clones were isolated after 2 to 3 weeks growth in three changes of HAT-AME medium and subsequent growth in HAT medium alone.
      
These include changes to three highly, but not completely, conserved residues in the pocket of the active site and also three changes (including deletions) to the active site loop.
      
There are also three changes in glycosylation status.
      
In pancreatic acinar cells, the reaction product for the enzyme activity was not found even after 90 min of incubation with three changes of the medium.
      
Seedlings receiving single applications were left in the treatment solutions for two days and then harvested, while seedlings receiving multiple applications had their solutions changed every other day for a total of three changes.
      
We examine the effect of these three changes on the germination ofN.
      
We have found that by using a common chemical deglycosylation technique, one can effect at least three changes in a peptide's structure: methylation, isomerization, and ring formation.
      
The integrated intensity of the electronic background between 200 and 700 cm-1 observed in the Raman spectra of the YBa2Cu3O7-x 1-2-3 system exciting at 1.16 eV shows three changes with temperature.
      
In particular, adding a single directed edge to nearest neighbor random walk on a finite discrete torus in dimensions one, two, or three changes the stationary distribution linearly, logarithmically, or only locally.
      
Zona-encased oocytes/embryos were exposed to antisera for 30 min at room temperature, followed by washing in three changes of PBS.
      
After washing in three changes ofa 0.9% NaCl solution, cell layers underwent two sequential precipitations with cold 5% TCA, for 10 min each.
      
 

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