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cdna
The cDNA fragment was inserted into the expression vector pET-28a, and the resulting plasmid was expressed in Escherichia coli BL21 (DE3) by isopropylthiogalactoside induction.
      
The site-mutated RI cDNA was constructed into plasmid pPIC9K and then transformed Pichia pastoris GS115 by electroporation.
      
Modification of the full-length cDNA clone of Newcastle disease virus isolated from an outbreak in the goose
      
Finally, the corresponding fragment in the mutant full-length cDNA was substituted with the new one.
      
The sequencing results showed that the three additional G nts were successfully inserted and the mutant nts in the full-length cDNA were corrected.
      
The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers on the basis of the ZNF322 sequence analyzed with computer.
      
The cDNA of PhPI9 has a fragment of 834 bp and has 60% identity with the PISTILATA from Arabidopsis.
      
The murine IL-23 cDNA was sub-cloned into a dual-expression vector.
      
To construct a polymerase chain reaction (PCR) site-directed mutagenesis of the long QT syndrome KCNQ1 gene in vitro, two sets of primers were designed according to the sequence of KCNQ1 cDNA and a mismatch was introduced into primers.
      
Mutation from A to G in site 983 of KCNQ1 cDNA was found.
      
The pGEMT-easy vector containing the full-length cDNA encoding human podocin was cloned and digested with BamHI and XhoI.
      
Structural analysis of the PCR products synthesized on cDNA-5°C and cDNA-18°C has revealed no differences.
      
cDNA libraries obtained from the native and regenerating retina were used as templates.
      
For the first time, primers constructed on the basis of OCT4 and NANOG mRNAs were used for PCR analysis of cDNA derived from the eyes of a 9.5-week human fetus.
      
Using cDNA derived from the whole eyes of 8-, 9-, 10.5-, and 11-week fetuses, the expression of two PITX2 isoforms specific for eye tissues (A and B) was revealed.
      
cDNA of human peroxiredoxin VI, one of the recently discovered novel antioxidant proteins, was expressed in Escherichia coli cells.
      
The amount of cDNA of these genes was evaluated using multiplex RT-PCR.
      
The cDNA encoding cytochrome P-45017α from bovine adrenal cortex was expressed in Saccharomyces cerevisiae under the control of the galactose-inducible GAL10 promoter.
      
The corresponding cDNA fragment has been cloned and expressed in E.
      
However, specific activity of DL isolated from such cells was significantly lower than that of enzyme purified from extracts of Spodoptera frugiperda cells, which were infected with the baculovirus vector carrying DL cDNA.
      
 

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