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indirect labelling
The directly 15N-labelled casuarina residue and indirect labelling (unlabelled casuarina + 15N soil) were compared to estimate the proportion and amount of N derived from the residue and fertilizer.
      
Using the indirect labelling PAP-technique, specific immunostaining was found in the endothelial layer of all arteries and arterioles of kidney cortex and in some descending vasa recta.
      
The indirect labelling of antibodies, with the avidin-biotin amplifying system, should make this technique suitable for the quantitation of other plant growth substances against which specific antibodies are available.
      
Direct and indirect labelling procedures were employed using colloidal gold and ferritin-labelled lectins, or biotinylated lectins followed by gold-labelled streptavidin.
      
by means of a MiniMACS separation unit after indirect labelling with the antibody mAb414 (raised against rat liver nucleoporin p62) and a secondary anti-mouse antibody labelled with superparamagnetic MACS Microbeads.
      
In this assay, we compare both direct and indirect labelling methods by utilizing either iodinated annexin V or biotinylated annexin V/[35S] streptavidin to radiolabel apoptotic cells.
      
However, this is generally not a problem, due to the indirect labelling method employed.
      
Indirect labelling resulted in greater absolute uptake and prolonged intracellular retention of the radiolabel, when compared with direct labelling.
      
In preliminary experiments, thin sections of zoospores and cysts were labelled using an indirect labelling procedure.
      
In the case of direct labelling, the retained intracellular radioactivity was 29-31% after 24 h, whereas for indirect labelling it was 49-61%.
      
Phenotypic expression of surface molecules was determined by direct and indirect labelling using standard protocols.
      
The indirect labelling resulted in stronger binding of the mAb as well as prolonged intracellular retention of the radiolabel.
      
The results of this cellular study indicate that indirect labelling is preferable to the direct Chloramine-T method.
      
The results show a pronounced retention of internalized radioactivity in the case of indirect labelling as compared with direct labelling.
      
 

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