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Prodigiosin also could induce apoptosis of pancreatic cancer cells at low concentration and results in the fragmentation pattern of DNA.
      
All these results demonstrate that prodigiosin can obviously inhibit the proliferation of pancreatic cancer cells H8898 by arresting the cell cycle and inducing apoptosis.
      
Detection of the apoptosis of Jurkat cell using an electrorotation chip
      
The apoptosis of cells is one of the fields that attract increasing attention in biology today.
      
Usually, the cells are treated with chemicals when detecting apoptosis.
      
It is highly desired to detect apoptosis in a real-time basis.
      
Apoptosis of Jurkat cells was studied using a real-time electrorotation chip.
      
This chip allows the detection of the cell membrane capacitance changes during the course of apoptosis and therefore facilitates the analysis of apoptosis in a real-time basis without involving any chemical treatment.
      
Flow cytometry (FCM) demonstrated that MDA triggered cells to undergo apoptosis, in parallel with the findings in MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay which showed that it can also impair cellular viability.
      
Annexin V/PI bivariate flow cytometric analysis further revealed that the five extracts significantly induced early apoptosis in HL-60 cells.
      
lucidum but also other Ganoderma species can inhibit cancer cells, and their mechanisms are related to induction of apoptosis.
      
In these primary experiments, it showed that 6HRE-GFAP-Baxα system could promote glioma cell apoptosis.
      
Absence of FHIT expression is associated with apoptosis inhibition in colorectal cancer
      
In our present study we tested the hypothesis that the decreased FHIT expression resulted in apoptosis inhibition associated with abnormal expression of apoptosis related proteins.
      
In the first test, we assessed apoptosis status using a standard TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling) assay by comparing FHIT-positive CRC vs.
      
In the second experiment, the protein expression of the FHIT and other apoptosis related proteins (Bax, Bcl-2 and Survivin) were measured in human CRC by TMA.
      
Both TUNEL and TMA experiments demonstrated significantly inhibited apoptosis by down-regulation of Bax and the up-regulation of Survivin and Bcl-2.
      
Apoptosis was detected by flow cytometry and cell proliferation was evaluated by MTT assay.
      
Seventy-two hours after transfection, apoptosis rates of the two RNAi groups were 21.51% and 26.28%, both of which were higher than control Lovo's (9.03%).
      
Based on the results, we can draw a conclusion that the two survivin-targeted siRNAs successfully suppressed the expression of survivin mRNA, inhibited cell growth and induce cell apoptosis.
      
 

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