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centromere
The results were validated by using fluorescent in situ hybridization (FISH) analysis with centromere-specific DNA probes.
      
Computer-aided analysis of the amplified in TAIL-RCR DNA region adjacent to the left border of the insertion revealed a putative site of T-DNA insertion, the 2.5-kb At2g09920 gene located in the long arm of chromosome 2, near the centromere.
      
If the mating-type locus is linked to the centromere, the genome regions adjoining the centromeres of all chromosomes remain heterozygous.
      
Genetic regulation of the centromere division in rye and wheat univalent chromosomes in dimonosomics during the meiotic anaphase
      
The data obtained demonstrate that the rye and wheat chromosomes studied are involved in genetic regulation of centromere division in meiotic anaphase I (AI).
      
The translocation breakpoint is located between Xpsr680 and Xpsr965 about 90-99 cM from the centromere.
      
In the present study, the high frequency of centromeric rearrangements indicates a potential role for mitotic irregularities associated with the centromere in prostate cancer tumorigenesis.
      
Using this technique, RFLP marker umc58 closely linked with the hm1 gene dictatingHelminthosporium carbonum susceptibility1 was localized onto 1L3 (chromosome 1, long arm, the third band from the centromere to the end of the arm), 5L5 and 9L5.
      
A fluorescencein situ hybridization (FISH) procedure was adopted to physically map a RFLP marker, umc119 near the centromere of the long arm of linkage group1 in maize.
      
The percentage distance from centromere to the hybridization site was 22.86 on 1 L and 58.23 on 5 L the detection rate was about 12% for mitotic cells.
      
However, in these species, the NOR is located proximal to the centromere on the short arm, while in Pl.
      
In contrast, C-banding revealed a heterochromatic block near the centromere on the short arm of pair 3, a characteristic common to all members of this group of Physalaemus.
      
These serum samples were also evaluated for anti-centomere antibodies by anti-centromere ELISA kit.
      
The anti-centromere antibodies were significantly higher in patients with CREST only.
      
We found that the chromosome volume distribution agreed with DNA length distribution (obtained from a public database), and that the short arm to long arm volume ratio showed good agreement with the genomic position of the centromere.
      
Immunological characterization of heterochromatin protein p25β autoantibodies and relationship with centromere autoantibodies an
      
In western blotting, some ACA-positive sera also recognize a doublet of 23?kDa (p23) and 25?kDa (p25) in addition to centromere protein antigens A (17?kDa), B (80?kDa), and C (140?kDa).
      
The major targets recognized by anti-centromere autoantibodies are the three centromere-associated proteins (CENPs) A, B, and C, with apparent molecular masses of 19, 80, and 140?kDa, respectively.
      
Probing of the membranes with various anti-centromere sera showed that all epitopes are clustered in the N-terminal 45 amino acids.
      
Recently we have shown that the anti-centromere-associated protein A (CENP-A) immune response is directed against an autoantigenic motif, G/A-P-R/S-R-R, that occurs three times in the N-terminal amino acids of CENP-A.
      
 

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