助手标题
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
共[1]页 共[18]条 当前为第1条到18条
 

相关语句
pcineo
We have constructed a bicistronic vector, pCINeo/IRES-GFP, which utilises a red-shifted variant of Green Fluorescent Protein as an in vivo cell marker.
      
It is concluded that the bicistronic pCINeo/IRES-GFP vector provides an efficient and non-invasive way of identifying cells which express ion channels after transfection.
      
The expression of the different domains of a complex serine protease, recombinant Factor C of the horseshoe crab, Carcinoscorpius rotundicauda, was achieved using pCIneo and pEGFP-N1 vectors.
      
In an in vitro coupled transcription-translation system, aberrant products were generated from pCDNAI/CrFC21, as opposed to pCIneo/CrFC21 which gave rise to the expected polypeptide.
      
COS-1 cells transfected with pCIneo/CrFC21 expressed Factor C-related proteins which differ from those produced in the yeast, Pichia pastoris.
      
Effects of pCIneo and pCEP4 expression vectors on transient and stable protein production in human and simian cell lines
      
Conventional vectors such as pCIneo and pcDNA3 have been used frequently.
      
In preliminary experiments, we compared transient secreted placental alkaline phosphatase production in 8 cell lines from 3 different species using the pCIneo vs.
      
Stable pools of cells (uncloned) expressing human hepatocyte growth factor were isolated using pCIneo and pCEP4 and protein production levels were compared to those seen in transient transfections.
      
In this study primary cultures of human foetal cerebral cortex cells were transfected, using cationic lipids, with a eukaryotic expression vector (pCIneo-THI) containing the cDNA for human tyrosine hydroxylase isoform I (TH).
      
Cortical cells from human (10-14 week) foetuses were cultured for 11 days in vitro and transfected twice with pCIneo-TH1 during this time.
      
Expression plasmids derived from pCINeo have been shown to produce increased amounts of RNA that enhance expression upon transfection.
      
Expression of the F protein was not detected when pCIneoL had been replaced by pCIneo in the cotransfection experiments.
      
However, H13-pCIneo generated only four seropositive birds out of eight vaccinated birds, and H14pCIneo did not produce any seropositive birds.
      
Transfection with p1-FRI or pCIneo has no effect on the activity of any of the promoters.
      
These chimeric constructs were cloned into pCINeo vectors and were expressed stably in SKT6 cells.
      
The complete coding sequence of the HA genes from 17 different influenza viruses were cloned into pCIneo EEVs.
      
The identified mCCR8 sequence was subcloned into pCIneo to generate the corresponding expression vector.
      
 

首页上一页1下一页尾页 

 
CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社