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comet assay
The single cell gel electrophoresis or comet assay was used to quantify DNA strand breaks as a measure of DNA damage induced by Cd and imidacloprid contamination in soil.
      
Roots ofVicia faba were exposed to the contaminated soil for 2 h at 25°C and were used in the comet assay.
      
By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded.
      
Using the alkaline comet assay, we showed that antioxidants - vitamins C and E, quercetin, and melatonin - reduced the genotoxic effect of MNNG in H.
      
Carotenoid concentrations of lymphocytes were determined by HPLC and DNA damage was evaluated by the comet assay following an ex vivo treatment with H2O2.
      
Optimization and Standardization of the "Comet Assay" for Analyzing the Repair of DNA Damage in Cells
      
The "comet assay" has become an interesting and a very useful tool for the analysis of the induction and amount of DNA damage in single cells thus offering the opportunity to measure the effectiveness of DNA repair.
      
This paper attempts a correlation between the induction and repair of DNA damage measured in the comet assay and the clinical observed reaction in order to evaluate the suitability of the comet assay for prediction of radiation sensitivity.
      
Analysis of the Action of the Restriction Endonuclease AluI Using Three Different Comet Assay Protocols
      
Radioprotective Effects of Amifostine In Vitro and In Vivo Measured with the Comet Assay
      
In this study, the preventive, suppressive, and protective effects of in vitro supplementation with electrolyzed-reduced water on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay.
      
The Comet assay technique was also performed for the highest concentrations of the two mentioned metal ions as an index of DNA breaks.
      
The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells.
      
The apoptotic nuclei of LoVo cells induced by MAP30 were obviously observed, and the genomic degradation was detected by single-cell gel electrophoresis (comet assay).
      
Statistical Analysis of the Comet Assay Using a Mixture of Gamma Distributions
      
Tail moments in the single cell gel electrophoresis (comet) assay usually do not follow a normal distribution, making the statistical analysis complicated.
      
The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay
      
In this study, two common pollutants (benzo[a]pyrene and methamidophos) in marine environment were tested by comet assay for their inducement ofin vivo genotoxic effect to the blood cells of black porgy (Acanthopagrus schlegeli).
      
In addition, DNMT1 siRNAs showed an inhibitory effect of cell proliferation in the cancer cells and the induction of cell death without evidence of DNA damage, whereas treatment with 5-aza-dC caused DNA damage as demonstrated by the comet assay.
      
Using the comet assay, we found that Dynabead-coated beta cells showed equal susceptibility to cytokine-induced DNA damage as non-coated cells.
      
 

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