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media changes
from mass media) changes the ratio between minority and majority, until above a critical external support the supported subpopulation exists always as a majority.
      
After incubation for 16 to 18 h at 37 °C and the addition of only one reagent (Kovacs reagent), the media changes and the results obtained are interpreted by an automated optical system and a computer.
      
Although the germ cells become eliminated after several media changes, they could interferen with the use of Sertoli cells for critical studies during the first several days of culture.
      
However, this configuration facilitated media changes during culture.
      
Some differences were observed in the response of CF and normal cells to environmental (media) changes.
      
After several media changes, the media were collected and tested for activity.
      
An example for such a horizon would be the ice underside where the media changes from ice to water or the snow-ice interface.
      
Besides the mentioned flexibility the use of New Media changes the processes of learning, too.
      
Cultures were maintained for 6 d with fresh media changes every other day.
      
Cells were grown for 7 days with culture media changes every 48 hours and then characterized as described in the online data supplement.
      
Cultures were maintained with weekly half-media changes, and the number of viable cells present at each week was determined using flow cytometry.
      
Cells were grown for 4 to 7 days with media changes every other day.
      
Control cells without agents were cultured using the same conditions with comparable media changes.
      
Care was taken to avoid disrupting the wells, and they were inspected carefully before and after media changes to ensure spheroids were not lost.
      
For the entire 9-day period, no media changes were performed.
      
Flt-3L was added for all media changes after day 5.
      
In the case of endothelial cells in particular, shear stress during media changes should probably be minimized as much as possible.
      
Thalidomide 1 mg/ml or vehicle was added daily and complete media changes were performed every 48 h.
      
Thus, the lack of effect was not due to a fall of glucose levels by the end of the culture period between media changes.
      
They thought about how the mobile media changes the rules advertising.
      
 

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