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fluorescent signal
The uncoupling agent, CCCP, and the Ca2+-chelator, EGTA, induced marked decreases in the fluorescent signal in cells from both species.
      
Cytochalasin D caused fragmentation of the actin bundles and irregular distribution of the fluorescent signal.
      
Unmodified BOXTO showed no inhibitory effects on real-time PCR, while BOXTO-PRO showed complete inhibition, Sufficient fluorescent signal was acquired when 0.5-1.0 μM BOXTO was used with RotorGene and iCycler platforms.
      
Fluorescent signal suppression was ameliorated by sample dilution or by performing the assay above the pI of secreted IgG.
      
The primary effect of butyrate (a histone deacetylase inhibitor) post-treatment was an increased intensity of the fluorescent signal of green fluorescent protein, as measured by flow cytometry (1.0 to 4.2-fold, depending on the cell line).
      
They combine the sensitivity of conventional PCR with the generation of a specific fluorescent signal providing real-time analysis of the reaction kinetics and allowing quantification of specific DNA targets.
      
The fluorescent signal eliminates the requirement for post-amplification processing steps, such as gel electrophoresis and ethidium bromide staining.
      
The fluorescent signal is proportional to polymer weight concentration and is insensitive to filament length distribution.
      
The Danz fluorescent signal was used to estimate the affinity constants of ETnC-Danz for Ca2+ and also for Mg2+ (assuming a competitive behaviour between these two metal ions).
      
The fluorescent signal of all three dyes was sufficiently intense for localization of the compounds in cells by means of CLSM.
      
In this assay, a fluorescent signal is generated by internalized enzyme in intact cells and not by membrane-bound or extracellular enzyme.
      
The main fluorescent signal of the GFP tagged with either a wild-type Fht1p or mutants which preserve their flocculation function was detected in the nucleus, whereas signals of functionless mutants were dispersed to the cytoplasm.
      
Epifluorescent microscopy was successfully used for the detection of fluorescent signal emitted by the probes in pure and mixed culture samples.
      
For CK-IAF without docked substrates, the time derivative of the initial loss of the fluorescent signal within the M-band equalled -3.26 at the fifth second and the decrease reached 82% by the 67th second.
      
Yet no fluorescent signal is detected when the NSOM tip is located on top of the nanoparticle array.
      
You will not be able to see the fluorescent signal very well if the visible light is left on.
      
You will not be able to see the fluorescent signal if the visible light is left on.
      
Approaches allowing direct induction of the fluorescent signal in GFP-like proteins have also appeared.
      
After 30 minutes the fluorescent signal of predigested DQ-OVA disappeared completely.
      
Amplification was monitored using 5 nuclease probes to generate a fluorescent signal detected with a modified microarray scanner.
      
 

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