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For 'Passe Crassane', shoot buds were regenerated (day 130) on a half-strength MS medium with 0.1 mg/l IBA, 0.5 mg/l BAP, 50 mg/l CEH and 20 mg/l Ca-panthotenate.
      
Shoots were rooted using 2 mg/l IBA and normal plants were re-established.
      
Roots were induced when the resulting shoots were placed on MS medium with 1.0 mg/l IBA.
      
Rooting was induced in medium with reduced salt concentration containing 2 mg/l IBA.
      
After treating these somatic embryos (1-3 mm) in distilled water for a week, 30-40% of them germinated normally and grew into plantlets 20-30 d after transplanting on 1/2 MS medium containing 1 mg/l IBA, 1 mg/l GA3 and 1% sucrose.
      
MS basal medium supplemented with 0.5 mg/l IBA and LS vitamins was found best to produce healthy plantlets in all the genotypes.
      
The highest percentage of embryogenic cultures occurred on the medium containing 0.5 mg/l IBA or 1.0 mg/l BA and 0.5 mg/l IBA.
      
MS medium supplemented with B5 vitamins, 0.125 mg/l IBA and 2 mg/l BAP was found suitable for embryo maturation.
      
Embryogenic lines of Prunus subhirtella autumno rosa were established on a modified MS medium supplemented with 1 mg/l NAA, 0.06 mg/l IBA and 0.04 mg/l BA from petioles of axenically grown shoots of adult origin.
      
Root formation was optimal on medium consisting of full strength MS basal macro elements and vitamins, half strength micro elements, 1% sucrose and supplemented with 0.3 mg/l IBA.
      
Optimal embryo germination and plantlet development was achieved on MS medium with 0.2 mg/l BAP plus 0.1 mg/l IBA.
      
The conversion frequency of cotyledonary embryos was 53.3% upon culture on MS medium containing 0.1 mg/l ABA for 7 days followed by transfer to MS medium supplemented with 0.1 mg/l IBA and 0.2 mg/l BA.
      
Auxin (0.05 mg/l IBA) in combination with cytokinin enhanced adventitious bud formation, with the highest frequency, 40%, at 0.05 mg/l IBA and 5 mg/l BAP.
      
After rooting on basal medium with 0.5?mg/l or 1?mg/l IBA the plants were transferred to soil and showed normal growth and fertility compared to the seed-grown plants.
      
PLB clusters regenerated on MS medium supplemented with 2?mg/l 6-BA, 0.1?mg/l IBA, and 0.1?mg/l GA3.
      
Rooting was most successful on medium supplemented with 6 μg/l IBA alone on which 93.3% of the shoots formed roots.
      
Hybrid plants were successfully obtained by culturing the embryos on B5 medium supplemented with 0.1 mg/l IBA followed by culture on B5 medium supplemented with 0.1 mg/l TBA plus 0.25 mg/l 2-iP.
      
The shoots rooted best on MS medium supplemented with 2.0 mg/L IBA.
      
Up to 98% rooting was achieved on 1/4 MS with 2 mg/l IBA.
      
The best medium for shoot regeneration was that of Murashige and Skoog at 1/4 strength with 3% sucrose (1/4 MS), supplemented with 0.1 mg/l IBA and 0.5 mg/l BAP.
      
 

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