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enzymes
INHIBITORS OF FILARIAL GAMMA-GLUTAMYL CYCLE ENZYMES AS POSSIBLE MACROFILARICIDAL AGENTS
      
The sensitivity of two γ-glutamyl cycle enzymes i.e.
      
Using the de novo design approach, several trisubstituted thiazole analogs were generated as potential inhibitors of these enzymes.
      
euphratica rapidly activated antioxidant enzymes after the onset of salt stress, which might reduce the accumulation of reactive oxygen species and the subsequent acceleration of lipid peroxide.
      
Thus, the antidotal enzymes and digestive enzymes in the midgut of the larvae were inhibited.
      
Sense and antisense expression vectors of PtAP3 were constructed by PCR and restriction enzymes digestion identification, and transformed into tobacco (Nicotiana tabacum) by Agrobacterium GV3101 and LBA4404.
      
The components of insect toxins are very complicated, and hymenopteran venoms contain alkanes, alcohols, aldehydes, ketones, organic acids, esters, lactones, proteins, polypeptides, enzymes, amines and other compounds.
      
Then, the fragments containing the mutation site was obtained from pCR2.1 using restriction enzymes digestion and inserted into the same restriction site of pIRES2-EGFP-KCNQ1.
      
The specific activities of the enzymes involved in secondary metabolism, such as methylmalonyl-coenzyme A mutase (MCM) and methylmalonyl-coenzyme A transcarboxylase (MCT), were detected as well.
      
Iron, which could help phytoplankton grow rapidly, is a micronutrient essential to the synthesis of enzymes required for photosynthesis in the oceanic environment.
      
Compared to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (final concentration) every 24 h produced more enzymes.
      
The average size of the plasmid was finally determined to be 40.2 Kb using selectively three kinds of restricted inscribed enzymes (HindIII, BamHI, and XholI) for single cutting and double cutting the plasmid pTHSL-1, respectively.
      
Increases in the activities of nitrile hydratase and amidase and resistance of these enzymes to ammonium were observed in mutant ofR.
      
In the second scenario, the exploded willow was initially adjusted by alkali to pH 4.5 and then hydrolyzed directly by the added enzymes over 24 h.
      
The enzymes exhibited optimum activity at pH 6.5 and 6.0, respectively (medium, phosphate buffer).
      
Extracellular enzymes-a chitinase and a proteinase with molecular weights 22 and 32 kDa, respectively-were isolated fromStreptomyces kurssanovii cells.
      
After purification on modified regenerated chitin, the enzymes were virtually homogeneous according to denaturing PAGE.
      
Weakly adsorbed enzymes displayed a hydrolysis efficiency of not less than 80% per IFPU enzyme consumed in extended hydrolysis of pretreated willow as compared to the original enzyme mixture.
      
The enzymes were purified by affinity chromatography on an α-cyclodextrin polymer and gel filtration on Biogel P-150 and proved to be electrophoretically homogeneous.
      
The enzymes were purified by Ultrafiltration, ion exchange chromatography, and exclusion HPLC.
      
 

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