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imidacloprid
Determination of the Insecticide Imidacloprid in Potato (Solanum tuberosum L.) and Onion (Allium cepa) by High-Performance Liqui
      
A high-performance liquid chromatography (HPLC) method has been proposed for the determination of imidacloprid residues in fortified potato (Solanum tuberosum L.) and onion (Allium cepa) and in treated potato and onion samples.
      
Sample preparation consisted of dichloromethane extraction of imidacloprid from the plant material, followed by purification of the obtained onion extract on an LC-Florisil disposable cartridge.
      
The HPLC-diode-array-detection (DAD) method has been developed on reversed phase for separation of imidacloprid with isocratic elution with a mixture of 0.01 M phosphate buffer (pH 3.15) and acetonitrile (75 : 25, v/v).
      
Spectral data obtained with DAD allow the identification of imidacloprid residue.
      
Imidacloprid was recovered from fortified samples in a range of 94-99%.
      
The within-day RSD of repeatability of the retention time of imidacloprid standard solution was less than 0.1% and of the obtained peak area less than 5%.
      
Evaluation of genotoxicity of combined soil pollution by cadmium and imidacloprid
      
Cadmium (Cd) is one of the important pollutants of soil and the genotoxicity of Cd-contaminated soil was studied in combination with imidacloprid.
      
The single cell gel electrophoresis or comet assay was used to quantify DNA strand breaks as a measure of DNA damage induced by Cd and imidacloprid contamination in soil.
      
The soil was artificially contaminated by Cd (0.0, 0.2, 0.5, 1.0, 2.0 mg· kg-1 dry soil) or Cd (0.0, 0.2, 0.5, 1.0, 2.0 mg · kg-1 dry soil) and imidacloprid (0.5 mg · kg-1 dry soil).
      
However, the addition of imidacloprid further increased the DNA damage.
      
These data confirmed the genotoxic effect of Cd to plants, and that the combined pollution with imidacloprid can enhance the genotoxicity of Cd.
      
Imidacloprid is a newly introduced broad-spectrum chloronicotinyl insecticide and will find its way in agricultural production, particularly in Asia.
      
However, information on the fate of imidacloprid in crop plants is lacking.
      
Imidacloprid was rapidly dissipated in processed tea following first order reaction kinetics at all application rates and had half-lives of 0.91~1.16 d with the residue in tea liquor found to be below detectable limit on 3rd day samples.
      
The study revealed that imidacloprid is safe for human consumption and will not pose any residual toxicity problem.
      
Use of High-Performance Liquid Chromatography-UV and Gas Chromatography-Mass Spectrometry for Determination of the Imidacloprid
      
Imidacloprid is a new insecticide with a wide range of action.
      
Imidacloprid can often be found in the environment, not as a simple molecule but as a group of degradation products.
      
 

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