助手标题
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
共[3714]条 当前为第1条到20条[由于搜索限制,当前仅支持显示前5页数据]
 

相关语句
flap
Application of three-dimensional digitalized reconstruction of latissimus dorsi myocutaneous flap
      
The aim of this study was to reconstruct the normal structures of thoracodorsal artery in 3D images and to establish the digitized visible models of latissimus dorsi myocutaneous (LDM) flap.
      
Three-dimensional computerized reconstructions of LDM flap structures were conducted from these datasets by using Amira 3.1 (TGS) software respectively.
      
The 3D reconstructed visible models established from these datasets perfectly displayed the anatomic characteristics of LDM flap.
      
Affinity Labeling of Flap-endonuclease FEN-1 by Photoreactive DNAs
      
Eukaryotic flap-endonuclease (FEN-1) is 42-kD single-subunit structure-specific nuclease that cleaves 5"-flap strands of the branched DNA structure and possesses 5"-exonuclease activity.
      
Formation of a structure with the 5"-flap containing the photoreactive group results in decrease of the level of protein labeling caused by cleavage of the photoreactive group due to FEN-1 endonuclease activity.
      
Interaction of Flap Endonuclease-1 and Replication Protein A with Photoreactive Intermediates of DNA Repair
      
The formation of complexes of flap endonuclease-1 (FEN-1) with flapped DNA was shown by photoaffinity modification and gel retardation assays.
      
The substrate properties of the flapped structures with different flap lengths were studied in the reaction of endonuclease cleavage catalyzed by FEN-1.
      
A significant inhibition of cleavage was observed when the flap length was sufficient for effective RPA binding, while for structures with short single-stranded part the efficiency of cleavage was independent of the presence of RPA.
      
FEN-1 and RPA were modified by photoaffinity labeling using flap structures with single-stranded parts consisting of 8 and 21 nucleotides.
      
Products of DNA photoattachment to FEN-1 were observed in both cases, while the covalent adducts with RPA were obtained only with the 21-nucleotide-long flap.
      
Use of Modified Flap Structures for Study of Base Excision Repair Proteins
      
When we introduced the second modification in the flap-forming oligonucleotide, the cleavage rate decreased significantly.
      
Efficiency of strand-displacement DNA synthesis catalyzed by Pol β was shown to be higher for flap structures containing non-nucleotide linkers.
      
The chimeric structures were processed by the 3'-exonuclease activity of APE1 with efficiency lower than that for a normal flap structure.
      
The photoreactive DNA duplexes-3'-recessed DNA, nicked DNA, and flap structures containing natural and chimeric oligonucleotides-were used for photoaffinity labeling of BER proteins.
      
Some of DNA duplexes used in this work can be considered as short-patch (DNA with the 5'-phosphate group in the nick/gap) or long-patch (DNA containing 5'-sugar phosphate or 5'-flap) base excision repair (BER) intermediates.
      
Gas-dynamic engines, a split balancing flap, and an air brake are used as mounting devices of control.
      
 

首页上一页12345下一页尾页 

 
CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社