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mesangial cells
Cloning and high-level expression of plasminogen activator inhibitor-1 cDNA derived from human glomerular mesangial cells
      
The transcriptional regulation of SCL was studied in vitro in rat mesangial cells (MC).
      
It was reported heparin could inhibit mesangial cells proliferation in vitro.
      
The main aim of this study is to explore whether heparin inhibits proliferation of mesangial cells grown in high glucose concentration and to measure the effect of heparin on matrix metalloproteinases (MMPs) expression in mesangial cells.
      
A metabolic (WST-1) assay was used to measure mesangial cell proliferation and Western blot analysis was used to measure MMPs expression of mesangial cells.
      
In the presence of heparin, mesangial cells proliferation was inhibited, which was more obvious at high glucose concentrations than at low glucose concentrations.
      
In high glucose, with heparin concentration of 50, 100, 200 and 400 μg/mL, the mesangial cells showed a 0.61 fold, 0.52 fold, 0.52 fold and 0.41 fold reductions in cell number compared to cells grown without heparin.
      
In Western blot analysis, MMP1, MMP2, MMP3 and MMP9 was expressed by mesangial cells expressed in both high and low glucose concentrations, which was more prominent in high glucose medium.
      
3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the ge
      
3 (N-3) recipe on nitric oxide (NO), nitric oxide synthase (NOS) secreted by cultured mesangial cells (MC) and its gene expression of the inducible nitric oxide synthase (iNOS).Methods; The drug (nephritis No.
      
3)-containing serum was prepared with serum pharmacological technique, and then was applied to react on mesangial cells cultured in fetal calf serum (FCS) and cells cultured in FCS plus lipopolysaccharide.
      
Phenotypic modulation of mesangial cells in diabetic rats and effect of tujian mixture on it
      
ROS mimic the stimulatory effects of HG and upregulate transforming growth factor-a1, plasminogen activator inhibitor-1, and extracellular matrix (ECM) proteins by glomerular mesangial cells, thus leading to mesangial expansion.
      
Recent studies suggest that nicotine has powerful mitogenic effects and induces extracellular matrix production in human mesangial cells via reactive oxygen species generation.
      
Functional renin receptors in renal mesangial cells
      
Recently, a new renin receptor was cloned in renal mesangial cells.
      
Unexpectedly, the receptor induces angiotensin-independent cellular effects in renal mesangial cells, suggesting that renin has novel receptor-mediated actions that could play a role in renal fibrosis.
      
Effects of cyclosporin a on proliferation of cultured rat mesangial cells
      
The proliferation of mesangial cells on cyclosporin (CsA) test medium was studied by MTT assay and TNF-α in cultured supernatant was examined by using ELISA.
      
The results showed that cyclosporin A significantly inhibited the proliferation of mesangial cells at the concentration between 0.
      
 

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