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Biomimetic strengthening polylactide scaffold materials for bone tissue engineering
      
The novel composite mainly consists of nano-hydroxyapatite (n-HA), which is the main inorganic content in natural bone tissue for the PLA.
      
The crystal degree of the n-HA in the composite is low and the crystal size is very small, which is similar to that of natural bone.
      
The biomimetic three-dimensional porous composite can serve as a kind of excellent scaffold material for bone tissue engineering because of its microstructure and properties.
      
The effects of hematopoietic stem/progenitor cells (HSPCs) expanded in the two step coculture with human bone marrow mesenchymal stem cells (hMSCs) on the hematopoietic reconstruction of irradiated NOD/SCID mice were studied.
      
Effects of malondialdehyde on growth and proliferation of human bone marrow mesenchymal stem cells in vitro
      
Malondialdehyde (MDA) is a well known inducer of carbonyl stress in a variety of human cells, however, its effects on human bone marrow mesenchymal stem cells (hMSCs) have not been documented.
      
In this study, murine mononuclear cells (MNCs) were isolated from bone marrow and cultured in suspension, and then Lin-CD117+ HSCs were isolated by immunomagnetic beads.
      
Cytokines mRNA in bone marrow-derived dendritic cells in asthmatic mouse
      
By inducing and amplifying dendritic cells (DCs) derived from the bone marrow of asthma murine in vitro, cytokines mRNA were expressed, and the functions of DCs were investigated.
      
Cells isolated from murine bone marrow have been cultured with rmGM-CSF and rmIL-4, and the expression of cytokines mRNA was determined by ribonuclease protection assay combined with multi-probe templates.
      
Large numbers of DCs have been obtained from bone marrow, and they expressed interleukin-13 (IL-13), interleukin-9 (IL-9), and interleukin-3 (IL-3) mRNA.
      
Measurement of bone alkaline phosphatase and relative study with osteosarcoma
      
The objective of this paper is to explore the value of bone alkaline phosphatase (BALP) for diagnosing osteosarcoma, evaluating the effect of the chemotherapy, judging the prognosis and supervising the relapse and metastasis.
      
We also studied the feasibility of improving tissue vascularization of the engineered bone by using small intestine submucosa (SIS) as the scaffold.
      
Bone mesenchymal stem cells (BMSCs) were isolated by gradient centrifugation method.
      
Bone mesenchymal stem cells were seeded in the SIS, and the scaffold-cell constructs were cultured in vitro for 2 weeks.
      
The scaffold-cell constructs formed a lot of bone and blood vessels in vivo.
      
The SIS can be used as a scaffold for constructing tissue-engineered bone as it can improve the formation of bone and vessels in vivo.
      
Pluripotent stem cells exhibiting similar characteristics can be isolated from human fetal bone marrow, heart, liver, muscle, lu
      
 

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