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solid enzyme
Esterificaton was found to take place in both low water systems containing solid enzyme in hexane and in biphasic aqueous enzyme solution/hexane systems.
      
In the solid enzyme system, the enzyme adsorbed the water produced, thus delaying the appearance of a discrete aqueous phase.
      
Reaction velocities were quite similar for the solid enzyme/hexane system and for the biphasic aqueous enzyme solution/hexane system.
      
For two different preparations of cytochrome oxidase, this method yields an average value ofEa=0.27 ev, which agrees well with direct conductivity measurements on dry solid enzyme, which provide an average value ofEa=0.26 ev.
      
Bioimprinted-immobilized enzyme preparations gave 4-6.2-fold activity enhancements compared to solid enzyme preparations supplied by manufacturers.
      
In the latter case the water present is located mostly on the solid enzyme particles.
      
 

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