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genes
Simultaneous introduction of multiple genes into plants is a critical step in plant genetic engineering to manipulate multiple functional genes in metabolic engineering and trait stacking.
      
It is important to construct a bidirectional promoter for transforming two or more genes into plants simultaneously.
      
The transient expression of the gusA and gfp genes were detected by histochemical staining for GUS and by fluorescence microscopy for GFP.
      
At present, this procedure can be used to evaluate the genetic effect of individual major genes (up to two to three major genes), the collective genetic effect of polygene, and their heritability value.
      
Molecular mapping of two semidwarf genes in an indica rice variety Aitaiyin3 (Oryza sativa L.)
      
Genetic analysis established that Aitaiyin3, a dwarf rice variety derived from a semidwarf cultivar Taiyin1, carries two recessive semidwarf genes.
      
By using simple sequence repeat (SSR) markers, we mapped the two semidwarf genes, sd-1 and sd-t2 on chromosomes 1 and 4, respectively.
      
Two mutant viruses, B9F and Li6A were isolated, and the sites of transposon insertion were determined to be within the coding regions of the 94k and p10 genes, respectively.
      
Both genes were determined to be nonessential in viral replication and infection.
      
This technique will be very useful in the functional study of baculovirus genes.
      
As no male sterile genes have been reported in this region, ms157 could be a novel gene related to fertility.
      
Zinc finger genes are some of the largest gene families in humans, and Zinc finger proteins play an essential role in altering gene expression by acting as transcription factors.
      
Studies on the statistical characteristics of EID show that there were 103, 848 genes, 478,484 introns, and 582,332 exons, with an average of 4.61 introns and 5.61 exons per gene.
      
Cloning and functional identification of stress-resistant BeDREB genes from Bermuda grass
      
Two DREB-like genes from Bermuda grass that are induced by low-temperature or high-salt stresses were cloned using RT-PCR and RACE methods, and were named BeDREB1 and BeDREB2, respectively (GenBank accession No: AY462117 and AY462118).
      
Interestingly, these two genes isolated from Bermuda grass induced either by low-temperature stress or high-salt stress shared 97.8% homology.
      
RT-PCR showed that BeDREB1 and BeDREB2 genes could be greatly induced by low-temperature and high-salt stresses, respectively.
      
In conclusion, all results indicate that BeDREB1 and BeDREB2 genes isolated from treated Bermuda grass are new members of the DREB transcription activator family, which may play very important roles in signal transduction related to stresses.
      
Correlation between the distribution pattern of virulence genes and virulence of Aeromonas hydrophila strains
      
These genes were investigated using polymerase chain reaction (PCR) with specific primers for each gene.
      
 

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