助手标题
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
共[5]页 共[98]条 当前为第1条到20条
 

相关语句
target rna
The probes hybridize to target RNA and the liposome-target-bead complex is subsequently captured on a magnet.
      
By binding to the target RNA, protein production is interrupted and target protein levels decease.
      
This strategy allowed the detection of as little as 2.5 pg target RNA from pure culture and 500 cells from inoculated meat homogenate, even in the presence of other contaminating bacteria.
      
Oligonucleotide probes enzymatically labelled at the 3'-end with biotin have been used successfully to detect target RNA and DNA in combination with in situ hybridisation.
      
Detection of target RNA by in situ hybridization (ISH) in the classic and confocal fluorescence microscope was performed using strand-specific single-stranded RNA probes labeled directly with the fluorochromes fluorescein isothiocyanate or Texas red.
      
The steady-state concentrations of ribozyme and target RNA were shown to be similar in the resulting single transformants.
      
Ribozymes of the hammerhead class can be designed to cleave a target RNA in a sequence-specific manner and can potentially be used to specifically modulate gene activity.
      
Transcribed messenger RNA encoding the green fluorescent protein (GFP) that includes a target RNA sequence is sensitive to cleavage chemistry mediated by metal derivatives of GGH(G)xTRQARRNRR RRWRERQR (x?=?0, 1, 2, 4, 6).
      
However, target inactivation was also observed in experiments with metal-free peptide, consistent with recruitment of intracellular metal ion by the peptide following cellular uptake, with subsequent cleavage of the RRE target RNA.
      
These siRNAs form a complex with helicase and nuclease enzymes known as "RNA-induced silencing complex" (RISC) that leads to target RNA degradation.
      
This ribozyme can target a cancer-specific transcript and then replace the RNA with new transcripts, resulting in induction of the transgene activity selectively in cancer cells that express the target RNA.
      
The RNA replacement occurs by trans-splicing reaction with high fidelity with the target RNA.
      
Detection of differentially expressed gelatinase A in metastatic and non-metastatic subpopulations of tumor cells by target RNA
      
AmpliDet RNA consists of nucleic acid sequence-based amplification (NASBA) of the target RNA with specific primers and simultaneous real-time detection of the amplification products with molecular beacons.
      
Following its assembly, the RISC guides the RNA degradation machinery to the target RNAs and cleaves the cognate target RNA in a sequence-specific, siRNA-dependent manner.
      
Pharmacophore superimposition and docking into the target RNA suggest perfect matching between the template molecule and the designed compound.
      
By observing the H5-H6 TOCSY cross peaks of the series of 2H-labeled RNAs complexed with the Sxl RBD1-RBD2, all of the base H5-H6 proton resonances of the target RNA were unambiguously assigned.
      
This indicates the general applicability of the experiments and the possibility to obtain correlations for imino resonances in non-canonical regions of the target RNA.
      
The target protein is inserted into the scaffolding of the GFP, eliminating false-positive artifacts caused by expression of truncated protein variants from internal cryptic ribosome binding sites in the target RNA.
      
The synthetic ribozyme appeared able to correctly cleave in vitro the target RNA.
      
 

首页上一页12345下一页尾页 

 
CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社