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giant panda
The bamboo Fargesia nitida, one of the giant panda's main food sources and the dominant shrub species of the forest understory, is mainly distributed in the dark coniferous belt in western Sichuan and southern Gansu in China.
      
Sequence variation and genetic diversity in the giant panda
      
About 336-444 bp mitochondrial D-loop region and tRNA gene were sequenced for 40 individuals of the giant panda which were collected from Mabian, Meigu, Yuexi, Baoxing, Pingwu, Qingchuan, Nanping and Baishuijiang, respectively.
      
The results showed that the giant panda has low genetic variations, and that there is no notable genetic isolation among geographical populations.
      
The ancestor of the living giant panda population perhaps appeared in the late Pleistocene, and unfortunately, might have suffered bottleneck attacks.
      
By using PCR cloning techniques, the DNA sequences of the HMG box regions of sixSox genes (pSox) and the zinc finger domains of twoZfz genes (pZfx) in the giant panda were identified.
      
ThepSox andpZfx genes of the giant panda were highly homologous to the corresponding genes in mammals and revealed close substitution rates to those in the primates.
      
The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of
      
The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured and used as nucleus donor for the construction of intenpecies embryos by transferring them into enucleated rabbit eggs.
      
In addition, giant panda mitochondrial DNA (mtDNA) was shown to exist in the intenpecies reconstructed blastocyst.
      
Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda
      
Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified.
      
DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda.
      
Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.
      
Evaluation of habitat fragmentation of giant panda (Ailuropoda melanoleuca) on the north slopes of Daxiangling Mountains, Sichua
      
In this study, we focus on the effect of human activities on the habitat and population of the giant panda (Ailuropoda melanoleuca) in the north slopes of Daxiangling Mountains, Sichuan province, China.
      
Based on eight months of field investigation in 2004, we found that the distribution of the giant panda population has been limited to remnants of habitat due to human activities such as road construction, mining and hydropower stations.
      
There is 100.60 km2 area suitable for the giant panda (including moderately suitable, suitable and most suitable), which only accounts for 21.33% of the bamboo area.
      
Based on a dispersal model COST, there were 79.94 km2 areas (17.12%) (including moderately suitable, suitable and most suitable) for giant panda utilization in reality.
      
The results of this study can be used to provide basic information to build nature reserve for protecting giant panda in Daxiangling Mountains.
      
 

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