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    (4), The positive expression of p27kipl protein were significantlyassociated with higher spontaneous apoptosis and lower expression of PCNA compared with the negative expression of p27kipl protein (P<0.05).
    (5)、分别以bel一2、p27kiPI蛋白表达阳性和阴性分为两组,检测自发凋亡率Al和PCNA指数PI。 其中p27kiPI蛋白阳性组中Al均数显著高于p27 kipl蛋白阴性组(p<0.05)。
    We detect the expression of Smad4 in BPH, to evaluate the relationship with proliferation and apoptosis rate of prostatic cell and to explore the regulating mechanism of Smad4 to BPH.
    本研究应用流式细胞技术检测了正常人前列腺组织和前列腺增生症病人前列腺组织中细胞的增殖率和凋亡率,并应用Western blotting技术检测了Smad4在前列腺组织中的表达,评价Smad4与前列腺组织细胞增殖率和凋亡率的关系,探讨Smad4对前列腺细胞生长的具体调控机理。
    The expression of Smad4 is negatively correlated with proliferation rate of prostatic cell( P <0. 01) and the coefficient was -0. 857.Conclusion
    3、相关性分析:Smad4表达与前列腺增殖率呈负相关(P<0.01),相关系数为一0.857。 Smad4与凋亡率无相关性(P>0.05)。
    3. The expression of Smad4 in BPH is associated with proliferation rate of prostatic cell, but not with apoptosis rates
    3.前列腺中Smad4表达与细胞增殖率有关,与细胞凋亡率无关。
    Rates of cell proliferation and apoptosis in the ventral prostate of intact rats were 8. 93 +2. 99% and 8. 98 +3. 57% , respectively. Those in castrated rats were 5.98 +2. 97% and 12.42 +4. 05% , respectively.
    流式细胞技术检测细胞增殖率和凋亡率:正常大鼠前列腺组织中细胞的增殖率为8.93土2.99%,凋亡率为8.98士3.57%,去势术后前列腺细胞增殖率为5.98*2.97%,凋亡率为12.42士4.05%。
    Correlation analysis: The expression of Smad4 positively correlated with the rate of apoptosis (r =0. 797 ,P <0.05) , but it had no correlation with the rate of proliferation.
    相关性分析:大鼠前列腺组织中Smad4表达与凋亡率正相关(r= 0.797,P<0.05),与增殖率无相关性。
    Exposed 1.25mg/ml OMT for 72h, they were 54.52%, 44.42%,1.06% , respectively and apoptosis cells were 5.37%;
    1.25mg/ml苦参素作用72h后分别是54.52%, 44.42%,1.06%,凋亡率是5.37%;
    5. detecting the activity of the sialidase. of three kinds of cells, that of pECF-C1-hmSD is significantly up-regulated (p<0.05)。
    7.流式细胞仪检测发现,相同剂量丁酸钠对PC-3细胞,pEGFP-C1细胞,pEGFP-C1-hmSD细胞凋亡率影响不同,pEGFP-C1-hmSD的凋亡率低于前者(p<0.05),显示了hmSD明显的凋亡抵抗作用。
    Methods:1) Rat mesangial cells were cultrued by fresh serum-free medium 1640 containing 5、10、20、30、40 mM glucose and 30 mM mannitol for 24h、48h、72h and then examined apoptosis ratio by flow cytometry AnnexinV/PI double stains.
    方法:1)大鼠肾小球系膜细胞分别经含5mmol/L、10 mmol/L、20 mmol/L、30 mmol/L、40 mmol/L葡萄糖及含30mmol/L甘露醇的1640培养基培养24h、48h、72h后用流式AnnexinV/PI双染法检测凋亡率
    Our results demonstrate that: 1.Antiprogestin mifepristone could induce apoptosis of both androgen-dependent prostate cancer cell line LNCaPC4 and androgen-independent prostate cancer cell lines LNCaPC4-2、PC3 in vitro, it made no difference of the two type of cell lines.
    PC3细胞株受15uM米非司酮作用24小时细胞凋亡率为:44.52%。 实验结论:1、米非司酮对雄激素依赖型前列腺癌细胞株LNCaPC4及雄激素非依赖型前列腺癌细胞株LNCaPC4-2、PC3均有生长抑制和促进凋亡作用。
    3. using flow cytometry method, the apoptosis rates of 15 uM mifepristone on LNCaPC4-2 cellline were 19.30% and 30.04% respectively at 24h and 48h.
    3、流式细胞仪检测雄激素非依赖型前列腺癌细胞株LNCaPC4-2受15uM米非司酮作用24小时和48小时细胞凋亡率分别为19.30%、30.04%。
    The apoptosis rate of the same concentration of mifepristone on PC3 cell line at 24h was 44.52%.
    PC3细胞株受15uM米非司酮作用24小时细胞凋亡率为:44.52%。
    ⑶The percentage of apoptosis was examined by flow cytometry.
    ⑶流式检测凋亡率;
    ResuIts: The apoptosis ratio of T24 cells on day 5 with 2.5μmol/L、 5μmol/L and 10μmol/L 4-HPR-treated was 12.17%,28.0% and 26.76%, respectively, and the mortality rate were 3.26%,12.35% and 26.66%,respectively.
    结果2.5μmol/l、5μmol/l、10μmol/l的4-HPR作用于T24细胞5天时的凋亡率分别为12.17%、28.00%和26.76%,死亡率分别为3.26%、12.35%和26.66%。
    The positive rate of apoptosis of bladder tumor in postoperative (100%) more high than in preoperative (40%).
    术后肿瘤细胞的凋亡率的阳性率(100%)明显比术前为高(术前40%,12/30)。
    The dual staining flow cytometry analysis showed that the early apoptotic rates for 12, 24 and 36 hours were 0.51%, 1.49%, 2.29% for E-J cells respectively, and 59%, 32.78%, 5.19% for BIU-87 cells respectively. The early apoptotic rates of control group for two cell lines were 0.03% and 0.77% respectively.
    MT-12与两株细胞分别作用12h、24h和36h,流式细胞术检测的E-J细胞早期凋亡率分别为0.51%、1.49%和2.29%,BIU-87细胞早期凋亡率分别为59%、32.78%和5.19%,两株细胞的阴性对照组早期凋亡率分别为0.03%和0.77%。
    ②Annexin-V-FITC/PI assay has been used for detection apoptosis rate of Renca cells after radiation. ③ BALB/c mice were inoculated s.
    ②采用Annexin-V-FITC/PI法流式细胞仪检测体外不同剂量X线照射后Renca细胞凋亡率
    Flow cytometric profiles revealed that Crocin led to the increase of the cell percentage of G0/G1 phase and the cell percentage of apoptosis.
    流式细胞术显示Crocin对细胞周期的影响存在时间依赖性,并呈明显的G0/G1期阻滞现象,细胞凋亡率随Crocin作用时间的延长逐渐增加;
    Results The rate of apoptosis was 9. 2±1. 61% in normal renal tissue and 4.15±1. 93% in renal cell carcinoma.
    结果 正常肾组织的凋亡率为9.2±1.61%,肾痛组织的凋亡率为4.15±1.93%。
    Apoptotic rate was inversely correlated with expression of bcl-2 ( r = -0. 915 5) P <0. 001), and apoptotic rate of the samples in which bcl-2 was not expression in RCC were lower than that of normal ones.
    凋亡率与bcl-2的表达呈负相关(x=—0.9155,P<0.01),在bcl-2不表达的肾癌中,凋亡率也低于正常组织(P<0.001)。
 

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