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fermentation condition
    Study on Antagonistic Mechanism and Fermentation Condition of Trichoderma Koningii
    康氏木霉的拮抗机制及发酵条件的初步研究
    Study on Optimal Fermentation Condition for the Expression of Bioactive Opioid Peptides by Pichia Pastoris
    毕赤酵母基因工程菌表达阿片肽发酵条件的研究
    Breeding of L-serine Producing Strain and Optimization of Fermentation Condition
    L-丝氨酸高产菌株筛选及摇瓶发酵条件优化
    Study on L-Ornithine Fermentation Condition and Analysis of Fermentation Kinetics
    L-鸟氨酸发酵条件优化及其发酵动力学研究
    Study on Astaxanthin-overproducing Strain Breeding, Fermentation Condition Optimization and Metabolic Flux Analysis of Phaffia Rhodozyma
    高产虾青素红法夫酵母的选育、发酵条件优化及代谢通量分析
    After optimizing the fermentation condition the expression level of interferon α2b was estimated as 62.5mg/L.
    经过对摇瓶发酵条件的初步优化,估计干扰素α2b的表达量为62.5mg/L左右。
    (3) The batch fermentation condition of strain TV2564 in shake flask was studied in this dissertation.
    (3)研究了菌株TV2564的摇瓶分批发酵条件
    Under the best fermentation condition, the fermentation experiment in 30 M~3 large scale fermenter was also performed. The fermentation data in 30 M~3 fermenter have proved the practicability of fed-batch fermentation technology. The average yield of natamycin in 30 M~3 fermenter could reach 4. 8g/L, the maximum yield of natamycin in 30 M~3 fermenter could reach 5. 4g/L.
    (4)通过实验室5L发酵罐的调控发酵条件研究和30M~3大型发酵罐生产试验,证明了分批补料发酵工艺的实用性,纳他霉素发酵产量平均达到4.8g/L发酵液,最大到5.4g/L,产物提取率大于60%。
    (2) studies on fermentation condition of H-35;
    (2)诱变株H-35的发酵条件优化;
    The fermentation condition and gradients of the medium have been studied,the results showed the optimum fermentation condition were 48 hr of inoculation age, 96hr of the fermentation time and an initial pH of 6.5-7.2,on that condition.
    对HSLⅠ-306菌株的发酵条件和培养基组成进行了研究,结果发现,HSL Ⅰ-306菌株在菌龄48hr,发酵时间为96hr,发酵培养基pH6.5~7.2时活性最强; 3%~7%盐浓度更适于活性物质的产生;
    [2] The batch fermentation condition of strain TQ2223 in shake flask was studied in this dissertation.
    [2]研究了菌株TQ2223的摇瓶分批发酵条件
    According to the theory of metabolic control fermentation, the dissertation focuses on the breeding, fermentation condition and fermentation kinetics of L-valine producing strain.
    本文以代谢控制发酵理论为指导,对L-缬氨酸产生菌的定向选育、摇瓶发酵条件、5L自控发酵罐发酵动力学分别进行了深入研究。
    Optimum time that the mutants were grown on slant medium were 10d, mature spores were placed in refrigerator for about a week, 10% of inoculum size and 40ml medium in 250ml flask were proved to be the best fermentation condition.
    成熟的孢子斜面在4℃冰箱中放置一周左右,发酵结果最好; 以10%接种量,250mL摇瓶中装量40mL为最佳发酵条件
    Therefore, the obtained mutant is a stable strain. Studies were carried out on the fermentation condition through Single Factor design and Orthogonal Test design, which showed that the optimum fermentation condition was culture temperature 30 ℃, initial pH7.0, rotate speed 180r/min, inoculum age 18h, inoculum size 10%, medium volumes 30ml/250ml.
    运用单因素实验和正交实验设计对突变株HY78产谷胱甘肽的发酵条件进行了研究,研究表明该突变株产谷胱甘肽的最佳发酵条件为培养温度30℃、初始pH7.0、摇床转速180r/min、接种龄18h、接种量10%,摇瓶装液量30ml/250ml;
    Different strategies (different volume of inoculation, different initial pH, different carbon sources, different medium concentration ) was applied to optimized the S78-pVT102U/a -lip fermentation condition, the optimum conditions as follow: The optimum culture medium composition was 3% yeast extraction, 3% tryptone, 2% sucrose;
    从移种量、培养基pH、碳源及培养基组成等方面对S78-pVT102U/α-lip进行初步的发酵条件优化,结果表明:适宜的培养基组成为3%酵母抽提物、3%胰化蛋白胨、2%蔗糖;
    (2) The batch fermentation condition of strain TC-21 in shake flask was studied in this dissertation.
    (2)研究了菌株TC-21的摇瓶分批发酵条件
    The optimal fermentation condition of KA-91 in shake flask was established: fermentation medium(g/L): glucose 22, beef extract 5, yeast 4;
    4、确立了KA-91生产氢化可的松的适宜摇瓶分批发酵条件:发酵培养基为葡萄糖22g/L,牛肉膏5g/L,酵母膏4g/L;
    [2]the batch fermentation condition of strain TQ2223 in shake flask was studied.
    [2]研究了菌株TQ2223摇瓶发酵条件
    The fermentation condition is 8% sucrose, 1.6% peptone, 0.2% citric acid, 0.2% Na2HPO4, 0.3% KH2PO4, 0.03% MgSO4, pH 6.8, 30, 7% inoculation amount, agitating culture at 200r/min for 5 days. The fermentation product of Acetobacter xylinuml.
    经优化后的木醋杆菌产细菌纤维素的发酵条件为:蔗糖8%,蛋白胨1.6%,柠檬酸0.2%,Na2HPO_40.2%,KH_2PO_4 0.3%,MgSO_4 0.03%,pH6.8,30℃,接种量7%,振荡培养5天,摇床转速为200r/min。
    The yield was steadiness at day 5, but viscosity still enlargedcontinually probably because of water evaporation. The optimal fermentation condition was 30h bacterial age, 5% inoculating amount, no more than 20% load, initial pH 8.0, 30℃/180rpm and five days growth.
    最后确定采用30小时菌龄,5%接种量,20%装液量为最高,初始pH值为8.0,30℃/180rpm振荡培养5天为最佳发酵条件
 

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