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fermentation condition
    The optimum fermentation condition which can produce the most effective fermental filtrate was 72 hour for rotational culture time,pH 8.0 of medium,30-34℃,rotational velocity is 150r/min.
    并对其发酵条件进行了研究,结果发现,S-159-05菌株在发酵时间为72h,发酵培养基pH8.0,培养温度30-34℃,摇床转速150r/min时更适于活性物质的产生。
    And the detection of Monacolin K and fermentation condition were studied.
    本实验对高产Monacolin K的菌株进行了筛选,并对Monacolin K的检测条件,红曲霉M32菌株的发酵条件进行了研究。
    Both accuracy and precision are better than that of paper chromatography. (2) One inosine high-producing strain TX-3(Ade+Thi+8-AG +SG) was derived from Bacillus subtilis TY-21 with DES treatment, which could produce inosine 12.41 g/L under the fermentation condition without being optimized.
    2.以枯草芽孢杆菌TY-21为出发菌株,通过硫酸二乙酯(DES)诱变定向选育出一株肌苷产生菌TX-3,其遗传标记为腺嘌呤缺陷、硫胺素缺陷、8-氮鸟嘌呤抗性和磺胺胍抗性(Ade~-+Thi~-+8-AG~r+SG~r)。 在未经优化的摇瓶发酵条件下可产肌苷12.41g/L。
    3. Improved expression level of recombinant neutral protease by P. pastoris was achieved by optimization both the growth phase and induction phase culture conditions. The results indicated that the optimum fermentation condition of the growth phase is as follows: 2% glycerol, 2.5% (NHj) 2SO4 0.00004%Biotin, 10%lmol/L phosphate buffer, pH=5.5, 240rpm, 3048hr, 10% inoculation.
    3.通过对重组毕赤酵母NL19发酵过程的两个阶段发酵条件的优化实验,得出生长阶段的最佳发酵工艺为:甘油2%,硫酸按2.5%,Biotin 0.00004%,lmo比磷酸缓冲液10%,pH=5.5,在240rpm、30℃条件下培养48hr,接种量为10%。
    The optimum fermentation condition determined by monosyllabic test is : 40ml culture medium per 250ml flask, inoculating amount 10%, rotating speed 180r/min, pH5.75, 30癈 fermenting 48h.
    采用单因素实验法对这株产PQD丝状真菌的摇瓶发酵条件进行了研究,确定了其发酵条件为接种种龄40h,接种量7.5%,摇床转速180r/min,培养温度为30℃,250ml三角瓶装液量40ml,初始pH5.75。
    The higher alcohol concentrations as the criterion, orthogonal experiments were carried out at three different factors and levels, and the result indicated that the best fermentation condition is fermentation temperature 16℃, inoculation concentration 1×107cell/ml and natural juice pH.
    以高级醇的产生量为指标对酵母SYS2000的发酵条件进行了三因素三水平的优化实验,确定最佳的发酵条件为发酵温度:16℃,接种浓度:1×107个/ml,果汁原始pH。
    2. The optimization of the best fermentation condition and mixed fermentation.
    2.最佳发酵条件优化及混合发酵。
    We explored fermentation condition of both YSC-1 and the production of its in vivo electron dense particles. Finally we found an approving technique forfermentation, which is the air flow about 4L/min, the agitating rate 250rpm, and the fermentation time 60h.
    对YSC-1菌体生长及细胞内电子致密颗粒产生的发酵条件进行了摸索,找到了较为理想的发酵条件:通气量4L/min,搅拌速度250 rpm,培养时间为60 h。
    the optimum fermentation condition of strain A11 was also determined,which indicated that antibiotics of strain A11 had strong inhibition activity with the initiative pH of 7.2,100ml/250ml medium volume,28℃ temperature, 140r/min rotatory velocity and fermentation 6 days.
    其适宜的摇瓶发酵条件是:初始pH值7.2、摇瓶装量100ml/250ml三角瓶、发酵温度28℃、摇床转速140r/min、发酵时间6d。
    3. After MTG fermentation condition was determined, fermentation was performedin 7L fermentor.
    3.在发酵条件优化后进行了7 L发酵罐的小试。
    Fermentation at 30℃/24h at 80-90% relative humidity seemed better fermentation condition before drying at 50℃ for 12-16h while preparing Masyaura.
    在制备Masyaura时,30℃/24h、相对湿度80-90%时,看来发酵条件更好,然后50℃干燥12-16h。
    The best fermentation condition is: 150mL fermentation medium in 250mL bottle,vaccinates 10 %, 48 hours in shake bed under 46℃;
    最佳发酵条件为:250mL输液瓶装载150mL发酵培养基,接种10%,在46℃条件下摇床发酵48h;
    Then, the ACE-inhibitory activity of the products was also researched, and the best fermentation condition for producing ACE–inhibitory peptide by Lactobacillus helveticus TS2005 was confirmed after optimizing and the change of ACE–inhibitory peptide was also studied in the course of fermentation.
    然后,对发酵液进行了ACE抑制活性的检测,通过对其发酵条件的优化,确定了瑞士乳杆菌TS2005生产ACE抑制肽的最佳发酵条件,并且研究了发酵过程中发酵液ACE抑制活性的变化,确定了产品最佳收获时间。
    Different factors were investigated such as the ingredients of medium, temperature, pH, inoculating amount and medium capacity on production of bacteriocin in order to optimize the fermentation condition.
    本研究探讨了营养要素(包括培养基组成和不同的起始pH值)和环境要素(包括培养温度、装液量和接种量)对细菌素产生的影响,以求得产生细菌素的最佳发酵条件
    1. The effect of different fermentation condition: The inoculating time 36h, inoculating amount 10%, multiplication time 72h, fermentation fluid pH 6.0, methanol supply 1.5%, amount of liquid in fermentation pot 10% and time of inducement and expression of 120h can highly improve production of phytase. 164416.7U/mL of the phytase was obtained. It was higher 15% than before.
    1.植酸酶基因工程菌(E-22)发酵条件的研究:接种龄36h,接种量10%,增菌时间72h,发酵液pH6.0,甲醇添加量1.5%,装液量10%,诱导表达120h能很好的提高产酶量,测得酶活164416.7U/mL,比优化前产酶提高15%。
    The research results of fermentation condition of S.maltophilia showed that the most chitinase was synthesized when cultured in the medium containing 1.5% colloid chitin and 1.0% yeast extract for 60 hours (220r/min) by single factor optimal method and uniform design method.
    通过单因素优化法和均匀设计法对S.maltophilia发酵条件的研究,确定其产几丁质酶的条件为:在pH为7.0的基础培养基中添加1.0%的酵母膏,0.5%胶体几丁质,30℃摇床180r/min培养60h,几丁质酶的产出最大。
    The best fermentation condition has been established that 18h of age, 10%of inoculums size, 1.5% of soybean oil ,40ml medium in 250ml flask and 37 ℃, 120h of cultivation.
    发酵条件以18小时的种龄、10%的转种量、消泡剂为1.5%豆油、装量40ml的250 ml摇瓶、37℃培养120小时为佳。
    In 5 L automated jar fermentor, the optimal fermentation condition was: the seed was pitched with 6.67% after culturing 18 h. The fermentation temperature was on 37℃then was cooled to 32℃after 27 h. Air volume was 1 L/ (L·min) and was agitation speed was 500 r/min, theβ-glucanase activity was 182.2 u/mL.
    5 L发酵罐发酵条件优化为:装料系数0.6,接种量6.67 %,种龄18 h,起始发酵温度37℃,27 h后降温至32℃,通气量1.0 L/(L·min),搅拌转速500 r/min,β-葡聚糖酶活力在51.75 h达到182.52 u/mL,比恒温发酵提高了28 %;
    After preliminary optimization on fermentation condition the yield reach 547.1u/ml.
    对M80-10-7菌株的发酵条件进行了初步优化,使发酵水平提高到547.1u/ml。
    Fermentation medium formula: (NH_4)_2SO_4 is 3%, the inoculum 10 %, the yeast paste 1.2 %, the C_4H_4O_42 %, the sugar 4 %, the fermentation condition is: Initial pH is 8.4, the 500ml triangle bottled liquid volume is 20ml.
    发酵培养基配方:(NH_4)_2SO_4为3%,接种量10%、酵母膏质量浓度1.2%、富马酸质量浓度2%,蔗糖浓度为4%,发酵条件为:初始pH为8.4,500ml三角瓶装液量为20ml。
 

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