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promoter gene
    A Relative Study between the MMP-1 Promoter Gene Polymorphism and Periodontitis
    MMP-1启动子基因多态性与牙周炎相关性研究
    Isolation of DNA from HSV-2 and Purification of ICP10 Promoter Gene
    单纯疱疹病毒2型DNA提取及ICP10启动子基因获得和纯化
    MMP-3 promoter gene containing the 5A/6A polymorphism was amplified by polymerase chain reaction(PCR). The PCR products were digested by Tthlll I and then were separated by electrophoresis on agarose gel.
    PCR法扩增MMP-3启动子区域,酶切法及琼胶电泳法对MMP-3启动子基因5A/6A多态性进行分析。
    MMP3 promoter gene containing the 5A/6A polymorphism was amplified by polymerase chain reaction(PCR).
    聚合酶链反应( PCR)法扩增 MMP 3启动子区域 ,酶切法及琼胶电泳法对 MMP 3启动子基因 5 A/ 6 A多态性进行分析。
    were found between PBC group and control group. Conclusion The polymorphisms of IL-1RN and IL-6 -174G/C appear to be associated with PBC? and the polymorphisms of IL-1 +3 953 and IL-10 promoter gene are not associated with PBC in a Chinese population.
    结论IL-1RN和IL-6-174基因多态性可能与中国人PBC的易感性相关,而IL-1(+3953)及IL-10启动子基因多态性与之不相关。
    The levels of acetylated histone H3 and p21WAF1/CIP1 were detected by Western blot, the expression of p21WAF1/CIP1 gene was detected by reverse transcription-polymerase chain reaction (RT-PCR), and the level of acetylated histone H3 at the site of p21WAF1/CIP1 promoter gene was examined by chromatin immunoprecipitation assay.
    RT-PCR检测p21WAF1/CIP1基因表达; 染色质免疫沉淀分析p21WAF1/CIP1的启动子基因位点组蛋白H3乙酰化水平;
    CONCLUSION: Curcumin, with epigenetic modificative effects, could enhance the acetylayion of histone H3 at the site of p21WAF1/CIP1 promoter gene, improve transcription of p21WAF1/CIP1 gene, and arrest cell cycle progression of Raji cells.
    结论:姜黄素通过表观遗传修饰作用,调节p21WAF1/CIP1启动子基因位点组蛋白H3乙酰化水平,促进p21WAF1/CIP1基因转录,阻滞Raji细胞周期进程。
    [Objective] To clone the 2 335 bp fragment(-2 285/50) of human midkine promoter gene.
    目的克隆人M idk ine(M K)启动子基因2 335bp片段。
    DNA sequence analysis and homology comparison indicated that the result was to be proved to absent the segment of "tatattgttaacttcttgttgaattaaagcaat"from 621bp upstream transcription start site of 2A11 promoter gene and to share 61% homology with 2A11 promoter (GenBank ID M87659,1993). DNA sequence had been submitted to GenBank,and its GenBank submission number was DQ453963.Binary vector was constructed through fusing 1.3kb 2A11 promoter gene with the GUS gene.
    序列分析表明,克隆到的基因序列2A11启动子转录起始位点上游的621bp处缺失了已报道的番茄2A11启动子基因(GenBankIDM87659,1993)序列中的“tatattgttaacttcttgttgaattaaagcaat”片段,其同源性为61%,登入GenBank,ID号为DQ453963;
    Objective Amplification,purification of ICP10 promoter gene.
    目的用单纯疱疹病毒2型DNA,扩增并纯化得到ICP10启动子基因
    Methods The AdEasy-1 adenoviral vector system was used in this experiment. Several recombinant adenovirus with tumor-targeting E2F-1 promoter were constructed and then the E2F-1 promoter gene was checked by PCR and sequencing.
    方法利用AdEasy-1系统,构建含有E2F-1启动子的重组腺病毒并对重组病毒中E2F-1启动子基因进行PCR及测序鉴定。
    Association between polymorphism of tumor necrosis factor promoter gene and asthma
    肿瘤坏死因子启动子基因多态性与哮喘的相关性
    Agar electrophoresis with lul. See picture 1. 1.2 Amplifying IGFBP-1 promoter gene from genome DNA by PCR.
    1 .2从基因组DNA中PCR扩增IGFBP一1启动子,PCR产物全部上样回收后,取1过电泳,得到420bp目的DNA,与晓nebank X67493的IGFBP一1启动子基因大小相符,见图2。
    [Methods] As a template,the human genome DNA was extracted from healthy human blood firstly. And then,the polymerase chain reaction(PCR) was applied to clone the 2 335 bp fragment of human midkine promoter gene.
    方法自健康人血中提取人类基因组DNA作为模板,聚合酶链式反应(PCR)技术克隆人M K启动子基因2 335bp片段。
    Apair of PCRpri mers were created fromthe ICP10 promoter gene of HSV-2(Sal 5 strain) by DNAStar software andtworestrictionsites(Bgl Ⅱand HindⅢ) wereincluded as part of thepri mers.
    用酚:氯仿提取病毒DNA,用PCR方法获得病毒ICP10启动子基因,并用UNIQ-10柱试剂盒纯化PCR产物。
    Firstly, the pGL3Control vector was reconstructed , the pGL3Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFNβ promoter gene was cloned into the pGL3Enhancer vector and pGLIP21, the Luciferase reporter plasmid with IFNβ promoter was established. The availability of pGLIP21 was verified by NDV ,the inductor of IFNβ, the Luciferase activity was assayed in cells transfected with pGLIP21 by Luminometer.
    首先将pGL3-Control载体进行了改构,除去了SV40启动子基因,获得了pGL3-Enhancer载体,将获得的IFN-β启动子基因连入载体中,构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21,并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性,照度计检测荧光素酶活性增强,从而验证了所构建的重组质粒的有效性。
    AIM: To amplify the Id1 promoter gene region and to construct two luciferase reporter gene vectors containing Id1 promoter regions.
    目的:扩增Id1启动子基因,构建Id1启动子的报告基因载体.
 

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