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dna
    Genetic Heterogeneity and Gene Diagnosis of Autosomal Dominant Polycystic Kidney Disease Using Microsatellite DNA and Single Nucleotide Polymorphisms
    应用微卫星DNA和单核苷酸多态性对常染色体显性多囊肾病进行遗传异质性研究及基因诊断
    A rapid salting out precedure for extracting DNA used in HLA genotyping
    快速盐析法提取DNA在HLA基因分型中的应用
    Objective To detect hypermethylation of p14 gene in plasma DNA from patients with bladder transitional cell carcinoma and explore its potential as a biomarker.
    目的检测膀胱移行细胞癌患者血浆p14基因启动子异常甲基化状态,探讨血浆p14基因甲基化改变作为膀胱移行细胞癌分子生物学标志物的可能。
    16SrRNA gene of ten bacterial species were amplified with PCR,by using human genome DNA、HBV-DNA and candida albicans as comparison;
    用PCR技术扩增实验室保留株10株的16SrRNA基因,以人类基因组DNA、HBV-DNA和白色念珠菌为对照,检测该方法的特异性;
    However both genomic DNA and nuclear matrix DNA showed a 940 bp positive fragment with primersⅠ except NM DNA100. The sequence of 940 bp was identical to the DNA sequence of EGFR gene.
    940 bp PCR(引物Ⅰ)产物出现在基因组DNA和除NMDNA100外其他核基质DNA中。 940bp序列与EGFR基因DNA序列完全一致。
    Cell apoptosis was determined by flow cytometry (FCM), DNA ladder electrophoresis, and fluorescent microscopy with Hoechst33258 staining. The expression of apoptosis-related genes Bcl-2 and Bax were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).
    用流式细胞术、DNA电泳和Hoechst33258荧光染色3种方法检测SC-58125作用下T24细胞的凋亡,同时逆转录聚合酶链反应(RT-PCR)检测细胞凋亡相关基因Bcl-2和Bax基因的变化。
    Results:A 5'-CCGTGCCCCAGCC-3' Insertion/Deletion polymorphism in 234 position of Exon1 of CDH1 gene was detected by DNA sequencing technique. Its genotype was homozygous for 5'-CCGTGCCCCAGCC-3' allele insertion(I/I), homozygous for 5'-CCGTGCCCCAGCC-3' allele deletion(D/D) and heterozygous for 5'-CCGTGCCCCAGCC-3' allele insertion/repeat insertion(I/2I), respectively.
    结果:CDH1基因Exon1非编码区234bp位点插入片断碱基序列为5'-CCGTGCCCCAGCC-3',其插入/缺失多态性基因型分为插入\插入纯合子(I/I)、缺失\缺失纯合子(D/D)和插入\重复插入杂合子(I/2I)三种。
    Firstly,the coding region of C1GALT1C1 was amplified by PCR from genomic DNA in peripheral blood and PCR products were directly sequenced.
    提取研究对象外周血基因组DNA,扩增C1GALT1C1基因的编码区,采用PCR产物直接测序的方法进行突变筛查。
    Objective To identify the strain specific DNA sequences of uropathogenic E. coli 132 by suppression subtractive hybridization(SSH)between uropathogenic E. coli 132 and standard reference strain of MG1655.Methods Using SSH to construct the library which contained the differential genes between uropatho- genic E.
    目的通过抑制性消减杂交(suppression subtractive hybridization,SSH)技术,比较致肾盂肾炎大肠杆菌132(UPEC132)与非致病性E.coli K-12 MG1655之间基因组的差异,筛选UPEC132特有的基因片段。
    Positional cloning of a novel DNA fragment in the region of PKD1
    成人型多囊肾病致病基因PKD1区新DNA片段的定位克隆
    Ganciclovir effect on the DNA content of human bladder carcinoma EJ cells transfected with HyTK gene
    Ganciclovir对转导HyTK基因人膀胱癌EJ细胞DNA含量的影响
    Polymorphism of 4 microsatellite DNA linked to polycystic kidney disease gene 1 in Han nationality in Shanghai
    上海市汉族人群与PKD1基因紧密连锁的微卫星DNA的多态性分析
    Presymptomatic molecular diagnosis of autosomal dominant polycystic kidney disease by genescanning microsatellite DNA
    基因扫描微卫星DNA进行常染色体显性遗传性多囊肾病囊肿前诊断
    Genetic polymorphism of three microsatellite DNA loci tightly linked to polycystic kidney disease gene 2(PKD2) in Shanghai Han population
    上海市汉族人群中与PKD2基因紧密连锁的三个微卫星DNA的多态性研究
    Gene diagnosis of autosomal dominant polycystic kidney disease type 2 using microsatellite DNA tightly linked to polycystic kidney disease gene 2
    用与PKD2紧密连锁的微卫星DNA对2型常染色体显性多囊肾病进行基因诊断
    Androgen responsive element decoy DNA inhibits the promoter of prostate specific antigen and induces apoptosis of LNCaP cells
    雄激素应答元件陷阱DNA抑制PSA基因启动子的作用并诱导LNCaP细胞凋亡(英文)
    CAG Repeats of DNA Polymerase Gamma in Chinese Males and Relationship of Idiopathic Male Infertility to CAG Repeats
    中国生育男性DNA聚合酶γ基因CAG三核苷酸重复及与特发性男性不育的相关性研究
    DNA chip analysis of signal transduction genes in human urinary bladder transitional cell carcinoma
    DNA芯片技术筛选膀胱移行细胞癌信号转导相关基因
    Detection of hypermethylation of p14 gene in plasma DNA from patients with bladder transitional cell carcinoma
    膀胱移行细胞癌患者血浆p14基因异常甲基化检测的意义
    THE POLYMORPHISMS OF MICROSAUELLITE DNA OF PKD_1 IN GUANGXI DONG NATIONALITY POPULATION
    侗族人群成人多囊肾病基因1微卫星DNA多样性分析
 

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