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    3) Blood taken and preservation after centrifugation: 5ml blood were taken from vein in right upper arm, then blood was added in pre-cold test tube treated with anticoagulant and trasylol.
    3)抽血及血样的离心保存:均于右上肢皮下头静脉抽血snd,立即加人预冷,已加人抗凝剂及抑肽酶的试管中,并低温离心,-20℃保存血浆。
    Then I purified and extracted the target fragment by using UNIQ-10 pillar purification kit and cloned the product added A-tail into pGEM-T easy vector by T-A ligation. The JM109 competent cells were transformed with the recombinant plasimid. Consequently,the recombinant plasimid was amplified and extracted from the transformants and tested the inserting of DNA fragment.
    用UNIQ-10 柱式DNA 胶回收试剂盒回收纯化目的片段,通过T-A 连接将已加A 尾的PCR 产物连接到线状克隆载体pGEM-T easy vector,转化JM109 感受态细胞,在含氨苄青霉素的LB 平板上筛选阳性克隆。
    In the present study,one pair of specific primers to NA gene of Nl subtypes were designed to amplied cDNA of Nl by RT-PCR,purified the production of PCR used reagents made by ourself and cloned the production added A-tail into pGEM-T easy vector by T-A ligation.
    用自制的试剂纯化目的片段,通过T-A连接将已加A尾的PCR产物连接到克隆载体PMD 18-T Vector上,转化JM109感受态细胞,在含氨苄青霉素的LB平板(或麦康凯琼脂平板)上筛选阳性克隆,经PCR扩增,进一步确证为目的片段,并对目的片段进行测序。
    Then DNA adducts are purified and separated by thin layer chromatography on polyethylencimine (PEI)-ccllulose sheets, and produced characteristic autoradiographic fingerprints.
    经聚乙烯亚胺(PEI)纤维素薄层纯化、分离和放射自显影,则可确定已加成的碱基,若DNA量已知,则可进行定量分析。
    With the mag-card inserting into the fuelling controller,fuelling begins with the refuel-er picked off,fuelling stops with the refueler hung on when tank is full or filled to the need,later, settlement is carried on,and then the mag-card is returned.
    该系统采用磁卡加油,分为非定量加油和定量加油两种方式,把磁卡插入加油控制器,摘下油枪即可加油,当油箱加满或已加至需要量时,挂枪即停止加油,随即进行结算、退卡。
    It's a challenge and an opportunity for China's agriculture after China's entering WTO.
    我国已加人WTO,这对农业既是挑战又是机遇。
    With certain deep pit project as example, dangerous slide surfaces of all construction steps are gotten using this method. Safe coefficients of 3 kinds of operating modes including not adding nails, already adding nails of this step and not adding nails of this step, are compared.
    以某深基坑工程为例,利用该方法得到了土钉支护各个施工阶段的危险滑动面,比较了不加钉、本步已加钉和本步尚未加钉3种工况下的安全系数.
    Methods: Twenty-two AA patients whose median age was 26 years, including 12 untreated patients and 10 non-responding ones to ever management, were administrated of 5mg anti-CD3 McAb per day for total 10 days.
    方法:22例AA患者中位年龄26岁,其中重型再障(SAA)15例,慢性再障(CAA)7例。 既往未经特殊治疗12例,既往治疗无效的10例中,已加用环孢素(CsA)≥3个月7例。
 

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