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    Seven of the 21 cDNA being similar to tick genes, 2 with ACGCGGGG and 5 with Poly(A), are ESTs;
    在与蜱已知基因有相似性的21个片段中,7片段被认定为EST,其中5个具有Poly(A),2个具有ACGCGGGG;
    HaB1 and Ha7.7 are two ESTs selected from the 7 ESTs above.
    HaB1和Ha7.7是从上述与蜱已知基因有相似性的EST中筛选而来。
    RESULTS: 14 genes were cloned and the 10 known genes are responsible for intracellular process of cell differentiation and matrix synthesis and secretion.
    结果 :成功克隆到 14个人PDLF与GF细胞差异表达基因 ,其中 10个为已知基因。 已知基因的功能多与细胞分化和细胞外基质的合成、分泌有关。
    Among the known sequences, mus musculus ab1-interactor1,homo sapiens CGI-99 protein, tissue inhibitor of metalloproteinase 3 and homosapiens nuclear receptor co-repressor were up-regulated while homo-sapiens nuclear matrix protein p84 and coatomer protein complex, subunit gamma 2 were down-regulated.
    已知基因中上调的是musmusculusab1-interactor1,homosapiensCGI-99protein,tissueinhibitorofmetalloproteinase3,homosapiensnuclearreceptorco-repressor,下调的是homo-sapiensnuclearmatrixproteinp84和coatomerproteincomplex,subunitgamma2。
    Results: In the apoptosis group, 30 kinds of genes expressed in cervical intervertebral disc chondrocytes were screened out, in which 10 were up-regulated (ratio>2) and 20 down-regulated (ratio<0.5) as compared with the normal control group.
    结果:诱导凋亡组与正常组比较,共筛选出30条表达有差异的已 知基因,20条基因表达量明显下降(ratio<0.5),10条基因表达量明显上升(ratio>2);
    There were 11 putative genes and 20 known genes, including LGALS1, MT2A, COX1, RNP24, ATP1B3, IGL, IGH VDJ, RARRES2, GYPA, NEB and NPC2.CONCLUSIONS:Thirty-one proteins interacting with NGAL are obtained and an approach is offered for further exploring roles and biochemistry mechanisms of NGAL protein.
    已知基因中含有编码区的有LGALS1、 MT2A、COX1、RNP24、ATP1B3、IGL、IGH VDJ、RARRES2、GYPA、NEB和NPC2。 结论: 筛选得到31种可能与NGAL相互作用蛋白的 基因,这为研究NGAL功能及作用机制提供了 新途径。
    Methods Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cRNA was labeled with biotin(biotin-labeled cRNA as a probe). The cRNA probes were hybridized with Affymetrix GeneChip MOE430A (containing 22 690 transcripts,including(14 500) known mouse genes and 4 371 ESTs).
    方法分别提取Hca-F和Hca-P细胞的总RNA,反转录合成生物素标记的cRNA探针,并与A ffy-m etrix GeneCh ip MOE430A(包括22 690个转录本,对应于约14 500个小鼠已知基因和4 371个EST)杂交,检测结果利用生物信息学进行分析。
    Gene chip contained 9 984 human cDNA segment, which was prepared and provided by City University of Hongkong (UniGEMV2 cloneset known gene and ESTs were purchased from Incyte Company), Superscript Ⅱ reverse transcriptase was provided by Gibco-BRL Company. Fluorochrome Cy3 & Cy5 was the products of Amersham Pharmacia Company.
    基因芯片含9,984个人类cDNA片段,由香港城市大学准备和提供(UniGEMV2cloneset已知的基因和ESTs购自Incyte公司),SuperscriptⅡ反转录酶由Gibco-BRL公司提供,荧光染料Cy3&Cy5为AmershamPharmacia公司产品。
    NPHS1, NPHS2, ACTN4, TRPC6, CD2Ap and WT1 were not the disease-causing genes for family A;
    已知基因NPHS1、NPHS2、ACTN4、TRPC6、CD2AP和WT1不是家系A的致病基因;
    At present, the known CLCAs include mCLCA3 (mouse) and hCLCAl (human).
    这种功能独特的CLCA已知基因序列有:人hCLCA1、小鼠mCLCA3。
    Among the 122 known genes we further identified 20 up-regulated and 36 down-regulated genes.
    已知基因中有显著差异表达基因 5 6条 ,其中上调基因 2 0条 ,下调基因 36条。
    METHODS: Total RNA was isolated from Hca-F and Hca-P cells, and synthesized into double-stranded cDNA, then synthesized into biotin-labeled cRNA probes by in vitro transcription. The cRNA probes were separately hybridized with Affymetrix GeneChip? 誖 MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs), and the signals were scanned by the GeneArray Scanner.
    方法分别提取Hca-F和Hca-P细胞的总RNA,反转录合成双链cDNA,通过体外反转录合成生物素标记的cRNA探针,cRNA探针经片段化处理后分别与AffymetrixGeneChip誖MOE430A(包括22690个转录本对应于约14500个小鼠已知基因和4371个EST)杂交,杂交信号经扫描等处理并用生物信息学对检测结果进行分析。
    Results 210 acute rejection related genes were different between the groups (96 genes do wnregulated, 114 genes upregulated). 33 genes have been known (13 genes downregu lated, 20 genes upregulated) and 177 genes have not been known (83 genes downreg ulated, 94 genes upregulated), and 15 known genes (2 genes downregulated, 13 gen es upregulated) kept unreported.
    结果两组之间杂交信号有明显差异,同种异基因心脏移植术后差异表达基因共有210条(下调96条,上调114条),其中已知基因有33条(下调13条,上调20条),未知基因177条(下调83条,上调94条); 已知基因中有15条(上调2条,下调13条)尚未见报道。
    The known genes contained enzymes,cellular sig- nal molecules,gene regulators,transcriptional factors,oncogenes,genes related with anti-apoptosis factors, adhesion and growth regulators.
    已知基因中包含酶、细胞信号转导、基因调控因子、转录调节因子、癌基因、抗凋亡因子及细胞的黏附与生长调节因子等相关基因。
    There were 96 genes expressed differently in three cases and among them 77 genes were already known and the expression of 48 genes were up-regulated (ratio>1.0), and 29 down-regulated (ratio<0.5).
    已知基因中48条表达上调(ratio>1.0); 29条表达下调(ratio<0.5)。
 

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