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    SUSPENSION CULTURE OF STEM CALLUS CELLS OF PANAX GINSENG
    人参茎愈伤组织的细胞悬浮培养
    Effect of N~6-(Δ~6-isopentenyl) adenine and N~6-isopentyladenine on Cytokinin Oxidase in Phaseolus Callus Tissues
    i~6Ade和Hi~6Ade对芸扁豆和棉豆愈伤组织细胞分裂素氧化酶的影响
    STUDY ON CYTOKININ BINDING PROTEIN FROM CELL OF BAIBAILI TOBACCO CALLUS
    白百利烟草愈伤组织细胞细胞分裂素结合蛋白的研究
    CELL TYPE AND FUNCTION AND INTERNAL EMBRYOID IN WHEAT IMMATURE EMBRYO INDUCED CALLUS
    小麦幼胚愈伤组织的细胞类型、功能与内生胚状体
    Scanning Electron Microscopy of ODO Freeze Fracture on Embryogenic Callus Cells of Barley
    大麦胚性愈伤组织细胞ODO冷冻断裂面的扫描电镜观察
    Effect of 60 Co γ Radiation on Calli of Haynaldia Villosa
    60Coγ射线对簇毛麦愈伤组织细胞的分裂及染色体的影响
    Effect of NAA and 2,4 D and their Concentrations on Chromosomeploidy of Potato Calli in vitro Culture
    奈乙酸、2,4-D对马铃薯愈伤组织细胞染色体倍性的影响
    Identification of Gravity-related Esterases (grEST1 and grEST2) in Carrot Callus Cells
    胡萝卜愈伤组织细胞中与重力相关酯酶(grEST1和grEST2)的鉴别
    CELL SUSPENSION CULTURE OF HYPOCOTYL CALLI OF ISATICA INDICAGO
    菘蓝下胚轴愈伤组织细胞悬浮培养
    The Study of Human Interferon-alpha 2b Expressed by Ginseng Callus Cells
    人参愈伤组织细胞表达人干扰素α 2b的研究
    1. Analysis of the components in callus and cell suspension cultures of Ligusticum chuanxiong Hort.
    1.川芎愈伤组织、细胞悬浮培养体系的建立及其化学成分气相色谱—质谱联用分析:诱导并建立了川芎愈伤组织、细胞悬浮培养体系。
    1. The cell DNA content of thirty-five citrus calli of different genotypes were measured for 3 times by flow cytometry during 4 years.
    1、本试验在4年中连续3次使用流式细胞仪,对继代培养多年的柑橘愈伤组织的细胞DNA含量进行测定。 结果发现,待测的35种愈伤组织均存在细胞DNA含量加倍的现象。
    Correlation analyses suggested that the effect of DNA content variation on competence for somatic embryogenesis of citrus calli was not significant and the coefficient of correlation was minus 0.1008 (P<0.01) ;
    相关性分析表明,柑橘愈伤组织细胞DNA含量变异百分率与体细胞胚胎发生能力之间的相关性较小,相关系数为r=-0.1008(P<0.01),未达到显著水平。
    The possible reason was that the cell structure and function of leaves was different from the other organs', and the amylase cell induced from roots, stems, petioles appeared dissimilar physiological and biochemical function to the leaves', consequently as embodied that amylase' activity showed different changing trend under the same concentration PEG stress.
    这一结果显示由根、茎、叶柄诱导的愈伤组织细胞的生理生化功能比较相似,而和叶片诱导的愈伤组织细胞存在差异。 这可能是由于叶片的细胞与其它三种器官的细胞结构和功能差异较大,而导致叶片诱导出的愈伤组织细胞与根、茎、叶柄诱导的愈伤组织细胞表现出不同的生理生化功能,从而体现在对相同的PEG胁迫条件下,淀粉酶活性显示不同的变化趋势。
    2. Different concentration of L-Phe in the MS culture medium has different effects on the cell activity of tobacco callus.
    2.MS培养基中添加不同浓度的L-苯丙氨酸对愈伤组织的细胞活性有较明显的抑制作用,当浓度为1~2 mmol·L~(-1)时,烟草愈伤组织细胞活性下降较少;
    1-2 mmol·L~(-1) L-Phe's has slightly inhibit effect, while the concentration is 4 mmol·L~(-1), the inhibit effect are severely, the cell activity decline to a very low level.
    当浓度为4mmol·L~(-1)时,烟草愈伤组织生长受到严重抑制,活性下降至非常低的水平,仅当浓度为3mmol·L~(-1)时,愈伤组织细胞活性下降50%左右,较符合筛选体系对筛选剂的要求。
    The cutting pieces of bulb of Fritillariae pallidiflorae were cultured on MS media supplemented with 2, 4-D, IAA, NAA or 2, 4-D+KT、 IaA+KT, NAA+KT. (2, 4-D, IAA, NAA 1mg/l, KT 0.1mg/l). Chromsome variations in calli and callus differentiation and chromosome ploidy of regenerated plants were studed.
    伊贝母鳞茎培养在附加2,4—D、IAA、NAA和2,4—D+KT、IAA+KT、NAA+KT的MS培养基上(2,4—D、IAA、NAA1毫克/升,KT0.1毫克/升),研究了愈伤组织细胞染色体的变异及愈伤组织的分化和再生植株的染色体倍性。
    In vitro screening of seed-derived and microspore-derived callus lines was carried out by the biotechnology of plant cell culture, with the raw toxin extract of piricularia oryzae as stress factor.
    利用植物细胞培养离体筛选生物技术,以稻瘟病菌粗毒素提取液作为外源胁迫因素,对水稻种子体细胞和花粉性细胞无性系,进行愈伤组织细胞培养抗性筛选或花药培养抗性筛选。
    The neomycin phosphotransferaseⅡ(NPTⅡ)gene of the plasmid pLGVneo2103 was transferred into the tissue cells of tobacco leaves and the calli of corn and soybean by using the condenser discharge mode electroporation system, which was designed and constructed by our laboratory.
    用自制的电容放电式电激系统,将质粒pLGVneo2103上的NPTⅡ基因直接导入到烟草叶片组织细胞和玉米、大豆的愈伤组织细胞中。
    Cytokinin binding proteins (CB-protein) which moleculer weight arc about 4400±100 dalton from cell of Baibaili tobacco callus was purified by affinity chromatography on benzylaminopurin-Knked sephrosc 4B column.
    利用BA-Sepharose 4B亲和层析技术丛白百利烟草(Nicotiana tabacum Baibaili)愈伤组织细胞分离提纯了分子量为4400±100道尔顿的细胞分裂素结合蛋白(CB-蛋白)。
 

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