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    Analysis on Result of Double Crossing Some Characters of Tungoil Tree
    油桐若干性状双列杂交结果的分析
    Effects of Exhaustive Swimming on Morphology of Islet A and B cells in Mice
    力竭性游泳对大鼠胰岛 A、B 细胞形态学变化的影响——免疫组化及原位分子杂交结果分析
    At the same time,ethylene production measurement and storage experiment of tomato fruits were carried out. 【Result】The hybridization results indicated that the expression of fruit ripening-related genes,such as LeACO3 and LeEBF1,and pathogenesis-related protein genes,such as LePR1,LePR5 and LeNP24,were reduced sharply.
    结果Northern杂交结果表明,番茄ACO1基因表达被抑制后,与果实成熟相关基因LeACO3和LeEBF1,以及病程相关蛋白基因LePR1、LePR5和LeNP24的表达量急剧降低。
    Methods The serum samples from 48 patients infected with HBV were detected by gene chip technique for HBV genotypes、YMDD wildtype and YVDD or YIDD mutations,some positive samples had been testified by HBV preS/S gene sequencing.
    方法抽提HBV DNA,用PCR方法扩增S基因及P基因部分区域,扩增产物与基因芯片上的寡核苷酸探针进行杂交,杂交结果通过芯片影像读取仪扫描到计算机上,经软件分析可得到HBV基因型、HBVYMDD野生型及YVDD、YIDD变异型结果; 部分标本通过HBV preS/S基因序列测序证实。
    The RNA isolated by the improved method showed clear bands of 28S rRNA and 18S rRNA,and the value of OD260/OD280 is 1.8 to 2.1.RNA isolated by the improved method could ba used for RT-PCR and Northern blot.
    提取的RNA质量较高,纯度检测表明RNA样品OD260/OD280在1.8-2.1之间,含量约为10μg/μL。 RT-PCR和Northern杂交结果表明RNA可以满足进一步分子操作的需要。
    The results of in situ antisense RNA hybridization showed that the expressions of BMP2b and HAS2 in the zebrafish heart were reduced in the MTX treatment group at 36 and 48 hpf.
    胚胎整体原位杂交结果显示MTX处理组斑马鱼心脏发育相关基因BMP2b及HAS2在心脏的表达于36 hpf及48 hpf下调。
    The in situ hybridization result and the immunohistochemical result is basic one.
    原位杂交结果与免疫组化结果基本一致。
    The Northern blot hybridization results indicated that the PR-NP24 expressed mainly in fruit and root,and it was inhibited in the wounded leaves of the wild type tomato and the Nr tomato,but its expression in the wounded leaves of the rin tomato had no significant change.
    杂交结果表明,该基因主要在果实和根部表达,伤害抑制WT、Nr番茄叶片中该基因的表达,而对rin番茄叶片无明显影响;
    The results of 3 hybridization solutions were as follows: 0.15% SDS better than 0.2% SDS,10×SSC better than 5×SSC.
    3种杂交液的杂交结果比较:0.15%SDS效果好于0.2%SDS,10×SSC效果好于5×SSC;
    Using Th. intermedium genomic DNA as a probe, Southern-blotting analysis indicated that these DNA fragments originated from the target chromosome.
    以中间偃麦草基因组DNA为探针的Southem杂交结果表明,这些染色体DNA片段来自目标染色体。
    RESULTS: The results of hybridization showed that the specific probes of Pe Pg Pi Pn on microarray reacted only with corresponding PCR products of Pe Pg Pi Pn, not react with others. Pe Pg Pi Pn could be identificated accurately and rapidly from root canal microbial samples by this microarray.
    结果:杂交结果显示芯片上的Pe、Pg、Pi和Pn4种探针只能和相对应的Pe、Pg、Pi和Pn的PCR产物反应,而与其他标准菌株的PCR产物无反应,并且用此芯片能从临床感染根管细菌学标本中准确快速的检测出Pe、Pg、Pi和Pn。
    DNA fixation technique on influence of hybridization results of PCR products of M.tuberculosis
    DNA固定技术对结核分枝杆菌PCR产物杂交结果影响的研究
    Background of gene chip reduced with purified probes
    纯化探针降低基因芯片杂交结果的背景
    Influence of Sample Preparation and Amplification Conditions on Oligonuleotide Chip
    样品制备与扩增条件对寡核苷酸芯片杂交结果的影响
    Two PCR Amplification Methods Influence the Membrane Chip Hybridizations Results for Detection of Group A Rotavirus
    两种PCR方法对检测A组轮状病毒膜芯片杂交结果的影响
    3 genomic DNA samples from PCR positive plants were selected for Southern blot analysis, the result showed all the sample had hybridization signal using CMV Pl-RP gene probe, and no hybridization signal was observed in control.
    取PCR鉴定为阳性的3个植株,进行Southernblot印迹分析,其杂交结果均为阳性,该结果表明CMV P1-RP基因已整合于烟草基因组DNA中。
    Then the recombinant baculovirus BacPAK-PF4 wasidentified by plaque assay DNA dot blotting and southern hybridization showed thatthe baculovirus contains the hPF4 gene.
    DNA点杂交和Southern杂交结果均证实了PF4基因已经插入到了杆状病毒基因组中。
    Moreover, single copy of ICL1 gene in M. grisea was showed by Southern blot.
    基因组Southern杂交的结果显示M.grisea ICL1基因是单拷贝基因。
    (3) Northern blot showed that PBP-Harm and GOBP2-Harm are specifically expressed in the antenna of H.armigera.
    (3)Northern杂交结果表明:PBP-Harm和GOBP2-Harm都在棉铃虫触角中特异性表达。
    Accession number HSU20428 was given by NCBIgenebank in 1995, and was named by Gene Nomenclature Committee as ST14 (suppression of tumorigenicity 14) in 1998. The data from human genome project (HOP) shows that SNC19/ST14 gene contains 19 exons and 18 introns that span 50420 bp.
    在人类基因组计划的基因组资料11号染色体的基因图谱中已将SNC19/ST14基因收录。 SNC19基因的基因组DNA全长为50420bp,共有19个外显子及18个内含子,荧光原位杂交结果显示,该基因定位于11q24-25区。
 

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