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    Methods:Immunohistochemical method was used to detect the expression of P16INK4A in 30 cases of normal cervical tissues, 23 cases of CINⅠ,26 cases of CINⅡ, 31 cases of CINⅢ, and 22 cases of invasive squamous cell carcinomas of cervix(ISCC).
    方法:应用免疫组织化学方法检测30例正常宫颈组织、23例宫颈上皮内瘤变Ⅰ级(CINⅠ)、26例CINⅡ、31例CINⅢ和22例浸润癌组织中P16INK4A蛋白的表达;
    Results: The positive rates of nm23, TIMP-2 in breast invasive ductal carcinoma tissues, were higher than those in the fibroadenoma and ductal carcinoma in situ tissue;
    结果:乳腺浸润癌组织中nm23和TIMP-2蛋白阳性率低于乳腺纤维腺瘤和导管内原位癌组织(P<0.05);
    METHODS: The expression of P16INK4A and PTEN in 30 specimens of normal cervical tissues, 11 specimens of cancer in situ (CIS), and 24 specimens of invasive cervical carcinoma (ICC) was detected by SP immunohistochemistry;
    方法:应用免疫组织化学方法检测P16INK4A蛋白和PTEN蛋白在30例正常宫颈组织、11例原位癌、24例宫颈浸润癌组织中的表达。
    Results The expression of HPV and FHIT protein have obvious variability in normal skin squamous epithelial tissue,atypical hyperplasia tissue,carcinoma in situ and infiltrating carcinoma tissue(P <0.05).
    结果HPV和FHIT蛋白在癌旁正常皮肤鳞状上皮组织、非典型增生组织、原位癌及浸润癌组织中的表达具有明显差异性(P<0.05);
    Expression of CD44V6、PCNA、CⅣ in Cervical Invasion Cancer and Metastasis Potentiality
    宫颈浸润癌组织中CD44V6、PCNA、CⅣ表达与转移潜能的关系研究
    P16 protein overexpressioned in CIS and CC, there was low or negative expression of P16 in normal cervical tissue (4)There was strong relationship between p16mRNA expression and HPV16/18E7mRNA expression (r=0. 81322) (P<0. 05) ;
    HPV16/18E7阳性组p16mRNA的表达明显高于阴性组,其差别有统计学意义(P<0.05); P16蛋白在宫颈原位癌和浸润癌组织中呈现过表达,在正常组织中为阴性表达或弱表达;
    Methods Immunohistochemical staining, S-P method, was used to detect the positivity of PTEN in 23 cases of normal breast tissues, 28 cases of proliferative breast disease including 8 cases of atypical hyperplasia (ADH), 8 cases of ductal carcinoma in situ (DCIS) and 98 cases of breast cancer.
    方法:用免疫组织化学S-P法检测23例癌旁正常乳腺组织、28例乳腺增生组织(其中20例普通型增生,8例非典型增生)、8例导管原位癌、98例浸润性乳腺癌组织PTEN蛋白的表达,检测98例浸润癌组织中integrinβ1、MMP-2、MMP-9、TIMP-1、cyclinβ1蛋白的表达,并进行微血管密度计数。
    Atypical hyperplasia and DCIS expressed higher levels of PTEN thannormal tissues(P<0.05), but had no remarkable distinction with invasive breast cancer.
    浸润癌组织中PTEN的失表达率为7.1%,高表达率为49.0%,与正常乳腺组织比较有显著性差异(P<0.05),与增生的乳腺组织和导管原位癌组间无显著差异。
    Methods: The expression and distribution of MMP-7 and TIMP-1 proteins are detected by immunohistochemical technique in 53 cases of cervical carcinoma tissue, 9 cases of CIN and 10 cases of normal cervices tissue coming from operation specimen excised because of myoma of uterus.
    研究方法:本研究采用免疫组化SP法对53例宫颈浸润癌组织,9例CIN,10例因子宫肌瘤而行子宫切除的正常宫颈组织标本进行MMP-7及TIMP-1蛋白表达的检测。
    Objective To study expression of CD44V6、PCNA、CⅣ in the cervical invasion cancer and their metastasis potentiality.
    目的 探讨CD44V 6、PCNA、CⅣ在宫颈浸润癌组织中的表达与转移潜能的关系。
    Methods Expression of CD44V6、PCNA、CⅣ in 104 case of cervical cancer were detected by immunohistochemistry S P method.
    方法 采用免疫组织化学S P法检测 10 4例宫颈浸润癌组织中的CD44V 6、PCNA、CⅣ单克隆抗体表达情况。
    Results: 53 exons 5~8 and ADRB 2 were amplified successfully from 6 of 7 cases hyperplasia, 1 case squamous metaplasia, 10 of 12 cases dysplasia and 19 cases infiltration carcinoma microdissected.
    结果 :7例支气管粘膜上皮增生 ,1例鳞状化生 ,12例不典型增生 ,19例浸润癌组织都分别成功地显微切割并提取DNA。
    The expression of COX-2 was correlated with clinical stage,invasion and lymph node metastases (P< 0.05 ,P< 0.05 ,P< 0.001 ),but there was no correlation with age and tumor size.
    乳腺浸润癌组织COX 2的表达与临床分期、周围组织浸润和淋巴结转移密切相关 ,(P <0 .0 5 ,P <0 .0 5 ,P <0 .0 0 1) ,与患者年龄和肿瘤大小无明显相关性。
    The expression of P33/ING1 protein in esophageal invasive carcinoma was negatively correlated with that of Survivin protein( r =-0.480, P <0.01).
    食管浸润癌组织中P33/ING1蛋白的低表达与Survivin蛋白的高表达呈负相关 (r =- 0 .4 80 ,P <0 .0 1 )。
    Methods: Using streptavidin-biotin peroxidase(SP) method,the expression of Survivin,Bcl-2, and P53 protein in tissue of 10 cases of normal controls, 18 cases of atypical hyperplasia,21 cases of carcinoma in situ,and 69 cases of cervical invasive carcinoma were examined.
    方法:采用SP免疫组织化学方法, 检测10例正常宫颈组织,18例宫颈不典型增生组织,21例宫颈原位癌组织,69例宫颈浸润癌组织中Survivin、bcl -2 及P53蛋白的表达。
    Results P 33ING1 positive expression in normal cervical tissue is 100%(10/10); the expression in carcinoma-in-stiu and cervical invasive carcinoma tissues is obviously lower,57.1% (11/21) and 59.4%(41/69) than that in normal cervical tissues. The experssion of P 33ING1 was related to that of P_ 53 in cervical carcinoma.
    结果 对照组中P33ING1均阳性表达100%(10/10)而宫颈原位癌和宫颈浸润癌组织中表达分别为57.1%(11/21)和59.4%(41/69),明显下降,与对照组之间差异显著P33ING1和P53的表达有相关性。
    Infiltrating densities of CD45RO+T lymphocytes were significantly higher in cases of DCIS,MIC and IDC than in other intraductal hyperplastic lesions(P<0.05).
    导管原位癌、微浸润癌及浸润癌组织中CD45RO+T细胞浸润密度均高于其它病变组织(P<0.05)。
    (2) There was no significant difference between the carcinoma in situ and invasive carcinoma (P>0.05), but in poor differentiation cervical carcinoma, p185 protein was highly expressed (56.8%). The expressions of p185 proteins was related to clinical stage (P<0.05).
    (2)p185蛋白在原位癌组织和浸润癌组织中的表达差异无统计学意义(P>0.05),但低分化鳞癌中p185蛋白表达与原位癌比较,差异具有统计学意义(P<0.01),II期和III期宫颈癌患者组织中的p185蛋白表达明显高于0期和I期的患者(P<0.05);
    Methods:We detected the expression of cyclin G1 in normal cervical epithelium/cervicitis(control group,20 cases),CIN(CIN1 26 cases,CIN2 26 cases,CIN3 28 cases) and invasive cervical squamous-cell carcinoma(SCC,20 cases)with immunohistochemical method.
    方法:用免疫组化二步法检测cyclin G1在正常/宫颈炎组织(20例次)、宫颈上皮内瘤变(CIN)1~3级(80例次)和浸润癌组织(20例次)中的表达。
    RESULTS:1)The positive rates of Sle x in rectal carcinoma, normal rectal mucosa, distal intramural spread, metastatic lymph nodes, isolated mesenteric cancer node and metastatic liver focus of rectal carcinoma were 80.00%, 0, 100.00%, 100.00%, 100.00% and 100.00%, respectively.
    结果:1)Slex 在直肠癌组织、正常肠壁组织、远端肠壁浸润癌组织、阳性癌转移淋巴结、癌结节及肝转移灶中的表达率分别为80. 00%、0、100. 00%、100. 00%、100 .00%和100 .00%。
 

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