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    Identification and Corresponding Genes Chromosome Location of del1 Mutant That Was Deficient in Resistance to Herbivores in Tomato
    番茄(Lycopersion esculutum)抗虫缺失突变体def1的鉴定及相应基因的定位
    Some of the clones were sequenced and five new genes were found which were named UL33、UL34、UL45、UL46 and UL47.They showed homology to the corresponding genes of the members of Alphaherpesvirinae by sequence alignments on a certain extent.
    选取部分克隆进行序列测定,获得5个UL片段基因,分别为UL33、UL34、UL45、UL46和UL47。 序列比较显示,它们与α-亚科疱疹病毒其他成员相应基因具有不同程度的同源性。
    Methods To amplify the class1,2 and 3 integrase genes in 184 gram negative bacilli from medistream urine by polymerase chain reaction with universal primer of 3 genes.
    方法用1、2、3类三种整合酶基因通用引物扩增184株中段尿中分离革兰阴性杆菌的相应基因;
    Results Specific HBV gene fragment was obtained with self-designed primer.
    结果:扩增产物的核酸序列分析表明,设计的引物序列在确定的条件下可以特异性的扩增HBV相应的基因片段。
    The further sequence analysis showed that 12S rRNA and 16S rRNA gene of Sichuan black bear had a higher evolution rate,which had more differences than that of American black bear,brown bear,polar bear,spectacled bear and panda,but Sichuan black bear had more homology with American black bear.
    通过进一步的序列分析表明,四川黑熊的12S rRNA和16S rRNA基因有较高的进化速率,与美洲黑熊、棕熊、北极熊、眼镜熊及大熊猫的相应基因相比较有较大差异,其中与美洲黑熊的同源性相对较高.
    The gene were sequenced and compared with those of other IBV strains from China in GenBank. The results showed that the nucleocapsid genes of strain W and C9001 shared 88.0 % nucleotide identity and 89.5 % amino acid homology.
    结果表明,扩增的2个IBV分离株核衣壳基因片段长度均为1230bp,编码409个氨基酸,彼此间核苷酸和氨基酸同源性分别为88.0%和89.5%,与GenBank中有代表性的参考毒株相应基因核苷酸和氨基酸序列比较显示,W株核苷酸序列与GenBank中的广东分离株(AY646283)同源性最高,为94.1%,氨基酸序列同源性为94.6%;
    Influence of insulin on neuronal apoptosis after cerebral ischemia reperfusion in the Wistar rats and regulation of related genes
    胰岛素对大鼠脑缺血再灌注时神经元凋亡及其相应基因的调控
    Three Bombyx mori Genes,chi,gluE and fruA,Encode Proteins Homologous to Microorganism and Primary Analysis of Horizontal Gene Transfer
    家蚕chi、gluE和fruA基因与微生物相应基因的同源性及基因水平转移初探
    Variety of differential display on gene and corresponding gene′s clone following corneal alkali burns in rats
    大鼠角膜碱烧伤后差异表达基因的变化及相应基因克隆
    1. Blasting in the genebank with the 300bp v62 gene difference displayed between MDR cell line SGC7901/VCR and its parental cell line, we found that there were several genes showing high homology with it.
    1、以消减杂交所得到的在胃癌耐药细胞系SGC7901/VCR与其亲本细胞中差异表达的400bp左右的V62基因片段序列检索基因库,发现该基因片段与多种基因高度同源,通过相应的基因序列可以分为4类。
    hspA sequence of MEL-HP27 had high homology from 95.76% to 97.46% as compared to the nucleotide reported in Genbank and putative amino acids homology was from 95.76% to 97.46%.
    MEL一HP27 ureB基因序列与GenBank公布的Hp相应基因序列同源性高达%. 08%一98.30%,氨基酸序列同源性98.77%一99.82%。
    It was found that one EST was preferentially expressed in the intact ovules of Xu142. Its corresponding gene, GhABC1, encodes an ATP-binding cassette (ABC) transporter protein.
    该EST相应的基因GhABC1编码一个ABC转运蛋白(ATP-binding cassette transporter)。
    CYP11B2 is a gene to be tested for association with EH, especially salt sensitive hypertension.
    醛固酮合成酶CYP11B_2是醛固酮合成的关键酶,相应基因位于8号染色体上。
    In this study, T-DNA insertional mutagenesis and RNA interference (RNAi) strategies were used to create rice mutants, and functional analysis on corresponding genes and their expression patterns were carried out based on their mutation phenotypes.
    本研究利用T-DNA插入突变和RNA干涉技术创制水稻突变体,并根据突变体的表型来研究相应基因的功能和表达模式。
    . The special primers for PPARγ, C/EBPα, ADDl, LPL, AP2, adipsin and P-actin were designed respectively according to the reported gene sequences. Then genome RNA was extracted from the subcutaneous adipose tissue of pigs and the target gene mRNA amplified through RT-PCR with special primers.
    根据Genebank登陆的PPARγ,C/EBPα,ADD1,LPL,AP2,adipsin和内标β-actin基因序列设计引物,分别以皮下脂肪组织RNA为模板,经反转录和PCR,克隆得到了相应的基因片断并进行测序。
    Based on the gene clone, the suitable anneal tempreture, MgCl_2 concentration, and cycles in PCR system were discussed and the optimal semi-quantitative RT-PCR methods were constructed to detect the amounts of all genes mRNA expression in pigs.
    在此基础上,进一步探讨了PCR体系中适宜的退火温度、MgCl_2浓度和循环次数,构建了适合于相应基因序列的优化半定量RT-PCR法,用于不同生长阶段猪皮下脂肪组织PPARγ,C/EBPα,ADD1,LPL,AP2和adipsin基因mRNA水平的测定。
    Southern blottingreveals that this gene is wheat genomic gene.
    Southern印迹分析结果表明TaMRE-BP cDNA片段的相应基因存在于小麦基因组中。
    [2] At protein level, the concentrtion-effect curve and optimal concentrations of SP's effects on fibroblasts intrinsic growth factors protein synthesizes were similar to and its peak time points (16h-36h) were later to that at gene level.
    ②在蛋白质水平,SP对成纤维细胞EGF/EGFR、bFGF/FGFR-1、TGFβ-1/R-1/R-2合成的量-效曲线和最佳浓度基本与基因水平的表现相似,但其高峰作用时间(16h-36h)迟后于相应基因表达的高峰时间。
    Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding PCR product from Brussel Sprouts, Kohlrabi and Kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon.
    对各PCR产物分别进行回收、纯化、载体连接和序列测定及基因结构分析等,结果表明,该片段在抱子甘蓝、羽衣甘蓝和球茎甘蓝三种作物中的全长分别为1665bp、1650bp和1650bp,包括相应基因的前两个外显子和内含子以及第三个外显子的22bp序列。
    Since some Shigella flexneri 2a genes, including yphF wcaJ dam alkA cysG yaiC IS600 citC aroD CPO198, possibly related to virulence were isolated by using in vivo expression technology (IVET), these genes mutants were constructed and the relationship between these genes and the virulence of this pathogen was researched.
    本文用体内表达技术获得了痢疾杆菌福氏2a可能与其毒力相关的基因,包括yphF、wcaJ、dam、alkA、cysG、yaiC、IS600,citC、aroD、CPO198等,通过构建相应基因的突变体,研究二者之间的关系。
 

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