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    The equation, which was made from computer in regressive analysis, M% = 2.54+8.69 (Pp/Ps) + 0.23(Rp) - 0.04(DPA) (F = 0.2, P< 0.01). was suggested by the authors with a primary standard of national thickness of the medial muscular coat of the "transitional pulmonary arterioles".
    作者推荐经电脑分析的以心导管指标反映定量活检的方程:M%=2.54+8.69(Pp/Ps)+0.23(Rp)-0.04(DPA),以及国人“移行段”肺小动脉中层厚度的分析标准,供临床参考。
    The severity of HD symptoms was significantly correlated with 8 items including the length of the narrow and transitional segment, enterocolitis, and etc. but not with the other 6 factors including the narrow segment and density of nerve fibers in the narrow segment.
    对202例HD患儿的症状与14项临床和病理因素进行逐步回归和Logistic分析,发现HD的症状严重程度与狭窄段移行段总长、肠道炎症等8项因素有关,而与狭窄段长度、狭窄段中神经纤维增生程度等6项因素无关。
    Methods Expression of RET protein in the in testinal tissue from 42 cases of sporadic HD patients, (HD group), and 10 cases of colon tissue without HD (control group), were detected by using immunohistochemical technigue with three RET protein antibodies and antiphosphotyrosine antibody, respectively.
    方法 利用抗RET蛋白的三种抗体,以及抗磷酸酪氨酸抗体采用免疫组织化学方法,对42例散发性HD肠组织中RET蛋白表达情况进行研究,10例正常结肠作对照。 结果 抗RET蛋白的三种抗体在HD扩张段、移行段、对照组均显强阳性染色,P>O.
    It had negative relationship with the qualitative pathology analysis(r=-0.697,P<0.05)and vWF expression in the peripheral venous plasma (=86.511-0.26x, r=-0.649, P<0.01).
    术前外周静脉血浆vWF水平与“移行段”小动脉内膜vWF表达呈负相关 (^y =86 . 5 11- 0 . 2 6x ,r =- 0 . 6 4 9,P <0 . 0 1)。
    Methods The mRNA of NGF and NGFR in normal, spastic, transitional and dilated segment were detected by RT-PCR in 22 fresh samples of HD.
    方法 采用RT PCR技术检测新鲜标本正常对照肠管、痉挛段、移行段和扩张段各 22例NGF和NGFRmRNA的表达,分析并统计其与临床病理特点之间的关系。
    Conclusions NGF and NGFR genes may play some roles in the genesis of HD.
    HD的痉挛段和移行段肠管NGF及NGFR表达异常,提示NGF和NGFR基因可能与HD的发生有关。
    The expression rate for NT-3 and Trk C in ganglion cells of HD transitional segments (53.1%, 28.1% respectively) was significantly lower than that of dilation segments (93.8%, 43.8% respectively, P < 0.05).
    移行段神经节细胞NT 3、TrkC阳性率(5 3 .1%、2 8.1% )均较扩张段(93 .8%、43 .8% )显著降低(P <0 .0 5 )。
    Hud protein level in strictural and transitional segment remarkable low than that in dilated and normal segments(P<0.05) in Hirschsprug's disease.
    狭窄段和移行段的HuD蛋白水平明显少于扩张段和正常段(P<0.01)。
    Methods Immunohistochemical staining for laminin was done in the aganglionic, transitional and normal segment in 27 cases with HD. The staining area was calculated by JD 801 pattern analysis system.
    方法用二步法对27例HD患儿狭窄段、移行段、正常段肠壁组织进行免疫组织化学染色,RSImage成像系统照相,JD801形态学图像分析软件判定阳性染色面积;
    Quantitative vWF expression in the endothelium of pulmonary transitional arterioles in pulmonary hypertension secondary to congenital heart defect
    先心病合并肺高压者“移行段”肺小动脉内膜的vWF定量表达分析
    The SlOO is expressed in the nervous fibers of all the four segments of HD colon.
    HD肠管正常段、扩张段、移行段和狭窄段等各段肌间及粘膜下神经纤维5100均表达,呈棕黄或棕褐色;
    The research is toinvestigating the role of ICC (the interstitial cell of Cajal) on thepathogenesis of Hirschsprung's disease by observing thedistribution of ICC in HD.
    本研究通过观察Cajal间质细胞(interstitial cell of Cajal,lCC)在先天性巨结肠患者狭窄段、移行段、扩张段中的分布情况,探讨lCC在先天性巨结肠发病中的作用。
    Results : The density of the interstitial cells from the dilatedsegment to the transitional segment and aganglionicsegment decreased gradually.
    结果:发现ICC的密度从扩张段一移行段一狭窄段是逐渐减低的,在狭窄段少见。
    There is no significant difference between normoganglionic zone and the control group. The content in transitional zone is higher than that in normoganglionic zone (485.89+50.55ng/460.36?9.89ng) (P<0.05).
    经两两比较,狭窄段LN的含量高于其它各组(尸<0.05),移行段LN的含量高于扩张段与对照组(尸<0.05),扩张段LN的含量与对照组相比,差异没有显著性(外0.05)。
    The aganglionic zone has higher content than the transitional zone (513.64?3.00ng/485.89 ?0.55ng)(P<0.05). 2. The content of collagen IV in normoganglionic zone is 121.86 ?
    (2)W型胶原在HD狭窄段每毫克平滑肌组织的含量为177.86士21.76飞,在移行段为134.25士20.86雌,在扩张段为121.86士20.39雌,在正常对照组为1巧.
    The current study is to investigate the role of ICC and ganglionic cells in the pathogenesis of HD through observing the distribution of the two types of cells in normal colon and HD's different segments.
    本研究通过观察神经节细胞与Cajal细胞在正常结肠及先天性巨结肠患者痉挛段、移行段、扩张段的分布,探讨先天性巨结肠的发病机制。
    Methods The aganglionic ,transitional and dilated segments were collected in operation from 25 children with Hirschsprung's disease in Shandong Provincial Hospital. Another 6 normal segmengts were also collected.
    方法 收集山东省立医院25例先天性巨结肠患儿标本,于手术中分别选取扩张段、移行段、痉挛段肠壁的全层组织,另取6例正常结肠全层组织标本,常规固定石蜡包埋组织切片备用。
    The number of ganglion cells and intramuscular nerve fibers became normal at 8cm from transitional segments in this study(P>0.05).
    4、肌内神经纤维,Syn、NCAM表达在狭窄段平滑肌层明显增强,环肌层的表达强度高于纵肌层,随着远离移行段肌层内神经纤维的阳性表达逐渐减低,在8cm与对照组无明显差异(P>0.05),有3例肌内出现增粗
    Methods The expression of RET proto oncogene in the aganglionic, transitional and dilated segments of colon from 12 children with Hirschsprung's disease was studied by RT PCR method. G3PDH was adopted as control.
    方法采用RTPCR技术对12例先天性巨结肠的狭窄段、移行段及扩张段分别做RET基因的表达,并以G3PDH作内参标,观察RET基因的表达情况。
    Results The expression of RET proto oncogene from the dilated segment to the transitional segment and aganglionic segment decreased gradually.
    结果发现RET基因的表达从扩张段→移行段→狭窄段是逐渐减低的。
 

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