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    EXPERIMENTAL STUDIES ON THE IN VITRO SENSITIVITY OF ADRIAMYCIN (ADM),ACLACINOMYCIN A,B,(ACM A,B)FOR Tca8113 CELL LINE
    用体外微量法测定亚德里亚霉素、阿克拉霉素A、B对人体舌鳞状细胞系的药敏试验研究
    REGULATION OF GLUCOCORTICOID RECEPTOR BY GLUCOCORTICOIDSs STUDY ON THE HUMAN HEPATOCELLULAR CARCINOMA CELL LINE (SMMC-7721)
    糖皮质激素对糖皮质激素受体的调节 Ⅰ.人体肝癌细胞系(SMMC-7721)的研究
    The effects of swine bile acids-sodium salt on the inhibition of proliferation and induction of differentiation in human promyelocytic leukemia cell line HL-60
    猪胆汁酸钠对人早幼粒白血病细胞系HL-60的增殖抑制及分化诱导作用(英文)
    INHIBITORY EFFECT ?N HUMAN PROMYELOCYTIC LEUKEMIC CELL LINE HL-60 OF PORIATIN
    茯苓素对人白血病细胞系HL-60增殖的影响
    The Influnce of "Vitality Reinforcing Prescription" on PTH Receptor Binding in ROS 17/2.8 Cell Line
    中药“固真方”对ROS17/2.8细胞株PTH受体结合率的影响
    Effect of Tunicamycin on the Insulin Receptor on the Surface of Human Hepatocarcinoma Cell Line SMMC-7721
    衣霉素对人肝癌细胞表面胰岛素受体的影响
    Effect of Prednisolon, Vincristine and Mitoxantrone on Glucocorticoid Receptor in HL-60 Cell Line
    强的松龙、长春新碱及米托蒽醌对HL-60细胞株糖皮质激素受体的影响
    Study of metal compounds on unscheduled DNA synthesis in human amnion cell line FL
    金属化合物诱发人羊膜FL细胞非程序性DNA合成的研究
    Forskolin augments the effect of glucocorticoids on proliferation and differentiation of a human osteosarcoma cell line by up-regulation of glucocorticoid receptor
    Forskolin加强糖皮质激素对人骨肉瘤细胞系增殖分化的调节作用
    Studies on the growth inhiibitory effect of Esculin on human pulmonary adenocarcinoma cell line A-549 and its mechanism of anti-inflammation and anti-asthma
    七叶苷对人肺腺癌细胞株A_(549)的生长抑制作用及其抗炎平喘机制的探讨
    Expression of Human IL-2 cDNA in the Human Osteosarcoma Cell Line
    人白细胞介素-2基因在人骨肉瘤细胞系内表达
    Oxytocin stimulated insulin release in a clonal β cell line
    缩宫素刺激克隆的β───细胞系RINm5F内胰岛素释放:涉及磷脂酶C依赖性及非依赖性通道
    Establishment of a human bladder cancer cell line with multidrug resistance──BIU-87/ ADM
    人膀胱癌BIU-87细胞系多重抗药性的形成
    STUDY ON THE INDUCTION OF PLACENTAL ALKALINE PHOSPHATASE IN ECA 109 CELL LINE BY PREDNISOLONE
    强的松龙对Eca109细胞胎盘型碱性磷酸酶的诱导作用机制研究
    CYTOTOXIC EFFECT OF KA JIE KE IE ON TUMOR CELL LINE K_(562) IN VITRO
    卡介克蟹对肿瘤细胞株K_(562)的体外杀伤效应
    ESTABLISHMENT OF HUMAN BREAST CANCER MULTIDRUG RESISTANT CELL LINE AND ITS CHARACTERIZATION
    人乳腺癌多药耐药细胞株的建立及其特性的研究
    Methods: Cell line CHO-K1 was transfected with pcDNA3.1(+)-hGPCRc and then selected with G418 at 800 μg/ml for 10-14 days. After transfection and selection,the cell line CHO-hGPCRc was identified by RT-PCR for hGPCRc mRNA stable expression.
    方法:将重组表达质粒pcDNA3.1(+)-hGPCRc转染入CHO-K1细胞,经G418(800μg/m l)作用7~10 d,挑选、扩增阳性克隆,得到CHO-hGPCR c细胞,再以RT-PCR检测所得细胞内hGPCRc mRNA的表达;
    Methods After transfection of pcDNA3.1 Human AP OBEC3G-Myc-6Xhis into HepG2.2.15 cell line,the expression of APOBEC3G were detected at mRNA and protein levels by RT-PCR and Western Bolt,respectively. ELISA and RT-PCR were applied to measure the alteration of expressions of HBsAg and HBeAg in supernatant of HepG2.2.15 cell lines.
    方法脂质体转染pcDNA3.1 Human APOBEC3G-Myc-6Xhis、pcDAN3.1/His-C进入HepG2.2.15细胞,转染后,RT-PCR证实转染基因的表达,Western Blot证实蛋白的表达。
    The amount of HBsAg and HBeAg in the supernatant of HepG2.2.15 cell line transfected with pcDNA3.1 Human APOBEC3G-Myc-6Xhis was apparently decreased by 70.38% and 62.88%,respectively. Conclusion It is powerfully verified that APOBEC3G can inhibit HBV replication in vitro.
    结果APOBEC3G基因与蛋白在HepG2.2.15细胞都有表达,与空质粒转染组相比,pcDNA3.1 Human APOBEC3G-Myc-6Xhis转染组HBsAg含量下降70.38%,HBeAg含量下降62.88%,未转质粒细胞为空白对照组。
    METHODS Natural products are collected to screen in the stable cell line of GLP-1 receptor, and the active component was identified in the PC12 cells.
    方法建立稳定的GLP-1受体激动剂高通量筛选模型,从天然药物中筛选活性成分; 在PC12细胞中验证GLP-1受体激动剂的功能及作用机制。
 

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