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    Detection of cytoplasmic antigens CD_ 3,CD_(22), MPO and it′s clinical significance
    胞浆内抗原CD_3、CD_(22)、MPO的检测方法及临床意义
    MAGE proteins distribute at two types in NSCLC tissues and are cytoplastic antigens.
    MAGE蛋白在NSCLC组织间有两种分布形式,属于胞浆内抗原
    Objective To evaluate the method of marking cytoplasmic antigen ( CD3, CD22, MPO) and their clinical significance.
    目的 探讨和明确检测胞浆内抗原CD3、CD22、MPO的检测方法及其在白血病诊断中的意义。
    Results The B-acute lymphoblastic leukemia( B-ALL) and acute myeloid leukemia (AML) cases were found to be false positive for CD3 ( Becton Dickinson) and negative for CD3 ( Immunotech) .
    结果 急性B淋巴细胞性白血病(B-ALL)、急性髓细胞性白血病(AML)标本使用抗CD3抗体(Becton Dickinson公司)进行胞浆内抗原检测时,出现假阳性的结果;
    Methods The cytoplasmic antigen CyCD79a was detected in 134 patients with AML,31 patients with T-ALL and both CyCD79a and CyCD22 were detected in 83 patients with B-ALL by flow cytometry.
    方法采用直接免疫荧光法对134例急性髓细胞白血病(AML)及31例T细胞型急性淋巴细胞白血病(T-ALL)患者骨髓或外周血进行胞浆内抗原CyCD79a标记染色,83例B-ALL患者同时进行胞浆内抗原CyCD79a和CyCD22标记染色,以CD45/SSC设门法进行三色流式细胞术分析。
    Objective:To explore the examinational method of CyCD 3,CyCD 22 .MPO and the clinical significance.
    目的 :探讨和明确胞浆内抗原 CD3、CD2 2 、MPO的检测方法及其在白血病诊断中的意义。
    Methods Two or one color monoclonal antibodies was used to label whole blood directly, flow cytometry were used to detect, the criteria for classification was according to antibody integral system.
    方法 细胞膜抗原采用全血单克隆抗体直接双标记法 ,胞浆内抗原CyMPO ,CyCD3,CyCD2 2的检测在对细胞膜进行通透性处理后 ,采用单色荧光标记 ,流式细胞仪检测。 分型根据抗体积分系统。
    CyCD 22, CyCD 3 and CyMPO were specifically expressed in B lineage, T lineage and myeloid lineage leukemia, respectively, and the first two could be more sensitively detected than they were on cell membrane surface.
    胞浆内抗原CD2 2 、CD3 及髓过氧化物酶 (MPO)分别特异地表达在B ALL、T ALL及AML ,并比胞膜抗原检测更为敏感。
 

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