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bladder carcinoma cells
    The Study of Tumor Suppression Function of RB1 Gene in Human Bladder Carcinoma Cells in Vitro
    RB_1抑癌基因对人膀胱癌细胞抑癌作用的体外研究
    A study of tumor suppression effect of RB_1 gene on human bladder carcinoma cells in vitro
    RB_1抑癌基因对人膀胱癌细胞抑癌作用的研究
    RETROVIRUS MEDIATED GENE TRANSFER AND EXPRESSION OF HUMAN GM-CSF IN BLADDER CARCINOMA CELLS
    逆转录病毒载体介导人GM-CSF基因在膀胱癌细胞株的转移和表达
    Retrovirus mediated human IL 2 gene transfer in human bladder carcinoma cells
    逆转录病毒介导人白细胞介素2基因在人膀胱癌细胞中的表达
    An experimental study of microwave radiation on the bladder carcinoma cells in vitro
    微波对膀胱癌细胞杀伤的体外实验研究
    After successful cell transfection indicated by GFP expression, GCV and hydroxycamptothecin are respectively added into the cell culture with normal T-24 cells serving as the blank control group. The growth inhibition rate of hunman bladder carcinoma cells in response to HCPT treatment for 72 h and the cell survival rate of 24 h, 48 h and 72 h after transfection with different protocols were observed by MTT assay.
    以正常T-24细胞为空白对照,转染成功后分别加入GCV、GCV+HCPT,采用MTT法测定单纯HCPT作用于人膀胱癌细胞72h细胞增殖抑制率、各组治疗转染后人膀胱细胞24、48、72h细胞存活率;
    Controlled expression and significance of p16 protein in bladder carcinoma cells transfectioned wild type MTS1
    转染野生型MTS1基因的膀胱癌细胞中p16蛋白的可控表达及意义
    The influence of transfection with E1AF on invasive ability of the bladder carcinoma cells
    转染E1AF对膀胱癌细胞侵袭能力的影响
    Study on relationship between expression of nm23 protein and resistance of mitomycin C for human bladder carcinoma cells
    人膀胱癌细胞nm23蛋白表达量与丝裂霉素抗药指数之间的相关性研究
    Inhibiting effect of vascular endothelial growth factor (VEGF) antisense oligonucleotides on VEGF in bladder carcinoma cells
    VEGF反义寡核苷酸抑制人膀胱癌细胞VEGF表达的作用和效果
    Effects of 8-Br-cAMP on the Expression of c-erbB-2 c-myc Gene in Bladder Carcinoma Cells
    8-Br-cAMP对膀胱癌细胞中c-erbB2c-myc表达的影响
    Knockdown of Survivin expression with RNA interference induces bladder carcinoma cells apoptosis
    利用RNA干扰阻抑膀胱癌细胞Survivin基因表达和诱导凋亡
    Methods An eukaryotic expressive vector pcDNA3 E1AF was constructed and introduced into a strain of bladder carcinoma cells BIU 87 with transfection.
    方法 构建真核表达载体pcDNA3 E1AF ,并以此载体转染膀胱癌细胞株BIU 87。
    Mean volume of tumor in nude mice on the 10th day after bladder carcinoma cells transplanted was (40.4±18.2) mm 3 in CTL group and (220.3±31.2) mm 3 in control( P< 0.01),whereas mean survival time was 32.5 days and 16.3 days respectively( P <0.01).
    在体内 ,CTL组和未经处理的对照组比较 ,膀胱癌细胞移植到裸鼠体内的第 10天 ,肿瘤体积分别为 ( 4 0 .4± 18.2 )mm3 和 ( 2 2 0 .3± 3 1.2 )mm3 (P <0 .0 1) ,裸鼠平均生存期分别为 3 2 .5天和 16.3天 (P <0 .0 1)。
    Objective To study the effects of 8-Br-cAMP on the expression of c-erbB-2,c-myc gene and mRNA in bladder carcinoma cells.
    目的探讨环腺苷酸(cAMP)类似物8-溴-环腺苷酸(8-Br-cAMP)对人原代培养的膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达的影响。
    Methods After human bladder carcinoma cells were cultured primarily in the media containing 10-5mol/L of 8-Br-cAMP ,the alteration of expression of c-erbB-2,c-myc gene and mRNA were observed by using immunohistochemistry SP method and in situ hybridization.
    方法原代培养膀胱癌细胞,采用完整细胞免疫组化、原位杂交方法检测膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA的表达。
    Conclusion 8-Br-cAMP could inhibited the expression of c-erbB-2,c-myc and mRNA in bladder carcinoma cells, it indicated that 8-Br-cAMP was an effective inhibitor for mitotic and proliferation of tumor cells.
    结论8-Br-cAMP可抑制膀胱癌细胞中c-erbB-2、c-myc蛋白及mRNA表达,进而抑制肿瘤细胞的分裂和增殖。
    Objective To study the inhibition effect of VEGF expression in human bladder carcinoma cells by ~125I-coupled antisense oligonucleotides (~125I-ASODN).
    目的观察125I偶联VEGF反义寡核苷酸(125I-ASODN)对膀胱癌细胞VEGF表达的抑制情况。
    Methods cell culture of E-J bladder carcinoma cells , they were divided into three groups and were treated with PVP (1%PVP)、ADM、PVP+ADM separately ,12h after the administration of the drug to the cell culture ,cells were washed with and cultured for another 24h , percentage of cell necrosis was assessed with flow cytometry .
    方法E-J膀胱癌细胞体外培养,实验分3组PVP组(1%PVP)、ADM组、PVP+ADM组,药物与细胞培养12h后用生理盐水冲洗再分别培养24h,流式细胞仪检测细胞凋亡的百分数。
    Conclusion PVP could significantly increase the ADM's anti-cancer effect towards E-J bladder carcinoma cells,the mechanism might be the adhesion character of PVP.
    结论PVP在体外显著提高ADM抗E-J膀胱癌细胞效应,其作用机理可能在于PVP的粘附性。
 

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