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fluorescence intensity
    Methods HUVECs were incubated with the calcium ion-sensitive fluorescent indicator fluo-3/AM, and then a laser confocal microscope was applied to measure changes of fluorescence intensity under different agonists to investigate the effects of Breviscapine on intracellular Ca2+ concentration in HUVECs.
    方法采用新一代Ca2+荧光探针Fluo-3/AM标记培养的HUVECs,激光共聚焦显微镜检测细胞胞内钙荧光信号,观察灯盏花素对培养的HUVECs胞内Ca2+水平的调控作用。
    RESULTS ①Fluorescence intensity of intracel lular DCF-DA and myocyte hypertrophy increased by ET-1 in dose-dependent mann er.
    结果 ①ET 1浓度依赖性地使心肌细胞内DCF DA的荧光信号增加和心肌细胞肥大。
    Antioxidant catalase(0 2 U·L -1 ) attenuated the ET-1-induced incre ase of fluorescence intensity of intracellular DCF-DA and myocyte hypertrophy.
    过氧化氢酶 (catalase ,CAT ,0 2U·L- 1 )抑制ET 1 (1× 1 0 - 8mol·L- 1 )诱导的心肌细胞内DCF DA的荧光信号的增加和心肌细胞肥大。
    ②Simvastatin inhibited the increase of fluorescence intensity of intracellular DCF-DA and cardiac myocyte hypertrophy induced by ET-1(10 -8 mol·L -1 )in a dose-dependent manner.
    ②辛伐他汀对ET 1诱导的心肌细胞内DCF DA的荧光信号增强和心肌细胞肥大产生剂量依赖性的抑制作用。
    Methods: Single ventricular myocytes were enzymatically isolated, loaded with fura 2/AM (0.5 μmol/L) for 30 min in dark at room temperature and field stimulated (0.5 Hz, 5 ms), and both myocyte contraction and intracellular fluorescence intensity were simultaneously assessed by a video based motion edge detection system.
    方法常规酶解法分离制备钙耐受心肌细胞; 用Ca2+荧光探剂fura2/AM(0.5μmol/L)负载心肌细胞30min(25℃)后,以含氧台氏液灌流并予电场刺激(0.5Hz,5ms),采用动缘探测系统检测心肌细胞收缩/舒张功能及细胞内荧光信号变化。
    Results PNS (0.05, 0.10, and 0.15 g/L) significantly decreased the protein content, 3H-Leu incorporation rate, and Ca 2+ fluorescence intensity compared with the model group.
    结果与模型组相比,0.05、0.10、0.15g/LPNS可显著降低细胞内蛋白质的量和3H-亮氨酸掺入量,减弱细胞内Ca2+荧光信号
    At the same time, we study influence of irradiation time, PH and fluorescence quencher on fluorescence intensity.
    同时研究了激发时间,溶液PH值和荧光猝灭剂对荧光信号的影响。 根据农药的荧光特性,设计了荧光激发、收集和传输的光学系统。
    Owing to the disturbances of non-specific fluorescence, the cluster of G2M phase cells was always higher than that of G1 phase cells in BrdUrd/DNA bivariate distribution, and the peak of BrdUrd negative cells indicated asymmetrical display and occasionally yielded a small splitted peak in the distribution of BrdUrd. fluorescence intensity.
    由于非特异荧光信号的存在与干扰,在BrdU_1d/DNA二维分布图上G2M细胞簇总是高于G1细胞簇,并且在BrdUrd强度分布图上BrdUrd阴性细胞峰呈不对称性或出现另一小蜂,这造成计算机数据处理困难.
    The results showed that the fluorescence intensity increased with the increasing concentration of enterotoxins in samples. But if the concentrations were too high, the fluorescence intensity did not increase any more. The detection limits of SEA, SEB, SEC are 0 01μg/ml, 0 01μg/ml and 0 1μg/ml respectively.
    对影响免疫芯片检测效果的条件进行了优化 ,按优化后的条件进行SEA、SEB、SEC的检测 ,结果表明荧光信号强度随待测物浓度的增加而增加 ,当浓度高于一定值时 ,荧光信号强度趋于一定值 ,本法最低可检测 0 0 1 μg ml的SEA和SEB及 0 1 μg ml的SEC。
    And the resonance scattering intensity of AgCl, AgBr and AgI systems is 110, 130 and 80 times of the fluorescence intensity, respectively. That is, the relationship between fluorescence and resonance scattering is exist.
    卤化银纳米微粒体系的浓度对共振散射信号的影响与浓度对荧光强度的影响一致 ,AgCl ,AgBr和AgI体系的共振散射光信号强度分别约为荧光信号的 110 ,13 0和80倍 ,即荧光与共振散射之间存在相关性 .
    The results indicated that the fluorescence intensity was increased along with the decreasing concentration of samples,when the concentration lies in 0.001~0.4μg/ml,it takes on a linear trend. The detection range is from 1~0.001μg/ml. ;
    实验结果表明荧光信号随待测物浓度的降低而增强 ,待测物浓度在 0 0 0 1~ 0 4μg ml的范围内有较好的线性趋势 ,检测范围为 1~ 0 0 0 1 μg ml。
    The concentration of protein in the spotting solution significantly affected the fluorescence intensity.
    点样量影响荧光信号的强度。
    The results demonstrate that fluorescence intensity increased along with the decreasing concentration of analytes. And then, linear trend obtained. The limit of detection of atrazine and papaverine is 0.001 and 0.01 mg/L respectively.
    并对阿特拉津及罂粟碱进行了定性、定量实验,结果表明:荧光信号强度随待测物浓度的降低而增强,有一定的线性趋势,阿特拉津检出限为0. 001mg/L,罂粟碱检出限为0. 01mg/L。
    Employing a frequency-doubled dye laser with wavelength of 283.553nm as exciting laser,the fluorescence intensity of hydroxyl is nearly indirect proportion to the mole fraction of hydroxyl.
    选择波长为283.553nm的染料激光倍频光作为激发光,可近似认为获得的OH基荧光信号强度与OH基的摩尔分数成正比。
    Atrazine's fluorescence intensity was best when the antibody concentration was reached to 0.254 mg/ml,and 3% BSA(pH 7.2~9.0) was used as buffer blocking for 30~60 minutes.
    当pH 7.2~7.4之间、抗原抗体反应时间120 min、反应温度为37℃和适度漂洗时能获得理想荧光信号
 

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