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fluorescent signals
    Methods mRNA was extracted and purified from the lung of the mouse with LA795 lung metastasis, hybridized respectively on 4 096-gene chip. cDNA microarray was scanned for the fluorescent signals and analyzing difference expression.
    方法 以LA795小鼠肺腺癌肺转移组织 ,制备mRNA探针 ,应用含有 40 96条鼠基因的cDNA表达谱芯片 ,用ScanArray 3 0 0 0扫描仪扫描芯片荧光信号图像 ,分析肿瘤组织和康莱特治疗后肿瘤组织中差异表达基因。
    Methods The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol,then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently,the cDNA probes were hybridized to the Mouse 40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed.
    方法 NP染毒F0亲代母鼠 ,分别提取NP组和对照组F1子代 2日龄雄性大鼠的脑组织mRNA ,分别用cy5和cy3逆转录标记 ,与Mouse4 0S基因表达谱芯片杂交 ,并对cy3、cy5荧光信号做扫描分析。
    Methods The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently,the cDNA probes were hybridized to the Mouse40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scaned and analyzed.
    方法NP染毒F0亲代母鼠,分别提取NP组和对照组F1代雄性2d龄大鼠的脑组织mRNA,分别用cy5和cy3逆转录标记,与BiostarR-40S基因表达谱芯片杂交,并对cy3、cy5荧光信号做扫描分析。
    Methods:The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol,then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently,the cDNA probes were hybridized to the BiostarR-40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scaned and analyzed.
    方法:NP染毒F0亲代母鼠,分别提取NP组和对照组F1代雄性2日龄大鼠的脑组织mRNA,分别用cy5和cy3逆转录标记,与BiostarR-40S基因表达谱芯片杂交,并对cy3、cy5荧光信号做扫描分析。
    Objective To study the integration of HBV on hepatocellular chromosome of the patients with chronic hepatitis B.Methods Chromosome fluoresent in situ hybridization(FISH) was done with dig-labeled HBV probe in 16 patients with chronic hepatitis B.Results Green fluorescent signals were seen in 5 patients(31.25%),the integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.
    方法选择我院慢性乙型肝炎患者16例,采用荧光原位杂交(FISH)技术制备肝细胞染色体标本,以地高辛标记的HBV探针进行原位杂交。 结果16例患者中,5例(31.25%)肝细胞分裂期染色体上可观察到绿色荧光信号,HBV呈低频率整合(1.00%~1.50%),整合位点呈随机分布。
    Chromosome in-situ hybridization was done with Dig-labeled HBV probe. Green fluorescent signals were seen in 5 patients specimens(31.25%). The integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.
    16例患者中,有5例患者的肝细胞分裂期染色体上可观察到绿色荧光信号,占31.25%,HBV呈低频率整合(1.00%~1.50%),整合位点呈随机分布。
    In order to decrease noise among fluorescent signals,cutoff value was calculated,and results was showed in the form of(Ssignal-Nnoise).
    为便于结果判定,确定截断(cutoff)值,并以Ssignal与Nnoise差值的形式消除荧光信号中的噪声。
    After high-stringent washing, the cDNA microarray was scanned for fluorescent signals and showed differences between two tissuses.
    严格洗片后扫描其上的荧光信号,然后分析比较两种组织间的差异。
    And 1 hour later, fluorescent signals became bright.
    用阳离子脂质体转染荧光标记的DecoyODNs,半小时细胞内即有少量荧光,1小时显微镜下可见所有的细胞内均有荧光信号
    Results When the DNA concentration was low, the nucleotide curve showed low ratio of signal to noise, even there were no fluorescent signals.
    结果 测序模板浓度过低 ,则核苷酸曲线的信∶噪比小 ,甚至不出现荧光信号
    After washing,the cDNA microarray was scanned for the fluorescent signals and showed differences between 2 tissues.
    经严格洗片后用扫描仪扫描芯片荧光信号图像 ,计算机分析后比较两种组织中差异表达的基因。
    After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed the gene expression differences between the young and the aged.
    通过扫描仪扫描芯片荧光信号和计算机分析,比较老年组和青年组大鼠脾脏的基因表达水平的差异。 同步检测老年组大鼠和青年组大鼠的心、肝、肾和血液中的与自由基相关的生化指标。
    Results Green fluorescent signals were seen in 4 patient specimens ( 2.94%). All four patients possessed all positive HBsAg and HBeAg mothers. The integration sites were present in low frequency and randomly distributied.
    结果  136例中 ,有 4例患者的外周血淋巴细胞分裂期染色体上可观察到绿色荧光信号 ,占 2 .94 % ,均为双阳组孕妇 ,HBV呈低频率整合 (1.0 0 %~ 1.6 7% ) ,整合位点呈随机分布。
    The chips were scanned by ScanArray 4000 laser scanner. The acquired fluorescent signals were analyzed by GenPix Pro 3.0 software. Bioinformatical analysis of those differentially expressed genes was performed.
    以ScanArray 40 0 0扫描仪扫描芯片 ,以GenPixPro 3 0软件分析荧光信号 ,然后对差异表达的基因进行生物学信息分析。
    After high stringent washing,the cDNA microarray was scanned for fluorescent signals and the differences between the two tissuses were compared.
    经严格洗片后扫描芯片荧光信号图象 ,计算机分析后比较 2种组织中差异表达的基因。
    The chips were scanned by ScanArray 4 000 laser scanner. The acquired fluorescent signals were analyzed by GenPix Pro 3.0 software. Bioinformatical analysis of those differentially expressed genes had been performed.
    以ScanArray 40 0 0扫描仪扫描芯片 ,以GenPixPro 3 0软件分析荧光信号 ,对三组差异表达的基因进行生物学信息分析。
    Results:Only V. parahaemolyticus strains generated fluorescent signals, and no cross-reaction was observed with other 11 bacterias. The sensitivity achieved was 69cfu /ml or 6cfu/PCR reaction.
    结果 :检测 12种细菌 ,只有副溶血弧菌有荧光信号 ,与其他细菌无交叉反应 ,DNA灵敏度为 16 6 .6 fg/ μl,菌液灵敏度为 6 9cfu/ ml或 6 cfu/ PCR反应体系。
    Methods The mRNA were extracted respectively from the brain of 2-day-old F1 generration rats whose F0 male generation were tested with and without nonylphend, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently, the cDNA probes were hybridized to the Mouse40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scaned and analyzed.
    方法 NP染毒F0亲代母鼠 ,分别提取NP组和对照组F1子代雄性 2日龄大鼠的脑组织mR NA ,分别用cy5和cy3逆转录标记 ,与Mouse4 0S基因表达谱芯片杂交 ,并对cy3、cy5荧光信号做扫描分析。
    〔Methods〕 The changes of blank fluorescent signals in potassium ferrocyanide solution were observed. Meanwhile,the reasons for the blank fluorescent signals and methods for its decrement were determined.
    〔方法〕观察铁氰化钾空白溶液荧光信号的变化 ,研究空白荧光信号产生的原因及降低的方法。
    〔Results〕 Impure lead in potassium ferrocyanide was one of the reasons for fluorescent signals of blank solution and it could be separated by activated carbon in the solution.
    〔结果〕证实铁氰化钾试剂中杂质铅是导致试剂空白溶液荧光信号产生的原因之一。
 

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