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    Extracting high-quality RNA from common grape hyacinth flowers using common methods is difficult because of high levels of polysaccharide in flowers. Total RNA was isolated from common grape hyacinth with modified CTAB method and SDS method. UV-Spectroscopic analysis showed that A(260)/A(280)= 1.8~2.0,28S,18SrRNA were all clear in electrophoresis picture.
    在普通CTAB法与SDS法提取RNA的基础上,从去除多糖和DNA污染两个方面进行优化,筛选出适于富含多糖类葡萄风信子花瓣RNA提取的方法,经检测OD260/280值在1.8~2.0之间,电泳28S条带和18SrRNA条带都很清亮,比值约1.5。
    Research of Total RNA Extraction Methods from Polysaccharideriched Petals of Common Grape Hyacinth
    富含多糖葡萄风信子花瓣总RNA提取方法研究
    Study on Muscari botryoides Mill Leaf and Bulb Adventitious Bud Regenerating System
    葡萄风信子叶片、鳞茎不定芽再生体系的建立
    Propagation and Flower Control of Muscari armenicum Leichtlin
    葡萄风信子种球繁殖与花期调控
    Chilling the bulbs at 5 ℃ for 7 weeks,8 weeks,9 weeks,10 weeks,11 weeks showed that had good effects on promoting efflorescence,the longer the bulbs treated,the earlier the bulbs blossomed.
    在5℃下,将种球分别冷藏7周、8周、9周、10周、11周,结果表明种球冷藏对葡萄风信子开花有促成作用,冷藏时间越长,开花越早。
    The study is benefit to bulb production and flower supply.
    研究可对葡萄风信子种球生产与商品花节日上市提供技术指导。
 

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