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    STUDY ON CHROMATOGRAPHY OF INTERFERON-R AND IL-2 BY USING BLUE SEPHAROSE CL-6B
    用蓝色琼脂糖CL-6B配体亲和层析IFN-γ和IL-2的实验研究
    RESULTS: ①Observation of growth status of umbilical mesenchymal stem cells and expression of LacZ report gene: After transfection, cells of the transfection group showed no obviously proliferation. 72 hours later, a great deal cells synthesized galactosidase and were X-gal stained into blue with fusiform shape;
    结果:①脐血间质干细胞生长情况及LacZ报告基因表达的检测:转染组病毒基因转染后未再见到明显的细胞增殖,转染72h后大部分细胞表达LacZ基因并合成半乳糖苷酶,X-gal染色呈蓝色,长梭形;
    After extracting and sequencing of plasmids from blue colonies, we underwent sequence analysis by bioinformatics.
    挑选蓝色克隆,提取此酵母克隆的质粒转化大肠杆菌提取质粒DNA后进行测序,然后进行生物信息学分析。
    1. We chose 15 fresh adult knee samples without obvious genual diseases ( 5 examples of extended position,5 examples of flexed 45 degrees position, 5 examples of flexed 90 degrees position ), embed the samples of different functional positions with 5% blue glutin, and obtained 0.5mm sample by mill cuts technology at low temperature condition.
    1.选用无明显关节病变的新鲜成年膝关节标本15例(全伸位、屈曲45o及90o三个功能位各5例),将各功能位标本用5%蓝色明胶包埋,低温条件下对标本进行层厚为0.5mm的薄层铣切。
    In this paper,we reported the resu-ltes of chromatography of purifyinginterferon-Y and IL-2 with bluesepharose CL-6B.Interferon-γ wasproduced from the spleen cells to beinduced by PWM,then it was puri-fied by blue sepharose column CL-6B.Theinterferon-γ and IL-2 can beeluted into two fractions.
    由美洲商陆(PWM)诱导人脾细胞产生γ干扰素(IFN—γ),再经蓝色琼脂糖CL-6B线性梯度柱层析、一步纯化可将IFN-γ和IL-2洗脱成2个组份。
    In this study, toluidine blue O stain was conducted in the lung smears of rats and mice
    本研究以可的松诱发卡氏肺孢子虫感染的大鼠与小鼠,制备肺涂片进行甲苯胺蓝染色。 所有的卡氏肺孢子虫包囊均染成深紫红色,与蓝色背景易于区别。
    In addition, the transduced cells could be stained by X-gal into blue.
    同时,转导阳性细胞能够被X-gal染成蓝色.
    Result:Cells infected by Ad-LacZ were dyed blue by X-gal. Cells infected by Ad-CD were obviously killed after administration of 5-FC.
    结果 :感染Ad -LacZ的细胞可被X - gal染成蓝色 ,感染Ad -CD的细胞予前药 5 -FC可见肿瘤细胞杀伤效果明显。
    Results In low concentration CO and hemin group, typical DNA ladder bands and apoptotic cells were found in lung tissues, and the expressions of Fas, FasL, bax, and bcl 2 mRNA were increased in the epithelial cells of alveoli, vascular and bronchial walls. And the expression of bcl 2 mRNA was decreased than in hypoxic group.
    结果 低浓度CO组和血晶素组肺组织有典型的DNA梯带 ,bcl 2、bax、Fas、FasL免疫组化显示的表达呈强棕黄色 ,比低氧组均显著升高 ,原位杂交可见 ,低浓度CO组和血晶素组肺内支气管和小血管壁细胞胞浆呈强的紫蓝色 ,bcl 2表达呈棕黄色 ,比低氧组降低 ,原位杂交可见呈紫蓝色
    Results:The titers of the unamplified library and the amplified library are 2 0×10 7pfu/mL and 1 75×10 9pfu/mL, respectively.
    结果 :经检测 ,未扩增文库滴度达 2 0× 10 7pfu mL ,重组效率在 10 -4 稀释度时每块平板约 2 10~ 2 5 5个噬菌斑中均未发现任何蓝色噬斑 ,扩增文库滴度达 1 75× 10 9pfu mL ;
    The stronger ALPase blue reactions present in blood vessels, and the stronger 5' Nase brown reactions appear in lymphatic vessel like structure.
    血管呈现ALPase强阳性蓝色反应 ,淋巴管表现 5’ Nase强阳性褐色反应。
    Biotin labelled 13q 14.3 specific probe and Digoxigenin labeled 14q 11.1 specific probe were used for in situ hybridization of sperm specimens in 2 Robertsonian translocation carriers.
    结果 :在显微镜下可见精子头部有以Biotin标记的 13q 14 .3特异性探针显示 1个绿色杂交信号 ,以Digoxi genin标记的 14 q11.1特异性探针显示 1个红色杂交信号 ,间期核背景经DAPI复染显示蓝色 ;
    ② In X Gal assay, co transformants of FLN C and α1A AR CT turned blue at 6 h, while control yeast clone did not;
    ②X Gal定性分析中 ,FLNC与α1A AR CT的共转子菌落 6h即呈现蓝色 ,而对照菌落颜色无变化 ;
    After extracting and sequencing of plasmids from blue colonies, we underwent sequence analysis by bioinformatics.
    挑选蓝色克隆,提取此酵母克隆的质粒转化大肠杆菌提取质粒DNA后进行测序,然后进行生物信息学分析.
    VIP(1×10~ -8 mol/L)decreased the ratio of granulocyte (CD13~ + ) and monocytes(CD14~ + )in human CD34~ + cell population (P<0.05).
    VIP(1×10-8mol/L)作用于人CD34+细胞14d后,粒系(CD13+蓝色)及单核系(CD14+)细胞百分比均明显下降(P<0·05)。
    on the other hand, Mac-1 could cluster after combined with ligand ICAM-1 on activated endothelial cells through the outside-in signaling pathway.
    利用前面构建完成并表达的CD11b-BFP与YFP-CD18融合蛋白在380nm激发下可见蓝色荧光,在514nm激发下发生黄色荧光这一特性,用于研究Mac-1的走向与归宿是比较理想的。
    (2) Using both upper and lower limbs of a fresh adult cadaver,inject red gelatin into femoral,forward and backward injecting the blue gelatin withhigh pressure into femoral vein with formalin preventing rot.
    (2)采用新鲜成人尸体上、下肢标本,于股动脉灌注红色乳胶,足背静脉网顺、逆行及股静脉加压灌注蓝色乳胶,福尔马林溶液防腐。
    (4) Deep freezed right lower limb sample,injecting red gelatin into femoral artery,forward and backward injecting blue latex with barium sulfate into femoral vein and great saphenous vein,cuting flap after dealing with formalin and get x-ray photos.
    (4)右下肢冷藏标本,于股动脉灌注红色乳胶,再于股静脉及大隐静脉顺、逆行灌注含硫酸钡混悬液之蓝色乳胶,福尔马林防腐,然后切取皮瓣,摄取x线片。
 

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