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the expression
    Studies on the Expression of HSPs in Tissues of the Stressed Cattle
    应激肉牛几种组织中HSPs表达的研究
    Expression of Nucleocapsid Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus in Soluble Form and Development of an Indirect ELISA for the Detection of Viral Antibodies with the Expression Product
    猪繁殖与呼吸综合征病毒核衣壳蛋白基因的高效可溶性表达和检测血清抗体的间接ELISA方法的建立
    Study on the Regulation of FSH on the Expression of GDNF of Piglet Testis Sertoli Cell
    FSH对仔猪睾丸支持细胞GDNF表达调节的研究
    Study on the Expression of Ovine Sperm Membrane Protein Genes
    绵羊精子膜蛋白基因的表达研究
    Inhibition of Hog Cholera Virus by the Expression of Spe-cific Antisense RNA in Cells
    体外培养细胞中特异反义RNA表达对猪瘟病毒增殖的抑制作用
    Effects of Salmonella typhimurium on the Expression of MHC-1,MHC-2,ICAM-1 and CD4 of Lymphocytes in Mice
    鼠伤寒沙门氏菌对鼠淋巴细胞MHC-1、MHC-2、ICAM-1和CD4表达的影响
    the methionine was added at levels of 0, 20, 40, 60, 80 or 100 μg/mL. The expression of casein αs1 gene was enhanced with increasing addition levels of methionine from 0 to 60 μg/mL and then decreased thereafter. The estimated optimal level of methionine was at 57 μg/mL.
    蛋氨酸添加水平为0、20、40、60、80和100μg/mL,结果表明,添加蛋氨酸的浓度为0~60μg/mL时酪蛋白αs1基因表达随蛋氨酸浓度的增加而增强,60~100μg/mL时酪蛋白αs1随蛋氨酸浓度的增加而减弱,添加蛋氨酸最佳浓度为57μg/mL,此时基因表达最强。
    The S1 gene of Infectious bronchitis virus(IBV) was segmented and cloned into the expression vector pGEX-6p-1 by gene recombination technology. The glutathiones transferase ( GST) fusion proteins were induced by IPTG.
    利用基因重组技术将鸡传染性支气管炎病毒(IBV)S1基因分段(A和B段)克隆到pGEX-6p-1载体中,用IPTG诱导表达
    The seven plasmids were transfected into SP2/0 cell in vitro to detect the expression of target genes.
    转染SP2/0细胞,检测目的基因的表达
    The expression of CAT1 mRNA in colorectum was very significantly higher than that of duodenum,jejunum and ileum(P<0.01). Expression abundance in ileum was higher than in jejunum significantly(P<0.01) and higher than duodenum by 27.9 %(P=0.111).
    结直肠CAT1 mRNA表达丰度极显著高于十二指肠、空肠和回肠(P<0.01),回肠极显著高于空肠(P<0.01),高出十二指肠27.9%(P=0.111)。
    On the whole,the expression level of LPL mRNA in muscle of LW tended to be higher than that of LL(P>0.05).
    总体上莱芜猪肌肉组织LPL基因表达量略高于鲁莱黑猪(P>0.05)。
    The expression level of the nucleoprotein gene in pET-32a(+)(30.4%) was higher than that in pET-28a(+)(19.4%). The yield of the highly purified nucleoprotein was about 8.45 mg in pET-28a(+) and 13.6 mg in pET-32a(+) per liter bacterial culture,respectively.
    结果显示,核蛋白在pET-32a(+)中的表达量(30.4%)高于在pET-28a(+)中的表达量(19.4%),培养物中的高纯度核蛋白产量分别为13.6和8.45 mg/L。
    It promoted the increase of IGF-Ⅰ. IGF-Ⅰcaused the expression of KAP3.2 and KAP6-1 to significantly increase both in plucked and shaved sheepskin. The pattern of expression changes of KAP3.2 and KAP6-1 were similar.
    IGF-Ⅰ对拔毛皮肤和剃毛皮肤中的KAP3.2和KAP6-1的表达都能显著提高,且表达模式也基本一致,KAP3.2的表达早于KAP6-1的表达
    【Conclusion】The results above indicate that IGF-1 has no effect on the expression of GHR and IGF-ⅠR,and had a stimulative effect on that of KAP3.2 and KAP6-1.But the specific expression order of KAP3.2 and KAP6-1 were reversed. Further study is needed to determine the reason that extrinsic IGF-Ⅰhas no effect on hair follicle growth in vivo.
    结论IGF-Ⅰ对绵羊皮肤中的GHR和IGF-ⅠR的表达均无显著影响,对KAP3.2和KAP6-1的表达能显著提高,但呈现出颠倒的特定时序性,这是否是外源性IGF-Ⅰ不能促进体内毛囊生长的原因所在,有待进一步研究。
    Methods The M gene of PRRSV was amplified with RT-PCR and cloned into the plasmid pcDNA3. The constructed plasmid pcDNA3-M was transfected into NIH/3T3 cells and screened by G418. Results Anti-G418 NIH/3T3 cells were obtained and the expression of M protein in anti-G418 NIH/3T3 cells was proved by IFA.
    用表达M蛋白的重组腺病毒rAd-M免疫小鼠,用表达M蛋白的NIH/3T3细胞和乳酸脱氢酶法检测PRRSV特异性细胞毒T淋巴细胞的杀伤效应。 结果获得有G418抗性的NIH/3T3/M细胞克隆;
    To investigate the relationship between expression of PTEN and VEGF and clinicopathological features in ca-nine mammary gland tumor,the expression levels of PTEN and VEGF protein were assessed in32cases of canine mam-mary gland tumors tissues and6cases of normal mammary gland tissues using SP immunohistochemical method.
    为探讨犬的乳腺肿瘤组织中PTEN和VEGF蛋白表达与临床病理学特征关系的相关性,采用免疫组织化学SP染色法检测了32例乳腺肿瘤组织及6例正常乳腺组织中上述2种蛋白的表达
    Conclusion The expression cassette OAAT for multiple-epitope antigen of FMDV was successfully designed,and trivalent FMDV DNA vaccine pVAX1-OAAT was successfully constructed.
    结论已成功设计FMDV复合多表位基因工程疫苗表达盒OAAT,并构建了三价口蹄疫核酸疫苗pVAX1-OAAT。
    The expression of the E2 and GP5 in rPRV-E2-GP5 infected cells was determined by Western blot and immunofluorescence assay (IFA).
    经Westernblot、间接免疫荧光试验证实E2、GP5基因在重组病毒感染细胞中获得了表达
    The SSTR2 mRNA expression abundance of AA chicken on 30 d was significantly higher than that on other time points(P<0.05) while the expression abundance had no significant difference on 2 d,16 d,44 d and 58 d(P>0.05).
    AA肉鸡30d的SSTR2 mRNA表达丰度显著高于其他日龄(P<0.05); 2d、16d、44d和58d差异不显著(P>0.05)。
    Recombinant baculovirus, rBac-PoIFN-γ, was generated for expressing PoIFN-γ, by transfecting rBacmid-PoIFN-γ with Cellfectin Reagent into sf9 insect cells. The expression of PoIFN-γ in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA.
    以抗PoIFN-γ单克隆抗体为一抗进行Western blot、间接免疫荧光(IFA)及间接ELISA检测,结果表明PoIFN-γ在重组杆状病毒rBac-PoIFN-γ感染的昆虫细胞中获得正确表达
 

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