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    Meanwhile,a preliminary investigation of the optimal fermentation condition,enzyme production regularity and detection system of the activity of xylanase of the bacteria was performed with single factor method,multiple factor combinative method and DNS method,respectively.
    同时,采用单因子和多因子相结合的方法及DNS法,对该菌株的适宜发酵条件和产酶规律及木聚糖酶活检测体系进行了初步研究。
    (2) the optimum conditions in the spinner flask were: soybean meal 15%, glucose 1%~4%, MgCl2 0.02%, K2HPO3 0.01%, pH 6-7, granularity of the soybean meal < 40 holes;
    (2)摇瓶优化的适宜发酵条件为:豆粕15%,葡萄糖1%~4%,氯化镁0.02%,磷酸氢二钾0.01%,初始pH为6~7,豆粕粒度<40目;
    The optimum temperature and initial pH were 29℃ and 4.6, respectively.
    实验结果表明:该菌株的适宜发酵条件为温度30℃、初始pH 4.6;
    The results showed that the optimum conditions of producing cellulase were: under the above conditions of wheat bran and corn stalk ratio was 1:1, water content ratio was 1:2, through the analysis of factors and levels of orthogonal experiment, the temperature was 28℃, the initial pH was 5.5 and grown 72h the activities of FPase in the fermented was 271.363/u, the activities of CMCase in the fermented was 467.700/u.
    试验结果表明,里氏木霉LW1的适宜发酵条件为:在秸秆:麦麸=1∶1,料水比为1∶2的前提条件下,通过正交实验分析,在培养温度28℃,发酵周期为72h,起始pH5.5时产酶活力最高。
    The medium for fermentation consisted of 1% tryptone, 0.5% yeast extract, 5% sweet potato meal, 0.4% Na2HPO4 · 12H2O, 0.03% KH2PO4, 0.1% Na2CO3. The strain produced maximux alkaline protease activity after growth at 42℃ for 44-48 h on a rotary shaker.
    适宜的发酵条件:培养基(%)胰蛋白胨1,酵母膏0.5,玉米粉5,Na_2HP0_4·12H_20 0.4,KH_2P0_4 0.03,Na_2CO_3 0.1,自然pH。
    The optimum fermentative condition was 30℃-65h or 32℃ -55h.
    适宜的发酵条件是30℃-65h或对32℃-55h。
    The optimum fermentative condition was 28℃—55hr or 30℃-65hr.
    适宜的发酵条件是28℃—55h或30℃—65h。
    The suitable cultare conditions are as follows: pH5, inoculate 5%, the seed period is five days and the cultural period is four days.
    白背木耳的适宜发酵条件为:pH为5,移种量为5%,种子周期5d(天),发酵周期4d。
    Suitable fermentation conditions for the chicken excrement using EM were found to be: 50% water content in chicken excrement, 3.5%— 5.0% concentration of EM, temperature of exceeding 25℃ and reaction periods of 3—7 days.
    有效微生物菌群处理鸡粪的适宜发酵条件为:采用自研有效微生物菌群,发酵鸡粪含水量 50%,有效微生物菌群的发酵浓度 3.5%— 5.0% ,发酵温度 25℃以上,发酵时间3—7d。
    Suitable medium contained 6% bean oil as ca rbon source,3 5g/L NaNO 3 as nitrogen source,0 75g/L yeast extract and some i no rganic salts. The fermentation temperature of 30℃,initial pH of 8 5,strring rev olution of 150~240r/min and fermentation period of 96h proved to be optimal.
    适宜发酵条件为 :培养基由 6 %豆油、 3 5g/LNaNO3、 0 75g/L酵母膏以及一定量的无机盐组成 ,发酵温度 3 0℃ ,初始pH8 5 ,搅拌转数 1 5 0~ 2 4 0r/min ,发酵周期 96h。
    Optimal culture conditions in a 3 L fermenter were: stirring speed 300 r/min,aeration 0.3 L/(L·min),and culture temperature 37 ℃. Neutral and alkali cellulase activities detected from the fermentation broth were 257.6 U/mL and 125.6 U/mL,respectively. The enzyme had a good application potential.
    3L发酵罐中的适宜发酵条件为 :搅拌速度 30 0r/min ,通气量 0 .3L/(L·min) ,培养温度 37℃ ,中性和碱性纤维素酶活力分别达到了2 57.6和 12 5.6U/mL ,显示了良好的工业应用前景。
    The results showed that the suitable culture conditions were as follows:temperature 25℃,pH5.5~6.0,stirring speed150180r/min,times 4~5d.
    结果表明:适宜发酵条件为:培养温度24-26℃,培养基起始pH5.5-6.0,振荡频率150-180r/min,培养时间4-5d,菌丝生长最旺。
    the product was obtained under the following conditions:29℃,200r/min and5d fermentation,with some enzymes activity(dry weight ):proteinase3620u/g(dry weight ),cellulase432u/g and saccharifying en-zyme 203u/g,respectively,and the amount of real protein increased by12.1%.
    由适宜的发酵条件,经29℃,200r/min发酵5d得到的产品,具有多种酶活性(以干基计):酸性蛋白酶酶活力3620u/g,纤维素酶活力432u/g和糖化酶活力203u/g,真蛋白质含量(以干基计)提高12.1%。
    The results showed that the optimal culture conditions were 0.3%glucose, 0.5% peptone ,0.3%immersed beefcream, 0.1% NaCl and 0.1% (NH4)2SO4, pH7.0, at 28℃ for 48h.
    结果表明该拮抗菌适宜发酵条件为:葡萄糖0.3%、蛋白胨0.5%、牛肉浸膏 0.3%、NaCl 0.1%、(NH4)2SO40.1%、pH 7.0,培养温度为28℃,时间48h。
    The results show that the optimum conditions are as follows:the mass fraction of methanol in culture solution 3.5%,initial pH 5.0~6.0,inoculum quantity 5%,culture temperature 30℃,and shaking-flask speed 100 r/min.
    试验得出巴氏醋酸杆菌的适宜发酵条件为:发酵培养基中甲醇质量分数为3 5%左右,发酵培养基初始pH为5 0~6 0,接种量为5%,发酵培养温度为30℃,振荡器转速为100r/min。
    Suitable conditions for the highest yield(8.93?g/L) were as follow: 6% plant oil, 0.2% urea with certain amount of inorganic salts. Fermentation temperature of 34℃, initial pH of 8, stirring revolution of 200~240 ? r/min and fermentation period of 2d were used in our study.
    并对其摇瓶发酵工艺进行了初步研究,较高产量(893g/L)的适宜发酵条件为植物油6%,尿素02%,一定量的无机盐,34℃,初始pH值为8,搅拌转速200~240r/min,发酵周期2d。
 

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