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    Effects of AMF inoculated in different substrates on growth of tomato and activity of PAL and PPO
    不同基质接种丛枝菌根真菌对番茄生长及PAL、PPO酶活的影响
    The results showed that: under high salt stress, POD, CAT and BADH activities rose with the increasing of salt concentration; under the same concentration, enzyme activities of Sanfeng gourd were the highest, 53.5%, 98.9% and 31.6% higher than those of wild watermelon respectively;
    研究结果证实,高盐胁迫下,叶片POD、CAT、BADH酶活性均随盐浓度的增加而升高,相同盐浓度下三丰瓠瓜各种酶活性最高,三种酶活性比盐敏感的野生西瓜分别高出达53.5%、98.9%、31.6%;
    Then, two tobacco plastid expression vectors and two lettuce plastid expression vectors were constructed. They contained the homologous fragments of the plastid genomes respectively, the foreign gene (GUS or NK) expression cassette controled by T7 promoter and the expression cassette of the marker gene of BADH.
    其次,构建了两个烟草叶绿体表达载体和两个莴苣叶绿体表达载体,它们分别包括烟草和莴苣的叶绿体同源片段、不同外源基因(GUS和NK)的含T7启动子的表达盒以及筛选标记基因BADH的叶绿体表达盒,并对所构建载体中的BADH表达盒在大肠杆菌中的表达进行了酶活检测。
    Activity of SOD in treatment 2 is 8.47% lower than treatment 1’s, and treatment 3’s is 5.92% lower than treatment 1’s.
    与处理1 比较,处理2 超氧化物歧化酶的活性比处理1 减少了8.47%,处理3 比处理1 减少了5.92%。
    Different degree of saturation of (NH_4)_2SO_4 saltingout the fermentation liquor resulted that the inhibition zone of crude protein antagonistic to pathogen Verticilliumdanliae is the best when the degree of saturation is 70%. We can not measure chitinase activity from the fermentation liquor, and the 0-1, 3-glucanase activity doesn't need inducement.
    发酵液分别用10%~100%饱和度(NH_4)_2SO_4盐析,(NH_4)_2SO_4饱和度为70%时,所沉淀出来的粗蛋白对黄萎病原菌抑菌圈最大,发酵液未能够检测到几丁质酶酶活,β-1,3-葡聚糖酶活性不需要诱导。
    2. NaH2PO4、KCl and MgCl2 could increase the activities of PM protein overall kinases, which had evident relationship with ATPases activities.
    2. NaH2PO4、KCl和MgCl2可提高质膜蛋白总激酶的活性,且激酶活性与质膜ATPases酶活的变化间有显著的相关性。
    2. To research the efficiency of RNAi vector , we constructed a plant expression vectors ( pBI121-F) with a Maasr1 gene and a gus gene.
    构建了一个含有Maasr1基因及Gus的植物表达载体pBI121-F,与上述两个RNAi植物表达载体分别进行共转化,通过GUS组织化学染色及酶活测定发现,两个RNAi表达载体都可以抑制Maasr1基因的表达。
    Mutant HCA15 which has 2.86 and 2.51 times avicelase and Cx of Pleurotus sajor-caju 5·01 is obtained by treating the sporidium with NTG and irradiating monokaryon and dikaryon mycelium with UV. The enzyme activity of HCA15 is high in following conditions: inoculating it in the medium cataining cotton-seed-shell meal、urea.
    以漏斗状侧耳5·01为出发菌株,用NTG和UV依次对孢子、单核菌丝和双核菌丝进行处理,获得了微晶纤维素酶活和羧甲基纤维素酶活分别是出发菌株2.86倍和2.51倍的突变株HCA15。
    sidase for L. edodes breeding. "Hybrid 26" was a new strain bearing A14B13×A1B2 as genetic markers, endo-cellulase activity 45 and exo-ceilobiase activity 45 ("Congo-red" assay) as biochemical markers.
    香杂26号是带有种质标记的新菌株,遗传标记:A_(14)B_(13)×A_1B_2,生化标记:内源纤维素酶酶活:45mm及外源纤维二糖酶酶活:45mm。
    When the extraction buffer at pH 7.0 was used, the activities of the proteinase were the highest at pH 5.5~6.0.Thus, the former hypothesis of proteinases could not be used to explain the decrease of proteins extracted by pH 8.3 buffer.
    研究表明,银杏叶片蛋白水解酶用pH7.0缓冲液提取时活性最高,酶活最适pH5.5~6.0,因而不能解释pH8.3缓冲液提取出的蛋白质含量下降的原因。
    The maximum biomass was 22 12 g DW/L while the maximum total SOD activity was 10 81×10 4 U/L by selecting garlic cell line after 18 days' suspension culture.
    DW/ L及 1 0 .81× 1 0 4 U/ L,最大单位细胞酶活为 679.67U/ g.
    The optimal conditions for βglucosidase were pH 6.6 at 60 ℃, the enzyme was stable over a pH range of 6.6~7.8, and had 1h halflife at 45 ℃.
    β 葡萄糖苷酶的最适反应温度为60℃,最适pH为6.6,在pH6.6~7.8时比较稳定,保温1h酶活的半衰温度是45℃.
    The optimal conditions for cellobiohydrolase were pH 5.8 at 60 ℃, it was stable at pH 6.2, and had 1h halflife at 44 ℃.
    纤维二糖酶的最适反应温度为50℃、最适pH为5.8,在pH6.2最稳定,保温1h酶活的半衰温度是44℃.
    The optimal conditions for endoglucanase were pH 5.4 at 50 ℃, it was stable over a pH range of 6.6~7.0, and had 1h halflife at 58 ℃. 
    内切葡聚糖酶的最适反应温度为60℃,最适pH为5.4,在pH6.6~7.0时比较稳定,保温1h酶活的半衰温度是58℃.
    Two strain was selected for cultivation on the basis of the study of the growth rate mycelium and CMC enzyme activity of the hybrids.
    在对杂交菌株进行菌丝生长速率与CMC酶活研究基础上 ,分别选一代表株进行栽培试验。
    pH in medium varied between 4.25~5.37.The maximum biomass and total SOD activity in bioreactor were 16.3 gDW/L and 7.72×104U/L,73.69% and 71.35% respectively compared to that in flak culture.
    结果发现,大蒜细胞发酵罐中悬浮培养的pH变化趋势为先降后升,波动范围为4 25~5 37,获得最大生物量和SOD总酶活分别为16 3gDW/L和7 72×104U/L,相应为摇瓶培养的73 69%和71 35%。
    The optimal conditions for xylanase were pH 5.8 at 60 ℃, it was stable over a pH range of 5.4~7.0, and had 1 h half life at 55 ℃.
    木聚糖酶的最适反应温度为 6 0℃ ,最适反应 pH为 5 .8,在pH 5 .4~ 7.0时比较稳定 ,保温 1h酶活的半衰温度是 5 5℃ .
    The optimal conditions for xylosidase were pH 6.6 at 55 ℃, it was stable over a pH range of 6.6~7.4, and had 1 h half life at 52 ℃.
    木糖糖苷酶的最适反应温度为 5 5℃ ,最适反应 pH为 6 .6 ,在 pH 6 .6~ 7.4时比较稳定 ,保温 1h酶活的半衰温度是 5 2℃ .
    The optimal conditions for arabinofuransidase were pH 5.0 at 60 ℃, it was stable over a pH range of 4.6~6.2, and had 1 h half life at 60 ℃.
    阿拉伯糖苷酶的最适反应温度为 6 0℃ ,最适反应pH为 5 .0 ,在 pH 4 .6~ 6 .2时比较稳定 ,保温 1h酶活的半衰温度为 6 0℃ .
     Grifola frondosa was N~+ ion implanted by dose of 20×10~(13) ion/cm~2~100×10~(13) ion/cm~2, separated 5 treats and 0 treat as content. The effect was discussed through investigated mycelium growth characteristics, activity of cellulase, mycelium biomass, content of Grifolan etc.
    本文用20×1013ion/cm2~100×1013ion/cm2剂量的N+离子注入灰树花菌,设5个处理,0处理为对照,观测菌丝长势、菌丝长速、纤维素酶活、菌丝生长量、多糖含量探讨离子注入的效应与影响。
 

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