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    NR reactivity was many times of CK, root POD reactivity decreased by 22.4% than CK.
    硝酸还原酶活性是对照的几十倍,根系过氧化物酶活性比对照降低了22.4%;
    With Phenylalanine Ammonia-Lyase (PAL) and Stilbene Synthase (STS) as the main indicators of grape resistance to disease, the effects of main components of E26 agent on the activities and genes' transcriptions of PAL and STS of suspension cells of two grape varieties (qiouhei and chixiazhu) were studied. The objective was to determine whether there existed induced disease resistance in the mechanisms of E26 agent controlling the grape crown gall.
    以苯丙氨酸解氨酶(PAL)和1,2-二苯乙烯合酶(STS)作为葡萄抗病性的反应指标,对秋黑和赤霞珠两个葡萄品种的悬浮细胞在E26菌剂主要组分处理后的PAL酶活及防卫反应基因pal和sts的转录进行了研究,以探讨E26菌剂对葡萄抗病性的诱导作用。
    The results showed that, PAL activities were higher at most investigated hours after treated with the whole fermented liquid or metabolized liquid, while increased indistinctively and changed fluctuantly after treated with E26 cell or culture medium.
    试验结果表明,E26发酵液或代谢液处理后,PAL酶活在绝大多数时间显著高于对照; E26菌体或培养基处理后,PAL酶活升高不明显,且波动较大。
    SOD activity started to rise apparentlyat the first day and reached the maximum at the second day, with 52.42 unit enzyme activity higher than that of control.
    SOD活性在诱导处理后1d增加,第2d时酶活性达高峰,比对照高52.42酶活单位;
    The purified mutant enzyme showed a 3-fold increasing in o-nitrophenyl-galactopyanoside specific activity.
    以ONPG为底物的酶活测定结果显示,定向进化的β—半乳糖苷酶的活性相比于野生型,酶比活提高3倍。
    2. Using ultraviolet spectrophotometry determining the enzyme activity of POD,PPO,IAAO,PAL in jujube bearing branch, the result showed that in the same cultivar, there is no significant difference among all the single tree on the enzyme change tendency. But in Pozao cultivar, the POD,PPO,IAAO enzyme activity of susceptible single tree are a little higher than resistant ones, the PAL enzyme activity lower than resistant ones.
    2.用分光光度法对枣树枣吊内POD、PPO、IAAO、PAL酶活性进行测定,结果表明同一枣树品种不同单株间四种酶活性的变化趋势在生长季节中没有太大的差异,但是婆枣中感病单株的POD、PPO、IAAO酶活性比抗病单株高,PAL酶活性比抗病株低;
    While in Hupingzao cultivar, the POD,IAAO activity of susceptible tree are higher than resistant ones, the PPO,PAL activity lower than resistant ones.
    胡瓶枣中感病株POD、IAAO酶活性比抗病株高,PPO、PAL酶活性比抗病株低。
    dynamic variation of diastase, CMC enzyme, cellulase during the submerged culture were quite similar;
    淀粉酶、CMC酶、纤维素脱脂棉酶与滤纸酶的酶活变化趋势相近;
    Glucose and fructose were mainly accumulated in sugar accumulation of grape berries. Compared to Cabernet Sauvignon, Shang-24 sugar content was lower, but its activity of sugar accumulation related-enzyme was higher. This resulted from the high PAL activity, which made a number of sugar participation in biosynthesis of phenolic compounds in Shang-24 berries.
    葡萄果实糖的积累主要以葡萄糖和果糖为主,毛葡萄商-24与欧亚种葡萄赤霞珠相比,其果实糖含量相对较低,而与糖积累相关的酶活性高,其原因是由于商-24果实中高的PAL酶活使其大量的糖分参与了酚类物质的合成。
    After the treatment of the stress stimulus on leaves, the activities of resistance-related enzymes were significant increased.
    黄瓜叶片经局部应力刺激后,其体内抗病性相关的酶活明显升高。
    In vivo, the peak of PMG activity appeared formerly and the peak of Cx activity appeared later whether in resistant variety or in susceptible ones after inoculation.
    2.2 活体内细胞壁降解酶活性的变化 活体内,无论抗感品种接种炭疽菌后,均先出现 PMG 活性高峰,后出现 Cx 活性高峰; 抗病品种的细胞壁降解酶活性要低于中感和感病品种,但酶活高峰出现较早。
    and furthermore, the APX activity of the cotyledonary embryoid was at the peak, and it should be the total of the activities of the different soluble APX isoenzymes.
    龙眼体胚各个阶段的同步化材料都检测到了APX的活性,并且在子叶形胚时的酶活性最高,此酶活应该是可溶性的APX的多种同工酶活性的总和。
    When the capsicum fruit was inoculated with filtrated supernatant fluid, POD activity was 22.73% higher than that of water control.
    粗提液诱导8d后接种疫霉的处理,在粗提液处理到接种疫霉期间,β-1,3-葡聚糖酶和外切几丁质酶活性大幅度上升,在接种疫霉时,辣椒苗的β-1,3-葡聚糖酶酶活比其它处理的高81.72%~95.37%,外切几丁质酶活性也比其它处理的高36.24%~57.58%。
    Furthermore the paper studied effect of soaking peanut seed with biogas liquid.
    同时研究了沼液用于浸种,对花生种子发芽率、发芽势、芽性状及种子酶活的影响。
    1.Using the different methods to mensurate the activity of PAL, PPO and POD, we have acquired the proper method to mensurate the activity of PAL, PPO, POD.
    1.对辣椒叶片苯丙氨酸解氨酶、多酚氧化酶及过氧化物酶酶活测定方法进行了探索,得到了适合辣椒叶片三种酶活测定的方法。
    The activity of enzyme shows that the three phenolic acid all can restrain MnP and Lip what are most substances of oxygenase of P. chrysosporium .
    酶活测定表明,三种酚酸对黄孢原毛平革菌胞外氧化酶系的主要成员MnP(锰过氧化物酶)和Lip(木素过氧化物酶)都有一定的抑制作用。
    However,variations of enzyme activities of POD,PPO,PAL,CAT and SOD were closely related to disease resistance of strawberry after inoculation.
    抗病性强的品种杜克拉、童子一号和卡尔特一号的超氧化物歧化酶(SOD)酶活高峰接种后8d出现,过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、过氧化氢酶(CAT)酶活高峰在接种后12d到达,酶活性提高幅度大;
    In resistant variety,the maximum value of SOD content was appeared in 8d after inoculation, the maximum value
    感病品种红实美和丰香的酶活高峰则在接种后出现地要比抗病品种迟,分别在12d和16d才出现,而且升幅小于抗病品种。
    The results showed that the chitinase activities of banana fruit treated with 1-MCP were obvious rising from the 12rd,reached the peak at 25rd. , were later 4 days than CK and the peak value also lower than CK .
    结果表明1-MCP处理的酶活在第12天开始明显提高,第25天达到最高峰,与无处理果实相比其几丁质酶活性峰延迟了4天,酶活性也明显降低。
    By contrast ,The activities of banana fruit treated with ethylene were obvious rising from the 2rd. reached the peak at 6rd. were eralier 13 days than CK and the peak value also higher than CK.
    乙烯利处理的酶活在第2天开始明显提高,第6天达到最高峰,与无处理果实相比其几丁质酶活性峰提前了13天,酶活性也较高。
 

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