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    We also analyzed methylation status of p73 exon 1 by restriction-enzyme related PCR (REP), methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) analysis in 63 ALL cell lines.
    应用DNA甲基化酶谱测定(REP)、双亚硫酸盐修饰的PCR(MSP)和PCR产物测序(BGS)等技术对63例急性淋巴细胞白血病细胞系p73基因第1外显子的甲基化进行了检测。
    Objective To observe the dynamic changes of serum soluble Fas (sFas) and soluble ligand of Fas (sFasL) of newborn with hypoxic-ischemic encephalopathy (HIE), and the relativity between the dynamic change , the coronal Computerized Tomography (CT) and serum zymogram so as to investigate the significance of apoptosis in newborn hypoxic-ischemic injury.
    目的 观察新生儿缺氧缺血性脑病(HIE)血清可溶性Fas(sFas)和可溶性Fas配体(sFasL)水平的动态变化,及与头颅CT的改变程度和血清酶谱水平间的相关性,借此进一步探讨细胞凋亡机制在新生儿缺氧缺血性脑损伤中的意义。
    There were12pa-tients in59children with changes of enzymogram within first12hours of life,and8cases(8/12,66.7%)within first6hours.
    12例(12/59)病人于出生后12h内出现心肌酶谱变化,而其中8例的酶谱变化出现于生后6h内。
    Besides, the methylation status of p73 exon 1 was analysed by restriction enzyme related PCR, methylation specific PCR and bisulfite genomic sequencing in the 61 ALL cell lines.
    应用DNA甲基化酶谱测定、亚硫酸氢钠修饰的PCR和PCR产物测序等技术对 61个ALL细胞系p73基因第 1外显子的甲基化进行检测。
    Gelatin zymography and ELISA were used to detect the activity and levels of serum MMP-9. MMP-9 mRNA expression in the leucocytes was detected using reverse transcription-polymerase chain reaction (RT-PCR).
    分别应用明胶酶谱法、酶联免疫吸附法检测血清MMP 9的活性和蛋白浓度 ,半定量RT PCR的方法检测外周血白细胞MMP 9mRNA表达水平。
    Gelatin zymography and enzyme-linked immunosorbent assay(ELISA) were used to detect the enzyme activity and total protein levels of MMP-9 in supernatant. MMP-9 mRNA expression in ECV-304 cells were studied by RT-PCR.
    应用明胶酶谱法、ELISA测定细胞培养上清中MMP 9活性和蛋白水平 ,RT- PCR法测定MMP 9mRNA表达。
    A Preliminary Observation on Changes of Cardiac Enzymes in Sclerema Neonatorum
    新生儿硬肿症心酶谱变化的初步观察
    Observation and Clinical Assessment of Cardiac Enzyme Spectrum Determination in 41 Children with Pneumonia
    心酶谱测定对41例小儿肺炎的观察与探讨
    Correlationship analysis of children's serious disease marking and serum-enzyme spectrum
    小儿危重症评分与血清酶谱的相关性分析
    THE CHANGES IN PLASMA ENZYME
    142例窒息新生儿血清有关酶谱的研究
    Diagnostic significance of cerebrospinal fluid zymogram on child atypical bacterial meningitis
    脑脊液酶谱对小儿不典型细菌性脑膜炎诊断意义的临床研究
    Objective To discuss the correlativity of children's serious disease marking and sero- enzyme spectrum with digital- analogue technic of computer and provite basis for earlier diagnosis and trentment of children's MODS and MSOF.
    目的用计算机数字摸拟技术探讨小儿危重病症评分与血清酶谱动态关系及临床意义,为小儿多器官损害综合症(MODS)及多器官衰竭(MSOF)的早诊、早治提供依据。
    Objective:To study the changes in plasma enzyme spectrum in asphyxiated neonates and evaluate their clinical significance.
    目的:研究新生儿窒息后血清有关酶谱改变对临床的指导意义;
    Results:A family with autosomal dominant BCMD affecting 6 members of both sexes in 3 generations was discri bed. The onset of symptoms was in early childhood and progression was slow. The proximal limb muscles was affected.
    结果:该家系3代共6个患者,患者肌无力症状在儿童早期即出 现,进展缓慢,以近肢端肌受累明显,心肌及中枢神经系统未受累,肌酶谱增高,肌电图检查示肌原性损害,肌活检 组织学观察发现明显的线粒体异常。
    Electrophoresis in discontinuous buffer system was applied for the presenting patterns and fractions of the pancreatic amylase isoenzymes.
    应用不连续缓冲系统薄膜电泳法行酶谱分析。
    Electrophosresis in discontinuous buffer system was applied for the presenting patterns and fractions of the pancreatic amylase isoenzymes.
    应用不连续缓冲系统薄膜电泳法行酶谱分析。
    Conclusion The determination of enzymi spectrim in serum takes some importance for judgement of prognosis and guide clinical treatment in HIE patients.
    结论 :检测的血清酶谱对 HIE预后判断的探讨及指导临床治疗有一定的重要意义
    Electrophoresis in discontinuous buffer system was applied to fractionalize the pancreatic amylase isoenzymes.
    应用不连续缓冲系统薄膜电泳法行酶谱分析。
    At 3,7 and 14 days after exposure, gelatinase activity in bronchoalveolar lavage fluid (BALF) of another six rats in each group by gelatin zymography was examed.
    暴露 3、7、14d ,每组另取动物 6只 ,酶谱法分析支气管肺泡灌洗液中明胶酶的活性。
    Data was analysised by variance analysis and the results indicated that P<0.05.12 cases had alternation of enzymongram within first 12 hours of life,and 8 cases(8/12,66.7%)within first 6 hours.
    12例 (12 /16 5 )病人于出生后 12h内出现心肌酶谱变化 ,而其中 8例的酶谱变化出现于生后 6h内。
 

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