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recombinant bcg
    Construction of Recombinant BCG Secreting IL-2 and IFN-α 2a and Immunotherapeutic Effect on Mice with Bladder Cancer
    分泌IL-2和IFN-α2a的基因重组卡介苗构建和抗膀胱肿瘤实验研究
    The pYUB295 recombinant BCG was identified by amplified recombinant BCG genome DNA and showed Ag85a gene was transformed into BCG successfully. The SDS-PAGE result of Ag85a recombinant BCG culture filtrate showed: the extrinsic Ag85a gene could be expressed in BCG.
    pYUB295-ag85a重组卡介苗基因组DNA的PCR扩增以及培养上清液的PAGE电泳表明:ag85a-卡介苗重组疫苗构建正确,Ag85a蛋白在卡介苗中分泌表达。
    INFECTION OF DENDRITIC CELLS BY RECOMBINANT BCG
    重组卡介苗感染树突细胞的研究
    Studies and Application of Recombinant BCG Vaccines
    重组卡介苗的研究与应用
    Construction, Expression and Identification of a Recombinant BCG Vaccine Encoding Human Mycobacterium tuberculosis Heat Shock Protein 65
    人结核杆菌热休克蛋白65重组卡介苗疫苗的构建、表达及鉴定
    Screening and construction of recombinant BCG strains expressing the Ag85B-ESAT6 fusion protein
    分泌表达Ag85B与ESAT6融合蛋白的重组卡介苗菌株的筛选
    Construction of A Recombinant BCG Vaccine Expressing the Secretory Form Fusion Protein 85B-ESAT-6
    分泌性表达融合蛋白85B-ESAT-6重组卡介苗的构建和表达
    Construction and Screen of Recombinant BCG Strain Expressing and Secreting Human Interleukin 12 Protein
    分泌表达人IL-12基因重组卡介苗菌株的构建和筛选
    Objective: To investigate the effect of recombinant BCG secreting IL-2 (rBCG-IL-2) on peritoneal macrophages of mouse and inquire into the effect of rBCG-IL-2 on immunity of host.
    目的:研究能分泌人白细胞介素2的重组卡介苗(rBCC-IL-2)对小鼠腹腔巨噬细胞体外杀伤活性功能的改变,探讨rBCG-IL-2对宿主免疫功能的影响。
    Objective To screen and construct recombinant BCG strains which express the Ag85B-ESAT6 fusion protein.
    目的筛选获得分泌表达Ag85B与ESAT6融合蛋白的重组卡介苗(BCG)菌株。
    Objective To construct a recombinant BCG capable to express the secretory form fusion protein 85B-ESAT-6. Method PCR amplified regulatory sequence of hsp60 from the genome of H37Rv strain and the synthesized T4 transcription-termination sequence were cloned into pBCG2000 vector,yielding the E.
    目的构建分泌性表达融合蛋白85B-ESAT-6重组卡介苗。 方法PCR扩增结核分枝杆菌热休克蛋白60(hsp60)的调控序列和合成T4转录终止序列,分别定向克隆入pBCG2000载体的多克隆位点的上下游,得到E.
    Construction of recombinant BCG bearing Schistosoma japonicum 26Ku antigen gene and study on its immunogenicity on mice
    血吸虫重组卡介苗-Sj26GST疫苗的构建及免疫原性的研究
    Effects of recombinant BCG and PPS on levels of nitric oxide released from peritoneal macrophages in tumor-bearing mice
    重组卡介苗及猪苓多糖对荷瘤小鼠巨噬细胞NO释放量的影响
    Construction of a recombinant BCG expressing cell wall-associated 27 000 outer membrane protein of Coxiella burnetii
    表达胞壁结合型贝氏柯克斯体27000外膜蛋白的重组卡介苗构建
    Under heat induced (45℃) , the expression recombinant protein was analyzed by SDS-PAGE and its relative molecular mass is 22kD. Our results may lay a foundation for constructing recombinant BCG vaccine against F. hepatica.
    45℃下热诱导FH3基因在重组卡介苗中的表达,并对基因表达产物进行SDS一PAGE分析,结果显示,重组卡介苗总蛋白在约22kD处有新的蛋白带出现,符合FH3基因序列表达产物大小。
    Methods Gene engineering and electric transformation technologies were adopted to construction recombinant BCG,one of which was rBCG-IL-2 strain and the other was rBCG strain. Observe the growth of tumor and determine tumoricidal effect of macrophage after the tumor cells and recombined BCG have been inoculated in mouse in a few weeks.
    方法 采用基因工程技术和电转化技术 ,构建重组卡介苗即rBCG IL 2和rBCG ,并用瘤株重组BCG接种荷瘤小鼠 ,几周后观察肿瘤生长情况和检测巨噬细胞的杀伤活性。
    Objective To construct a recombinant BCG secretively expressing ESAT 6 of Mycobacterium tuberculosis.
    目的 构建分泌性表达结核分枝杆菌抗原蛋白 ESAT- 6的重组卡介苗
    The infection and antigen presenting cell functions of dendritic cells (DC) with recombinant BCG expressing MalE protein (rBCG·MalE ) from Escherichia coli were investigated.
    在体内外试验中 ,树突细胞 (DC)均能摄取表达大肠杆菌麦芽糖结合蛋白 (MalE)的重组卡介苗 (rBCG·MalE)。
    Recombinant BCG (rBCG) has been used as a new vaccine vehicle because it can express antigens of multiple pathogens.
    重组卡介苗可同时表达多种病原体抗原 ,已成为新的疫苗载体。
    Objective To construct a recombinant BCG vaccine carrying heat shock protein 65 (HSP65) from human Mycobacterium tuberculosis (M.TB).
    目的 构建人结核杆菌热休克蛋白 6 5 (HSP6 5 )重组卡介苗 (BCG)疫苗。
 

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