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活力回收
    The optimum conditions of immobilized penicillin acylase,such as temperature, pH and amount of native enzyme, were studied. Under optimumexperimental conditions, the activity and activity recovery of immobilized enzymewere determined.
    我们研究了固定青霉素酰化酶的制备、固定化条件如温度、pH、酶量等对酶活力回收的影响,并对固定化酶反应动力学进行了探讨,考察了酶反应速度和酶与底物之间的动力学关系。
    The activity recovery of the immobilized laccase was 37% while its activity was 23 OD/min-g (dry).
    固定化漆树酶的活力达23光密度变化值/分·克(干),活力回收达37%。
    A porous silica treated by ZrCl_4 solution used as carrier of Rhus laccase immobilization was produced, the activity recovery and stability of the immobilized Rhus laccase is superior to that reported in literature, the retained activity was about 75% after it had been used for 20 times.
    研究表明,经ZrCl_4处理后的多孔硅胶作载体的固定化漆树酶活力回收高、稳定性好、重复使用20次其保留活力仍有75%。
    The activity recovery of the immobilized en- zyme approximatly was 69%.
    固定化酶的活力回收达69%。
    Results: PVA served as immobilized carrier of NO. WW-11, and its activity recovery of tryptophanase was 60. 9%.
    结果:以聚乙烯醇作为WW-11的固定化载体,其活力回收为60.9%。
    The β-glucanase from strain GXC of Trichoderma reesei was purified in three steps which comprised ammonium sulfate precipitation, Sephadex G-100 chromatography, and DEAE-Sephadex A-50 chromatography. The purified enzyme showed an activity increase of 14.60 fold, and an activity recovery of 6.62%.
    对里氏木霉所产β-葡聚糖酶粗酶液通过饱和硫酸铵沉淀、Sephadex G-100 柱层析和DEAE-Sephadex A-50 柱层析进行纯化,比活提高14.60倍,活力回收6.62%。
    The activity of immobilized enzyme was 891 U/g, and enzymatic activity recovery reached 359%. 
    制得的固定化酶活力为89 1U/g载体,酶的活力回收达到35 9%.
    RESULTS After optimization the activity of immobilized trypsin reached 6848.40 U·g -1 ,and its activity recovery reached 21.8%.
    结果 优化后制得的固定化胰蛋白酶的活力单位 (AU)高达 6 84 8.4U·g-1 ,活力回收 (AR)为 2 1 .8%。
    The result was 347.09U ACE was obtained from 200g hog lung tissue with specific activity 29.029U/mg and activity recovery12.53%.
    200g猪肺可制备出ACE347.09U,比活力29.029U/mg,活力回收12.53%。
    the temperature was 4 ℃ ; the immobilizing time was 6 h; and the pH value was 7.5. The activity and activity recovery of the immobilized papain were 1 786.93 U/g and 69.54% , respectively.
    研究结果表明,最佳的固定化条件为:给酶量0.36mg/g(蛋白质质量浓度为0.12 mg/mL)、pH 7.5、温度4℃左右条件下,固定6 h,所得到的固定化酶活力为1 786.93 U/g,活力回收高达69.54%.
    β-Galactosidase was immobilized on chitosan microspheres with glutaraldehyde by cross-linking reaction,the immobilezation conditions and characterization of the immobilized enzyme were studide. The optimal conditions for immobilization were as follows:in pH 6.5 P-E-M solution for 10 h,chitosan microsphere was treated with 0.5% glutaraldehde at 25℃ for 12h,the solution of β-Galactosidase was immobilized on the carrier at 4℃ for 12h, enzyme activity recovery was 67%.
    以壳聚糖微球为载体,戊二醛为交联剂,固定β-半乳糖苷酶,对β-半乳糖苷酶的固定化条件及固定化酶的各种性质进行了研究,确定了酶固定的最适条件为:用pH6.5的P-E-M缓冲液浸泡10h,25℃壳聚糖微球与0.5%戊二醛交联12h以上,4℃下酶与壳聚塘微球固定12h以上酶活力回收可达67%。
    The results of the experiment demonstrated that the optimum conditions for immobilization were as follows: the range of optimum concentration of solution papain was 1.0-(2.0 mg/mL),pH value was 7.0 and the time for immobilization was 2 h. The maximal activity of the immobility papain was 111.1 U/g and the activity recovery of the immobility papain was 57.9%.
    试验结果表明,当溶液酶质量浓度为1.0~2.0 mg/mL、pH 7.0、固定2 h时,固定化酶活力最高达111.1 U/g,活力回收达57.9%.
    The experiment carried the research on the effects of different coupling time, pH of buffer, the amount of cellulase and ionic strength on immobilized cellulase with four targets: activity, specific activity, protein binding capacity and activity recovery.
    实验以酶活力、比活力、蛋白载量和活力回收为指标,探讨了给酶量、反应pH值、反应时间、离子强度等因素对固定化纤维素酶的影响。
    The influence of immobilized conditions (such as irradiationdose, polymerization temperature, amount of yeast and cross-linking reagent) on theenzymatic activity has been investigated. From the experiments it was found that theirradiation dose until 2× 10~6 rad has not a significant effect on the enzymatic activi-ty. The immobilized cell which was prepared according to the optimum conditionswas not only elastic but also hard and exhibited higher activity recovery.
    丙烯酰胺作为载体,NN‘-甲撑双丙烯酰胺作为交联剂。探讨了固定化的一些条件,如辐照剂量、聚合温度、酵母和交联剂的用量对酶活力的影响。试验发现,辐照剂量高达2×10~6rad,对酶活力没有影响。由最适条件所制得的固定化细胞既有弹性又有硬度,并且显示较高的活力回收,重复10次反应后酶活力保持不变。
    It is found that activity recovery of the immobilized laccase was about 30%.
    固定化漆树酶的活力回收可达30%;
    The crude extract was purified by 218 folds. The activity recovery, calculated from urine, was 12 percent.
    大分子尿激酶占90%以上,纯化系数为218,活力回收按原尿计为12%。
    The method of PEG/phosphate salt two phase extraction as the first step of purifi-cation to prepare duck serum choline esterase was first used in this paper. The procedurewas not only simple,rapid but also high in the activity recovery of the choline esterase. The purified choline esterase with specific activity 279.9 U/mg was followed by DEAE-sephadex A50 and sephadex G200 chromatography.
    首次采用新技术双水相萃取方法作为鸭血清胆碱酯酶(EC.3.1.1.8 CHE) 纯化的第一步,后经 DEAE-Sephadex A50,sephadex G200 柱层析,获得电泳纯鸭血清胆碱酯酶,提纯倍数1018倍,酶活力回收43.4%,比活274.9U/mg。
    It is a effective carrier for the simple andrapid immobilization of various enzymes andproteins. The amounts of bound protein(BSA) and glycoamylase in one gramme of thedry carrier were 558mg and 330mg respective-ly,the activity recovery of 84/00 for immobi-lized glycoamylase was obtained.
    可作为载体对多种酶及蛋白质给予固定,与蛋白质的最大结合量为558mg/g,固定糖化酶时的活力回收达84%;
    The duck serum CHE was immobilized by agar with the different hydrophobic groups. The optimum conditions for immobilization were studied,and 7-76% activity recovery of the immobilized CHE was obtained.
    首次摸索了带有疏水基团琼脂珠固定化鸭血清胆碱酯酶的最佳条件,固定化酶活力回收为70一76%。
    Lysozyme was separated from pupae by following steps, heat denaturation, isoelectric precipitation, DEAE-cellulose ion-exchanged column chromatography and chitin-coated cellulose affinify chromatography the relative activity of the lysozyme preparation was 36700 units/mg protein, which was 150 times of the control. The total activity recovery rate was 48.6%.
    应用选择性热变性、等电点沉淀、DEAE—纤维素离子交换层析、CC—纤维素亲和层析等分离技术从柞蚕蛹血淋巴中提取溶菌酶,溶菌酶比活力达36700μ/mg蛋白,提高了150倍,活力回收达48.6%。
 

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