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活性回收率
    The results showed the optimum immobilized α-Acetolactate decarboxylase being 20 mL/g the magnetic microspheres,0.98 % glutaraldehyde,in which the activity of immobilized α-Acetolactate decarboxylase was 65 180 U/g,the specific activity was 872.32 U/mg and the activity recovery was 42.33%.
    结果显示,在酶固定化过程中,自由酶的添加量为20 mL/g微球,戊二醛的添加量为0.98%。 在其固定化最佳条件下,制备的固定化ALDC的活力为65 180 U/g,而且其比活、活性回收率分别可达872.32 U/mg和42.33%。
    The protein mass yield of rhIFNα-2b under the optimum condition was 4.2 times higher than that of dialysis refolding process. The activity recovery rate was 68.7% with the specific activity of 1.77×108IU/mg,and purity of 90%.
    结果表明,复性后rhIFNα-2b的蛋白收率是透析复性法的4.2倍,活性回收率为68.7%,比活力1.77×108IU/mg,纯度高于90%。
    It was shown that the specific activity of immobilized trypsin on chitosan was 3 936.4 6BAEE/mg,and the activity recovery of trypsin was 70.81%. The activity rate of aprotinin is 114.37 times higher than that of the tissue extrac.
    抑肽酶抑制比活单位(BAEE/mg)为3 936.46,酶活性回收率70.81%,最后的纯化倍数达到114.37倍。
    The result showed that the activity of immobilized trypsin on the crosslinked chitosan microsphere was 1270 KIU·g-1, 52.4 %protein was coupled, and the activity recovery of trypsin was 50.8 %.
    结果表明,制备的亲和吸附剂单位活力1270KIU·g-1,蛋白质偶联率52.4%,酶活性回收率50.8%。
    The activity recovery of the protein that obtained on this column exceeds 90%.
    蛋白质在此柱上可得90%以上的活性回收率
    The specific activity,recovery and molecular weight were 23.7IU/mg,13.67% and 147.748 9 ×10-24kg respectively. r-SK and plasminogen were mixed,modified by p-Amidinophenyl-p'-Anisate. HCl(APAN) and applied to a L-lysine-sepharose 4B collumn.
    人新鲜血浆经Lysine-Sepharose4B亲和层析,SephadexG-25凝胶过滤制备纤溶酶原(plg),比活性达23.7IU/mg,活性回收率为13.6%,SDS-PAGE显示分子量为147.7489×10-24kg的一条带。
    The result showed that under adquate conditions of temperature (35℃), pH (pH 1. 5), ionic strength (1.0 mol/L KCl) and absorbtion state (176 FIP/g), the activity recovery of purified aprotininum was about 83% and the specific activity was more than 5 000kIU/mg.
    该方法在适当的温度(35℃)、pH值(pH1.5)、离子强度(1.0mol/LKCI)以及吸附条件(176FIP/g)下,纯化的抑肽酶活性回收率达83%,比活可达5000kIU/mg以上。
    coli was purified by HPAC. The purity of purified rhIL 2 was 95% by SDS PAGE with Coomassie Blue. The activity recovery and specific activity were 92% and 2.0×10 9 IU·g -1 respectively.
    结果 使用该填料对大肠杆菌表达的 rh IL - 2进行了纯化 ,纯化后的 IL - 2经 CTL L细胞活性测定和 SDS- PAGE的纯度测定 ,确定其活性回收率为92 % ,比活为 2 .0× 10 9IU· g- 1 蛋白质 ,纯度为 95 % .
    RESULTS 2 49 mg final product was homogeneous by the criterion of SDS PAGE representing the 12.46% yield and 13.68% activity recovery from 1000 mL serum.
    结果 成功地从 1L血浆中提纯到了 2 .49m g C2 ,产率为 12 .46 % ,活性回收率为 13.6 8% .
    The purity ,specific activity,recovery rate of activity,relative molecular weight (M.W)and PI were over 98%,2.4×10 7IU/mg, 14%,19800 and 5.0 respectively .
    纯化的rhIFN α1b的纯度为 98%以上 ,比活性 2 .4× 10 7IU/mg ,活性回收率 14 % ,相对分子质量 1980 0和等电点 5 .0。
    The result showed:the specific activity of immobilized trypsin on chitosan was 79 000 BAEE/mg,and the activity recovery of trypsin was 76.63%.
    通过实验研究,计算抑肽酶抑制比活单位(BAEE单位/mg胰蛋白酶)为79000,酶活性回收率76.63%。
    The total activity recovery of this purification method was 52.6% and the purification factor was 3.87.The final specific activity of recombinant NbAChE was 2837(U/mg).
    该方法的活性回收率为52.6%,纯化因子为3.87; 纯化后AC hE的比活为2 837U/m g。
    After influencing factors such as pretreatment of lysate,dosage of sample,dilution folds and elution condition were discussed,immunomagnetic separation methods was established in this paper. The purity of IFN α-2b by one step was over 99%,activity recovery was 88% and specific activity was 1.5×108 IU/mg.
    通过对α-2b干扰素发酵菌体裂解液的预处理、上样量和稀释倍数以及洗脱条件进行考察,确立免疫磁性分离条件,用于α-2b干扰素的一步分离纯化,其活性回收率达到88%,平均比活性为1.5×108IU/mg,纯度大于99%。
    The result shows: the specific activity of immobilized trypsin on chitosan is 71 428 BAEE/mg,and the activity recovery of trypsin is 62.5%.
    抑酶肽抑制比活力为71 428 BAEE. mg-1,酶活性回收率为62.5%。
    The SOD of electrophoretic rank was prepared by the method with total activity recovery of 86.0%,52 times purification.
    利用此法制得电泳级的SOD,总活性回收率为86.0%,纯化倍数提高52倍。
    Results Through dye affinity chromatography column immobilized with Cibacron Blue F3GA,EDH purification factor was 2.0,activity recovery was 30.8%;
    结果经过固定有Cibacron Blue F3GA的亲和层析柱,乙醇脱氢酶的纯化倍数为2.0,酶活性回收率为30.8%;
    and through dye affinity chromatography column immobilized with Red KE-3B,G-6-PDH purification factor was 23.2,activity recovery was 66.0%.
    经过固定有KE-3B艳红的亲和层析柱,葡萄糖-6-磷酸脱氢酶的纯化倍数为23.2,酶活性回收率为66.0%。
    But the mass recovery and activity recovery of proteins refolded on the HPIEC are lower than that of on the HPHIC.
    但是只有3种蛋白获得复性,而且质量和活性回收率普遍低于HPHIC,Z值、保留时间虽然与天然蛋白相近,但峰型相对较差,复性条件的选择也较HPHIC困难。 蛋白质在疏水作用色谱上的复性效果优于离子交换色谱。
    Human recombinant 1FN-α expressed in E. coli was purified with the column packed with the prepared adsorbent. The activity recovery was 94% and the purification factor was about 79. The final specific activity of lFN-a was 7. 94 × 106 IU/mg.
    用合成的α干扰素单克隆抗体高效液相亲和介质纯化由大肠杆菌表达的基因重组人α干扰素,层析过程的活性回收率为94%,纯化倍数为79倍,比活为7.94×106IU/mg。
    Results: Both the recovery rate(%) and immunological titers of anti hCG IgG purified with spacer arm sepharose 4B were increased as compared with those obtained when sepharose 4B without spacer arms was used (the activity recovery rate and specific activity increased by 22.5% and 55.6% in average respectively).
    结果:用该种亲和层析介质纯化的抗hCG抗体IgG回收率及免疫滴度均高于无手臂的亲和层析介质(活性回收率平均增加22.5%,比活性平均增加55.6%)。
 

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