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亲和性
    The fluorescence intensity of MSC-FITC-P1 was 29.7 times higher than that of controls, and about 99.8% of MSCs was conjuncted with FITC-P1. On the results of ELISA, the value of Bio-Pl bound to MSCs was 4.05 times higher than that of blank, which confirmed the favorable affinity of Bio-Pl towards MSCs.
    流式细胞仪检测FITC标记多肽与MSCs的结合率为99.8%,荧光强度为阴性对照的29.7倍。 酶联免疫吸附实验检测结果显示,生物素标记亲和肽(Bio-P1)与MSCs的结合强度为空白对照的4.05倍,说明Bio-P1与MSCs具有良好的亲和性
    In order to investigate the affinity and stability of Bio-Pl towards MSCs in vivo, MSCs-Bio-P1 was implanted into the periphery of tumor tissue below the skin of nude mouse.
    将MSC-Bio-P1植入裸鼠肿瘤组织旁侧,4天后剪切肿瘤组织进行冰冻切片免疫组化分析,结果显示MSCs阳性染色,说明生物素标记亲和肽与MSCs在体内有良好的亲和性与稳定性。
    HAb18 is a high affinity monocloonal antibody against hepatocellular carcinima membrane protein.
    HAb18是一种特异性较强的高亲和性肝细胞肝癌膜蛋白单克隆抗体,以往的实验证明,该抗体在荷瘤裸鼠及肝癌患者体内都有较好的定位。
    in the hybrids between 681A and three types,the affinity index of 681A was higher than it's maintain lines,which showed the affinity of hybridization has sterile cytoplasm effection.
    1)种间杂交的亲和性:a、利用油菜雄性不育系681A和其保持系与三大类芸薹属品种(红菜薹,白菜型油菜, 白菜)杂交,681A与三类品种杂交的亲和指数都高于其保持系。
    Recombinant Protein(rProtein) A Sepharose Fast Flow is an affinity medium. The capacity of specific binding for Fc region of IgG is enhanced by coupling protein A with Sepharose 4 Fast Flow. The IgG is perfectly purified by the covalent combination.
    Recombinant Protein(rProtein)A Sepharose Fast Flow是一种亲和性介质,重组蛋白A与Sepharose Fast Flow偶联后,增强了特异性结合IgG Fc段的能力,这种共价结合使抗血清中的免疫球蛋白IgG得到高度纯化。
    The results indicated that N-Succinyl-Chitosan demonstrated high affinity for K562 leukemia cell declining with the increasing of MW of chitosan.
    实验结果表明N-琥珀酰壳聚糖和K562肿瘤细胞间存在着较强的亲和性,可以作为抗肿瘤药物的靶向载体,并且随着分子量的增加,其亲和性逐渐减弱。
    To solve the problem in purification of recombinant fusion protein, affinity tag(6×His tag) is constructed in recombinant fusion protein to purify it by nickel chelating affinity chromatography.
    为了解决重组融合蛋白的纯化问题,人们将亲和性标签(6×His tag)构建到重组融合蛋白上,以便用镍亲和层析的方法纯化重组蛋白。 镍亲和层析是一种常用的蛋白质纯化方法,它利用金属镍(Ni)与6×His tag之间的亲和性,采用亲和层析的方法就可以将带有6×His tag的蛋白质纯化出来。
    In addition to the C3 pathway, C4 plants use the C4 photosynthetic cycle to elevate the CO2 concentration at the site of Rubisco. One of the key enzymes in C4 pathway is phosphoenolpyruvate carboxylase (PEPC) that has higher affinity for CO2 than Rubisco.
    C_4植物除了C_3途径外,还利用C_4光合循环在Rubisco位点提高CO_2浓度,其中一个关键酶是磷酸烯醇式丙酮酸羧化酶(PEPC),它与CO_2的亲和性比Rubisco高。
    The Km of modified L-asparaginase (Km(app) = 0.844><10"3 mol-L"1) was 5.6 times lower than that of native enzyme. The affinity of modified enzyme to substrate L-asparagine elevated.
    自然酶表观米氏常数K_m=4.765×10~(-3)mol·L~(-1),为修饰酶(K_(m(app))=0.844×10~(-3)mol·L~(-1))的5.6倍,表明修饰后酶对底物的亲和性提高。
    TGF-βRⅢ transfers TGF-βs in stroma to TGF-βRⅡ and enhances their affinity.
    TGF-βRⅢ主要负责将间质中的TGF-βs传递给TGF-βRⅡ并增强它们之间的亲和性
    2.The study of the relationship between expression of DsNRT2.1 and NO3-influx has shown that DsNRT2.1 has a higher affinity at low NO3- concentration.
    2.证实克隆获得的盐生杜氏藻DsNRT2.1基因编码一条高亲和性的硝酸盐转运蛋白。
    The newer formed AlN behaves finer interface affinity with Al-Si alloy.
    新生成的AlN与Al-Si合金相之间表现出很好的界面亲和性
    Zur (Zinc uptake regulator) exists in many bacteria. The primary function of Zur is repressing the high affinity zinc uptake system when the zinc concentration in the cell reaches a threshold. It has been reported that Zur is encoded by ORF XC1430 in Xanthomonas campestris pv.
    锌吸收调控蛋白(Zinc uptake regulator, Zur)存在于许多细菌中,它的主要作用是当细胞内的锌离子浓度过高时抑制高亲和性锌吸收系统(high affinity zinc uptake systems)的表达;
    Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃. HE97 was primarily regard as self-incompatible line in this research.
    通过分期播种,进行了光温敏自交不亲和材料HE97亲和性转换区间的研究,依据田间跟踪调查叶龄及室内考种研究了HE97亲和性转换区间的光温临界值,结果表明:1)日最低温度是影响HE97自交不亲和性的主要原因,亲和性转换区间在16.5℃~18.1℃之间。
    In this research, the NH_3/O_2 plasma and plasma grafting after Ar plasma treatment were used to modify its surface in order to improve its hydrophilicity, and discussed the affection on the amino density of material surface, the grafting density of PVP, the surface form before and after treatment, the change of contact-angle by different treatment conditions and make research on the cell affinity of modified materials.
    本文采用NH_3、O_2等离子法直接作用和Ar等离子处理接枝聚乙烯吡咯烷酮(PVP)的方法对其进行处理,以改善其表面亲水性,探讨了不同处理条件对材料表面氨基密度,PVP的接枝密度,处理前后材料的表面形态,表面接触角的变化的影响,并对改性后的材料的细胞亲和性进行了研究。
    and the 3T3 fibroblast cells culture appeared that the cells were growing best on the surface treated by the NH_3/O_2 plasma, and the cell affinity was improved in a certain extent on the surface treated by the plasma grafting.
    而对3T3成纤维细胞在材料表面的培养情况表明,利用NH_3、O_2等离子法处理的材料表面的细胞生长情况最好,用等离子接枝改性的材料表面的细胞亲和性也得到一定的改善。
    Consistently, we showed that Dimethylsulfoxide dramatically elevated the RNA polymerase activity and slightly increased the affinity (K_M) with the DNA template.
    与之相一致的是,我们的数据显示了二甲亚砜极为显著的提高了RNA聚合酶的活性,同时,并不改变RNA聚合酶对DNA模板的亲和性(K_M值)。
    Incidence of RSV rised to about 90.0% while initial incidence was 16.4% in high affinity transmitter group.
    经生物学接种鉴定高亲和性灰飞虱群体的发病率从F0代的16.4%上升到F2代的93.0%,F3到F7代都维持在90.0%左右。
    Incidence of the low affinity transmitter group fell to about 2.5%.
    低亲和性灰飞虱群体的发病率则一直维持在1%—2.5%。
    High affinity transmitter group is significant for the research of virus transmission mechanism, variability of pathogenicity of RSV、 varieties resistance.
    说明构建的高亲和性群体不仅具有很高的带毒率而且具有较好的遗传稳定性,这样一个群体对于传毒机制的研究、RSV致病性分化研究和品种抗性分析都具有重要意义。
 

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