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    RNA Interference to Exogenous Green Fluorescent Protein Gene Expression in Africa Green Cells Using siRNA
    非洲绿猴肾细胞外源绿色荧光蛋白基因表达的RNA干扰
    Objective:To investigate whether shiga toxin Ⅱ e varriant and ricin induce apoptosis in Africa green monkey kideny cell line Vero, and the expression of gene Caspase-9, Bax, Caspase-3.Methods : The raw Stx2e were isolated in the 40 to 80 fraction of ammoniumsulfate precipitation .
    目的:探讨志贺毒素Ⅱ型e突变体(shiga toxin Ⅱe varriant,Stx2e)和蓖麻毒素(ricin)诱导非洲绿猴肾细胞系Vero细胞凋亡的作用及其相关凋亡基因的表达。
    This paper described the cell killing activity of toxin A of Clostridium difficile on four cell lines which were Vero(Africa green monkey kidney),TPC-1 (human thyroid papillary carcinoma),NIH 3T3 (mouse fibroblast)and NIH 3T3 ras (NIH 3T3 cell transfected with ras oncogene)cell line.
    本文报道艰难梭菌A毒素对4种培养细胞的细胞致死活性的探讨。 4种培养细胞为Vero(非洲绿猴肾细胞)细胞、TPC─1(人甲状腺肿瘤细胞)细胞、NIH3T3细胞(小鼠成纤维细胞)及将ras癌基因转基因于NIH3T3细胞的NIH3T3ras细胞。
    3. Expression of CD81 gene in different eukaryotic cell linesThe recombinant vector pVAXl-CD81 and pcDNA3.1-CD81 were transfected into Africa green monkey kidney COS-7 cell line and human hepatocellular carcinoma HepG2 cell line respectively. And the transient expression products in the transfected cells were detected byimmunofluorescence assay (IFA), immunocytochemical staining andFACS.
    通过脂质体介导,将pVAXI一cD81转染至非洲绿猴肾细胞系COS一7细胞,pcDNA3.1一CD81转染至人肝癌细胞系H即仇细胞,采用间接免疫荧光法(IFA)检测CD81基因在COS一7细胞中的表达,采用免疫组织化学方法(胡C法)及流式细胞技术(FACS)定性及定量的检测CD81基因在HepGZ细胞表达状况。
    The polymer having phenylalanine or tryptamine on its surface had low cell toxicity in low dosage and high transfection efficiency in Africa green monkey kidney cell line when R+/-=5.At last, PAMAM, which have Phe and Trp as its graft, could effect plasmid condensation and enhance transfection efficiency.
    在低的浓度下,聚合物有相对较弱的毒性,在电荷比例为5的时候,修饰Phe和Trp的聚合物能够大大提高绿色荧光蛋白在非洲绿猿肾细胞中的表达效率。
    Africa green monkey cell (Vero) is the adapt cell of some kind of virus.
    非洲绿猴肾细胞 (Vero)是多种病毒的适应细胞。
 

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